Phospho-FRA1 (Ser265) Antibody - #AF4410

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製品: Phospho-FRA1 (Ser265) Antibody
カタログ: AF4410
タンパク質の説明: Rabbit polyclonal antibody to Phospho-FRA1 (Ser265)
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 43kDa; 29kD(Calculated).
ユニプロット: P15407
RRID: AB_2844475

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-FRA1 (Ser265) Antibody detects endogenous levels of FRA1 only when phosphorylated at Ser265.
RRID:
AB_2844475
引用形式: Affinity Biosciences Cat# AF4410, RRID:AB_2844475.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FOS L1; FOS like antigen 1; Fos related antigen 1; Fos-related antigen 1; FOSL 1; FOSL1; FOSL1 protein; FOSL1_HUMAN; FRA 1; FRA-1;

免疫原

免疫原:
Uniprot:

P15407(FOSL1_HUMAN) >>Visit HPA database.

遺伝子(ID):
FOSL1(8061)
タンパク質配列:
MFRDFGEPGPSSGNGGGYGGPAQPPAAAQAAQQKFHLVPSINTMSGSQELQWMVQPHFLGPSSYPRPLTYPQYSPPQPRPGVIRALGPPPGVRRRPCEQISPEEEERRRVRRERNKLAAAKCRNRRKELTDFLQAETDKLEDEKSGLQREIEELQKQKERLELVLEAHRPICKIPEGAKEGDTGSTSGTSSPPAPCRPVPCISLSPGPVLEPEALHTPTLMTTPSLTPFTPSLVFTYPSTPEPCASAHRKSSSSSGDPSSDPLGSPTLLAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15407 基板として

Site PTM Type Enzyme
R66 Methylation
T69 Phosphorylation
S74 Phosphorylation
R79 Methylation
R84 Methylation
S101 Phosphorylation
K144 Ubiquitination
T183 Phosphorylation
S191 Phosphorylation
T217 Phosphorylation
T223 Phosphorylation Q04759 (PRKCQ)
T227 Phosphorylation
T230 Phosphorylation Q04759 (PRKCQ)
T240 Phosphorylation
S246 Phosphorylation
S251 Phosphorylation
S252 Phosphorylation
S253 Phosphorylation
S254 Phosphorylation
S265 Phosphorylation Q04759 (PRKCQ)
T267 Phosphorylation

研究背景

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Heterodimer.

タンパク質ファミリー:

Belongs to the bZIP family. Fos subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

参考文献

1). Echinacoside alleviates glucocorticoid induce osteonecrosis of femoral head in rats through PI3K/AKT/FOXO1 pathway. Chemico-biological interactions, 2024 (PubMed: 38336255) [IF=5.1]
2). Glucocorticoids induce osteonecrosis of the femoral head in rats via PI3K/AKT/FOXO1 signaling pathway. PeerJ, 2022 (PubMed: 35529482) [IF=2.7]

Application: WB    Species: Mice    Sample: MC3T3-E1 cells

Figure 4 Dex inhibited the activities of PI3K/AKT signaling pathways in MC3T3-E1 cells. (A–B) Western blotting was performed to analyze the expression levels of PI3K, p-PI3K, AKT, p-AKT, FOXO1, and p-FOXO1 in MC3T3-E1 cells after Dex treatment (200 µM, 48 h). Con: control group. All data in vivo and in vitro experiments are triplicate biological replicates. * P < 0.05, ** P < 0.01, *** P < 0.0001.
3). Glucocorticoids induce osteonecrosis of the femoral head in rats via PI3K/AKT/FOXO1 signaling pathway. PeerJ, 2022 (PubMed: 35529482) [IF=2.7]

Application: WB    Species: Mice    Sample: MC3T3-E1 cells

Figure 4 Dex inhibited the activities of PI3K/AKT signaling pathways in MC3T3-E1 cells. (A–B) Western blotting was performed to analyze the expression levels of PI3K, p-PI3K, AKT, p-AKT, FOXO1, and p-FOXO1 in MC3T3-E1 cells after Dex treatment (200 µM, 48 h). Con: control group. All data in vivo and in vitro experiments are triplicate biological replicates. * P < 0.05, ** P < 0.01, *** P < 0.0001.

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