Phospho-Mnk1 (Thr250/Thr255) Antibody - #AF4488
| 製品: | Phospho-Mnk1 (Thr250/Thr255) Antibody |
| カタログ: | AF4488 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Mnk1 (Thr250/Thr255) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 50kDa; 51kD(Calculated). |
| ユニプロット: | Q9BUB5 |
| RRID: | AB_2844539 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4488, RRID:AB_2844539.
折りたたみ／展開
MAP kinase interacting kinase 1; MAP kinase interacting serine/threonine kinase 1; MAP kinase signal integrating kinase 1; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine-protein kinase 1; MAPK signal integrating kinase 1; MITOGEN-ACTIVATED PROTEIN KINASE-INTERACTING SERINE/THREONINE KINASE 1; mknk1; MKNK1_HUMAN; MNK 1; Mnk1;
免疫原
- Q9BUB5 MKNK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQGNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRDVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRQLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BUB5 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S39 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| Y60 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T250 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| T255 | Phosphorylation | Q16539 (MAPK14) , P28482 (MAPK1) | Uniprot |
| K332 | Ubiquitination | Uniprot | |
| K338 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| T385 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| S393 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| R455 | Methylation | Uniprot | |
| R459 | Methylation | Uniprot | |
| S460 | Phosphorylation | Uniprot |
PTMs - Q9BUB5 酵素として
| Substrate | Site | Source |
|---|---|---|
| O43597 (SPRY2) | S112 | Uniprot |
| O43597 (SPRY2) | S121 | Uniprot |
| P06730 (EIF4E) | S209 | Uniprot |
| P09651 (HNRNPA1) | S192 | Uniprot |
| P09651 (HNRNPA1) | S362 | Uniprot |
| P09651 (HNRNPA1) | S363 | Uniprot |
| P09651 (HNRNPA1) | S364 | Uniprot |
| P23246 (SFPQ) | S8 | Uniprot |
| P23246 (SFPQ) | S283 | Uniprot |
| P47712 (PLA2G4A) | S727 | Uniprot |
| Q9NP80 (PNPLA8) | S511 | Uniprot |
研究背景
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Dual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase. MAPK3/ERK1 is one of the kinases which activate MKNK1/MNK1. Phosphorylation by PAK2 leads to a reduced phosphorylation of EIF4G1.
Cytoplasm.
Cytoplasm. Nucleus.
Ubiquitous.
Interacts with the C-terminal regions of EIF4G1 and EIF4G2. Also binds to dephosphorylated ERK1 and ERK2, and to the p38 kinases.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
研究領域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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