Phospho-Ras-GRF1 (Ser927) Antibody - #AF4414

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製品: Phospho-Ras-GRF1 (Ser927) Antibody
カタログ: AF4414
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Ras-GRF1 (Ser927)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 155kDa; 145kD(Calculated).
ユニプロット: Q13972
RRID: AB_2844479

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-Ras-GRF1 (Ser927) Antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at Ser927.
RRID:
AB_2844479
引用形式: Affinity Biosciences Cat# AF4414, RRID:AB_2844479.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CDC25; CDC25L; GNRP; GRF1; GRF55; Guanine nucleotide exchange factor; Guanine nucleotide releasing factor 55 kD; Guanine nucleotide releasing protein; guanine nucleotide-releasing factor 1; Guanine nucleotide-releasing protein; H GRF55; HGRF55; PP13187; Ras protein specific guanine nucleotide releasing factor 1; Ras specific guanine nucleotide releasing factor; Ras specific guanine nucleotide releasing factor CDC25 homolog; Ras specific nucleotide exchange factor CDC25; Ras-GRF1; Ras-specific guanine nucleotide-releasing factor 1; Ras-specific nucleotide exchange factor CDC25; RASGRF1; RGRF1_HUMAN;

免疫原

免疫原:
Uniprot:

Q13972(RGRF1_HUMAN) >>Visit HPA database.

遺伝子(ID):
RASGRF1(5923)
タンパク質配列:
MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEITSLLKDNERIQSTQTVAPNDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSMREESDIDQNQSDDGDTETSPTKSPTTPKSVKNKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSAIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

PTMs - Q13972 基板として

Site PTM Type Enzyme
S14 Phosphorylation
S287 Phosphorylation O96017 (CHEK2)
S481 Phosphorylation
S709 Phosphorylation
S730 Phosphorylation
S738 Phosphorylation
S750 Phosphorylation
S752 Phosphorylation
S758 Phosphorylation
S787 Phosphorylation
S927 Phosphorylation P17612 (PRKACA)
T1023 Phosphorylation
S1090 Phosphorylation
S1095 Phosphorylation
S1252 Phosphorylation

研究背景

機能:

Promotes the exchange of Ras-bound GDP by GTP.

PTMs:

Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.

Ubiquitinated and degraded following phosphorylation by PLK2.

Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity.

サブユニット構造:

Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability (By similarity).

タンパク質ファミリー:

The DH (DBL-homology) domain mediates interaction with RASGRF2.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

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