Phospho-SLP76 (Tyr145) Antibody - #AF4462
| 製品: | Phospho-SLP76 (Tyr145) Antibody |
| カタログ: | AF4462 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-SLP76 (Tyr145) |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 65~85kD; 60kD(Calculated). |
| ユニプロット: | Q13094 |
| RRID: | AB_2844519 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4462, RRID:AB_2844519.
折りたたみ／展開
76 kDa tyrosine phosphoprotein; CG8697; LCP 2; LCP2; LCP2_HUMAN; Lymphocyte cytosolic protein 2; SH2 domain containing leukocyte protein 76 KD; SH2 domain containing leukocyte protein of 76kD; SH2 domain containing leukocyte protein of 76kDa; SH2 domain-containing leukocyte protein of 76 kDa; SLP 76; SLP 76 tyrosine phosphoprotein; SLP-76; SLP-76 tyrosine phosphoprotein; SLP76; SLP76 tyrosine phosphoprotein;
免疫原
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
- Q13094 LCP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALRNVPFRSEVLGWDPDSLADYFKKLNYKDCEKAVKKYHIDGARFLNLTENDIQKFPKLRVPILSKLSQEINKNEERRSIFTRKPQVPRFPEETESHEEDNGGWSSFEEDDYESPNDDQDGEDDGDYESPNEEEEAPVEDDADYEPPPSNDEEALQNSILPAKPFPNSNSMYIDRPPSGKTPQQPPVPPQRPMAALPPPPAGRNHSPLPPPQTNHEEPSRSRNHKTAKLPAPSIDRSTKPPLDRSLAPFDREPFTLGKKPPFSDKPSIPAGRSLGEHLPKIQKPPLPPTTERHERSSPLPGKKPPVPKHGWGPDRRENDEDDVHQRPLPQPALLPMSSNTFPSRSTKPSPMNPLPSSHMPGAFSESNSSFPQSASLPPYFSQGPSNRPPIRAEGRNFPLPLPNKPRPPSPAEEENSLNEEWYVSYITRPEAEAALRKINQDGTFLVRDSSKKTTTNPYVLMVLYKDKVYNIQIRYQKESQVYLLGTGLRGKEDFLSVSDIIDYFRKMPLLLIDGKNRGSRYQCTLTHAAGYP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13094 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y29 | Phosphorylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| Y113 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK) | Uniprot |
| Y128 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) , P42681 (TXK) , P43405 (SYK) | Uniprot |
| S130 | Phosphorylation | Uniprot | |
| Y145 | Phosphorylation | P43403 (ZAP70) , P06239 (LCK) , P42681 (TXK) | Uniprot |
| S169 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| Y173 | Phosphorylation | Q08881 (ITK) | Uniprot |
| S207 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| R273 | Methylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| R392 | Methylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot | |
| Y423 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) | Uniprot |
| S425 | Phosphorylation | Uniprot | |
| Y426 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) | Uniprot |
| T428 | Phosphorylation | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| T454 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| T456 | Phosphorylation | Uniprot | |
| Y459 | Phosphorylation | Uniprot | |
| Y465 | Phosphorylation | Uniprot | |
| Y470 | Phosphorylation | Uniprot | |
| Y476 | Phosphorylation | Uniprot | |
| Y483 | Phosphorylation | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| Y522 | Phosphorylation | Uniprot | |
| Y532 | Phosphorylation | Uniprot |
研究背景
Involved in T-cell antigen receptor mediated signaling.
Phosphorylated after T-cell receptor activation by ZAP70, ITK and TXK, which leads to the up-regulation of Th1 preferred cytokine IL-2. SYK-dependent phosphorylation is required for recruitment of PI3K signaling components.
Cytoplasm.
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines.
Interacts with SLA. Interacts with CBLB (By similarity). Interacts with GRB2. Interacts with SHB. Interacts with PRAM1. Interacts (via SH2 domain) with CD6 (via tyrosine phosphorylated C-terminus). Interacts with FYB1 and the phosphorylated form of FYB2.
The SH2 domain mediates interaction with phosphorylated CD6 (PubMed:16914752). The SH2 domain mediates interaction with SHB (PubMed:12084069).
研究領域
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
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