Phospho-MCL1 (Ser64) Antibody - #AF4457
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4457, RRID:AB_2844514.
折りたたみ／展開
Bcl 2 related protein EAT/mcl1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; BCL2 related; Bcl2-L-3; BCL2L3; EAT; Induced myeloid leukemia cell differentiation protein Mcl 1; Induced myeloid leukemia cell differentiation protein Mcl-1; MCL 1; MCL1; MCL1-ES; mcl1/EAT; MCL1_HUMAN; MCL1L; MCL1S; MGC104264; MGC1839; Myeloid Cell Leukemia 1; Myeloid cell leukemia ES; Myeloid cell leukemia sequence 1; Myeloid cell leukemia sequence 1 BCL2 related; Myeloid cell leukemia sequence 1 isoform 1; OTTHUMP00000032794; OTTHUMP00000032795; TM;
免疫原
- Q07820 MCL1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAGLAYLIR
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q07820 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Methylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| S64 | Phosphorylation | P45983 (MAPK8) , P24941 (CDK2) , P06493 (CDK1) | Uniprot |
| T68 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| T92 | Phosphorylation | P06493 (CDK1) , P27361 (MAPK3) , P24941 (CDK2) | Uniprot |
| S121 | Phosphorylation | P45983 (MAPK8) , P24941 (CDK2) , P53779 (MAPK10) | Uniprot |
| Y129 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| S159 | Phosphorylation | P49840 (GSK3A) , P49841 (GSK3B) | Uniprot |
| S162 | Phosphorylation | Uniprot | |
| T163 | Phosphorylation | P24941 (CDK2) , P53779 (MAPK10) , P27361 (MAPK3) , P45983 (MAPK8) , P28482 (MAPK1) | Uniprot |
| K194 | Ubiquitination | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| Y347 | Phosphorylation | Uniprot |
研究背景
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.
Membrane>Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus>Nucleoplasm.
Note: Cytoplasmic, associated with mitochondria.
Interacts with HIF3A (via C-terminus domain) (By similarity). Interacts with BAD, BOK, BIK and BMF (By similarity). Interacts with PMAIP1. Interacts with BBC3 (By similarity). Isoform 1 interacts with BAX, BAK1 and TPT1. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptotic BCL2-related proteins. Interacts with RTL10/BOP. Interacts with BCL2L11; may sequester BCL2L11 to prevent its pro-apoptotic activity. Interacts with GIMAP5 and HSPA8/HSC70; the interaction between HSPA8 and MCL1 is impaired in the absence of GIMAP5 (By similarity).
Belongs to the Bcl-2 family.
研究領域
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
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