Phospho-ENSA (Ser67) Antibody - #AF4381
| 製品: | Phospho-ENSA (Ser67) Antibody |
| カタログ: | AF4381 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-ENSA (Ser67) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 15kDa; 13kD(Calculated). |
| ユニプロット: | O43768 |
| RRID: | AB_2844446 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4381, RRID:AB_2844446.
折りたたみ／展開
Alpha endosulfine; ARPP 19e; Endosulfine alpha;
免疫原
Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas.
- O43768 ENSA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQKQEEENPAEETGEEKQDTQEKEGILPERAEEAKLKAKYPSLGQKPGGSDFLMKRLQKGQKYFDSGDYNMAKAKMKNKQLPSAGPDKNLVTGDHIPTPQDLPQRKSSLVTSKLAGGQVE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43768 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| Y41 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K47 | Acetylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| K56 | Methylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| K89 | Acetylation | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| T93 | O-Glycosylation | Uniprot | |
| T99 | O-Glycosylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| K114 | Ubiquitination | Uniprot |
研究背景
Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA.
Cytoplasm.
Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas.
Interacts (when phosphorylated at Ser-67) with PPP2R2D (By similarity). Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109.
Belongs to the endosulfine family.
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