製品: Phospho-PAR4 (Thr163) Antibody
カタログ: AF4485
タンパク質の説明: Rabbit polyclonal antibody to Phospho-PAR4 (Thr163)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Rabbit, Chicken, Xenopus
分子量: 43kDa; 37kD(Calculated).
ユニプロット: Q96IZ0
RRID: AB_2844536

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(92%), Rabbit(100%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
Phospho-PAR4 (Thr163) Antibody detects endogenous levels of PAR4 only when phosphorylated at Thr163.
RRID:
AB_2844536
引用形式: Affinity Biosciences Cat# AF4485, RRID:AB_2844536.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

2310001G03Rik; PAR 4; PAR-4; Pawr; PAWR_HUMAN; PRKC Apoptosis WT1 Regulator; PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4; Transcriptional repressor Par-4-like protein PAWR; Transcriptional repressor PAR4; WT1 Interacting Protein;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q96IZ0 PAWR_HUMAN:

Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.

タンパク質配列:
MATGGYRTSSGLGGSTTDFLEEWKAKREKMRAKQNPPGPAPPGGGSSDAAGKPPAGALGTPAAAAANELNNNLPGGAPAAPAVPGPGGVNCAVGSAMLTRAAPGPRRSEDEPPAASASAAPPPQRDEEEPDGVPEKGKSSGPSARKGKGQIEKRKLREKRRSTGVVNIPAAECLDEYEDDEAGQKERKREDAITQQNTIQNEAVNLLDPGSSYLLQEPPRTVSGRYKSTTSVSEEDVSSRYSRTDRSGFPRYNRDANVSGTLVSSSTLEKKIEDLEKEVVRERQENLRLVRLMQDKEEMIGKLKEEIDLLNRDLDDIEDENEQLKQENKTLLKVVGQLTR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
92
Horse
0
Bovine
0
Sheep
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96IZ0 基板として

Site PTM Type Enzyme
T8 Phosphorylation
S15 Phosphorylation
S95 Phosphorylation
T99 Phosphorylation
S108 Phosphorylation
K146 Methylation
S162 Phosphorylation
T163 Phosphorylation
C173 S-Nitrosylation
Y177 Phosphorylation
S223 Phosphorylation
Y226 Phosphorylation
K227 Methylation
K227 Ubiquitination
S228 Phosphorylation
T229 Phosphorylation
T230 Phosphorylation
S231 Phosphorylation P68400 (CSNK2A1)
S233 Phosphorylation
Y241 Phosphorylation
S242 Phosphorylation
S259 Phosphorylation
T261 Phosphorylation
T267 Phosphorylation
K277 Ubiquitination
K296 Acetylation
K296 Ubiquitination
K302 Acetylation
K304 Ubiquitination
K329 Ubiquitination
K333 Ubiquitination

研究背景

機能:

Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.

PTMs:

Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.

サブユニット構造:

Homooligomer. Interacts (via the C-terminal region) with WT1. Interacts with THAP1. Interacts with AATF. Interacts with BACE1. Interacts with SPSB1 (via B30.2/SPRY domain); this interaction is direct and occurs in association with the Elongin BC complex. Interacts with SPSB2 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex. Interacts with SPSB4 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex. Component of a ternary complex composed of SQSTM1 and PRKCZ. Interacts with actin (By similarity).

タンパク質ファミリー:

The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.

The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells (By similarity).

The B30.2/SPRY domain-binding motif mediates recognition by proteins containing a B30.2/SPRY domain.

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