Phospho-FOXM1 (Ser35) Antibody - #AF4458

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製品: Phospho-FOXM1 (Ser35) Antibody
カタログ: AF4458
タンパク質の説明: Rabbit polyclonal antibody to Phospho-FOXM1 (Ser35)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Rabbit, Dog
分子量: 84kD, 100-130kD; 84kD(Calculated).
ユニプロット: Q08050
RRID: AB_2844515

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Rabbit(82%), Dog(91%)
クローナリティ:
Polyclonal
特異性:
Phospho-FOXM1 (Ser35) Antibody detects endogenous levels of FOXM1 only when phosphorylated at Ser35.
RRID:
AB_2844515
引用形式: Affinity Biosciences Cat# AF4458, RRID:AB_2844515.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FKHL16; Forkhead box M1; Forkhead box protein M1; forkhead like 16; Forkhead-related protein FKHL16; FOX M1; Foxm1; FOXM1_HUMAN; FOXM1B; Hepatocyte nuclear factor 3 forkhead homolog 11; HFH-11; HFH11; HNF-3/fork-head homolog 11; HNF3; INS1; M phase phosphoprotein 2; M-phase phosphoprotein 2; MPHOSPH2; MPM-2 reactive phosphoprotein 2; MPP2; PIG29; TGT3; Transcription factor Trident; Trident; WIN; Winged-helix factor from INS-1 cells; Winged-helix factor from INS1 cells;

免疫原

免疫原:
Uniprot:

Q08050(FOXM1_HUMAN) >>Visit HPA database.

遺伝子(ID):
FOXM1(2305)
発現特異性:
Q08050 FOXM1_HUMAN:

Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.

タンパク質配列:
MKTSPRRPLILKRRRLPLPVQNAPSETSEEEPKRSPAQQESNQAEASKEVAESNSCKFPAGIKIINHPTMPNTQVVAIPNNANIHSIITALTAKGKESGSSGPNKFILISCGGAPTQPPGLRPQTQTSYDAKRTEVTLETLGPKPAARDVNLPRPPGALCEQKRETCADGEAAGCTINNSLSNIQWLRKMSSDGLGSRSIKQEMEEKENCHLEQRQVKVEEPSRPSASWQNSVSERPPYSYMAMIQFAINSTERKRMTLKDIYTWIEDHFPYFKHIAKPGWKNSIRHNLSLHDMFVRETSANGKVSFWTIHPSANRYLTLDQVFKPLDPGSPQLPEHLESQQKRPNPELRRNMTIKTELPLGARRKMKPLLPRVSSYLVPIQFPVNQSLVLQPSVKVPLPLAASLMSSELARHSKRVRIAPKVLLAEEGIAPLSSAGPGKEEKLLFGEGFSPLLPVQTIKEEEIQPGEEMPHLARPIKVESPPLEEWPSPAPSFKEESSHSWEDSSQSPTPRPKKSYSGLRSPTRCVSEMLVIQHRERRERSRSRRKQHLLPPCVDEPELLFSEGPSTSRWAAELPFPADSSDPASQLSYSQEVGGPFKTPIKETLPISSTPSKSVLPRTPESWRLTPPAKVGGLDFSPVQTSQGASDPLPDPLGLMDLSTTPLQSAPPLESPQRLLSSEPLDLISVPFGNSSPSDIDVPKPGSPEPQVSGLAANRSLTEGLVLDTMNDSLSKILLDISFPGLDEDPLGPDNINWSQFIPELQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Dog
91
Rabbit
82
Horse
73
Bovine
67
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08050 基板として

Site PTM Type Enzyme
S4 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
S35 Phosphorylation P11802 (CDK4) , Q00534 (CDK6)
S41 Phosphorylation
K57 Ubiquitination
K63 Acetylation
K189 Ubiquitination
S191 Phosphorylation
K201 Sumoylation
K218 Sumoylation
S251 Phosphorylation P06493 (CDK1)
K278 Methylation
K278 Ubiquitination
K282 Methylation
S331 Phosphorylation
T354 Phosphorylation
K356 Sumoylation
T357 Phosphorylation
S376 Phosphorylation O96017 (CHEK2)
K422 Acetylation
K422 Sumoylation
K422 Ubiquitination
K440 Acetylation
K440 Sumoylation
K440 Ubiquitination
S451 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
K460 Sumoylation
K478 Sumoylation
S481 Phosphorylation
S489 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
K495 Sumoylation
S501 Phosphorylation
S508 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
T510 Phosphorylation P11802 (CDK4) , Q00534 (CDK6)
Y517 Phosphorylation
S522 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
T524 Phosphorylation
T600 Phosphorylation P11802 (CDK4) , P06493 (CDK1) , Q00534 (CDK6)
K603 Acetylation
K603 Sumoylation
T611 Phosphorylation P11802 (CDK4) , P06493 (CDK1) , Q00534 (CDK6)
K614 Acetylation
T620 Phosphorylation Q00534 (CDK6) , P11802 (CDK4) , P06493 (CDK1)
S623 Phosphorylation
T627 Phosphorylation P11802 (CDK4) , Q00534 (CDK6)
S638 Phosphorylation
S672 Phosphorylation
S692 Phosphorylation
S693 Phosphorylation
S704 Phosphorylation Q00534 (CDK6) , P11802 (CDK4)
S717 Phosphorylation
T719 Phosphorylation
S730 Phosphorylation P53350 (PLK1)
S739 Phosphorylation P53350 (PLK1)

研究背景

機能:

Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response.

PTMs:

Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis.

タンパク質ファミリー:

Within the protein there is a domain which acts as a transcriptional activator. Insertion of a splicing sequence within it inactivates this transcriptional activity, as it is the case for isoform 4.

研究領域

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

参考文献

1). TRIM6 promotes colorectal cancer cells proliferation and response to thiostrepton by TIS21/FoxM1. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2020 (PubMed: 31992359) [IF=11.3]

Application: WB    Species: Human    Sample: Sw620 cells

Fig. 6 TIS21/FoxM1 was essential for TRIM6-inhibited CRC cell proliferation and cell cycle progression. a, Sw620 cells were transfected with plasmids expressing TRIM6, TIS21 or vector. Overexpression of TRIM6 or TIS21 was confirmed by western blotting. B-F, Sw620 cells were divided into four groups: Vector (cells transfected with vector), TRIM6 (cells transfected with plasmid expressing TRIM6), TIS21 (cells transfected with plasmid expressing TIS21) and TRIM6 + TIS21 (cells transfected with plasmid expressing TRIM6 and plasmid expressing TIS21). CCK-8 (b), BrdU (c), flow cytometry analyses (d) and western blotting (e, f) were performed to determine the effects of TRIM6 and TIS21 on the proliferation, cell cycle distribution and relative protein expression, respectively. F, HCT-8 cells were transfected with plasmids expressing FoxM1 or vector. Overexpression of TRIM6 or TIS21 was confirmed by western blotting. G-J, HCT-8 cells were divided into four groups: Vector+shNC (cells treated with vector and control shRNA), Vector+shTRIM6 (cells treated with vector and TRIM6 shRNA), FoxM1 + shNC (cells treated with plamid expressing FoxM1 and control shRNA) and FoxM1 + shTRIM6 (cells treated with plamid expressing FoxM1 and TRIM6 shRNA). CCK-8 (g), BrdU (h), flow cytometry analyses (i) and western blotting (j) were performed to determine the effects of FoxM1 overexpression and TRIM6 knockdown on the proliferation, cell cycle distribution and relative protein expression, respectively. *P < 0.05, **P < 0.05, ***P < 0.001

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