Phospho-SMURF1 (Ser200) Antibody - #AF4323
| 製品: | Phospho-SMURF1 (Ser200) Antibody |
| カタログ: | AF4323 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-SMURF1 (Ser200) |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 55-90kD; 86kD(Calculated). |
| ユニプロット: | Q9HCE7 |
| RRID: | AB_2844402 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4323, RRID:AB_2844402.
折りたたみ／展開
E3 ubiquitin-protein ligase SMURF1; hSMURF1; KIAA1625; Smad specific E3 ubiquitin ligase 1; SMAD specific E3 ubiquitin protein ligase 1; Smad ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1; SMUF1_HUMAN; SMURF 1; smurf1;
免疫原
- Q9HCE7 SMUF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDGSGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLSNAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGTGGSVVDCRGLLENEGTVYEDSGPGRPLSCFMEEPAPYTDSTGAAAGGGNCRFVESPSQDQRLQAQRLRNPDVRGSLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPSPSGTIPGGDAAFLYEFLLQGHTSEPRDLNSVNCDELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLPLPSEGSLEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYMLQINPDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPELHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLKHCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9HCE7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y66 | Phosphorylation | Uniprot | |
| Y69 | Phosphorylation | Uniprot | |
| T145 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| Y251 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| T332 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| Y413 | Phosphorylation | Uniprot | |
| K418 | Ubiquitination | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| S656 | Phosphorylation | Uniprot | |
| K739 | Ubiquitination | Uniprot |
研究背景
E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS. Plays a role in dendrite formation by melanocytes.
Auto-ubiquitinated in presence of NDFIP1. Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
Cytoplasm. Cell membrane>Peripheral membrane protein>Cytoplasmic side.
Expressed in melanocytes.
Interacts with TRAF4. Interacts (via HECT domain) with FBXL15 (via LRR repeats). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation. Interacts with MAVS; the interaction is mediated by NDFIP1.
The C2 domain mediates membrane localization and substrate selection.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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