Phospho-NAKED2 (Tyr247) Antibody - #AF4347
| 製品: | Phospho-NAKED2 (Tyr247) Antibody |
| カタログ: | AF4347 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-NAKED2 (Tyr247) |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Horse, Dog |
| 分子量: | 50kDa; 50kD(Calculated). |
| ユニプロット: | Q969F2 |
| RRID: | AB_2844426 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4347, RRID:AB_2844426.
折りたたみ／展開
hNkd2; Naked 2; Naked-2; nkd2; NKD2_HUMAN; Protein naked cuticle homolog 2;
免疫原
- Q969F2 NKD2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRARDKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGPGGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVNHSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAHVRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSPPVQAKQEPQGRASHLQARSRSQEPDTHAVHHRRSQVLVEHVVPASEPAARALDTQPRPKGPEKQFLKSPKGSGKPPGVPASSKSGKAFSYYLPAVLPPQAPQDGHHLPQPPPPPYGHKRYRQKGREGHSPLKAPHAQPATVEHEVVRDLPPTPAGEGYAVPVIQRHEHHHHHEHHHHHHHHHFHPS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q969F2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| G2 | Myristoylation | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| Y256 | Phosphorylation | Uniprot | |
| T257 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| Y423 | Phosphorylation | Uniprot |
研究背景
Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity (By similarity). Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells.
Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25.
Cell membrane. Cytoplasm. Cytoplasmic vesicle.
Expressed in kidney, lung, pancreas and spleen.
Interacts with DVL1, DVL2, DVL3 and PPP2R3A (By similarity). Interacts with RNF25 and TGFA (via cytoplasmic domain).
The N-terminal domain comprising the first 217 amino acid residues is mostly unstructured.
Belongs to the NKD family.
研究領域
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
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