Phospho-CDCP1 (Tyr707) Antibody - #AF4492
製品: | Phospho-CDCP1 (Tyr707) Antibody |
カタログ: | AF4492 |
タンパク質の説明: | Rabbit polyclonal antibody to Phospho-CDCP1 (Tyr707) |
アプリケーション: | WB |
反応性: | Human, Mouse, Rat |
予測: | Pig, Horse, Sheep |
分子量: | 60~70kD(cleaved),90kDa,140kD; 93kD(Calculated). |
ユニプロット: | Q9H5V8 |
RRID: | AB_2844543 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4492, RRID:AB_2844543.
折りたたみ/展開
9030022E12Rik; AA409659; CD 318; CD_antigen=CD318; CD318; CD318 antigen; CDCP 1; Cdcp1; CDCP1_HUMAN; CUB domain containing protein 1; CUB domain-containing protein 1; E030027H19Rik; FLJ13772; FLJ22375; FLJ22969; Membrane glycoprotein gp140; MGC31813; OTTHUMP00000164120; OTTHUMP00000209568; RGD1305578; SIMA 135; SIMA135; Subtractive immunization M plus HEp3 associated 135 kDa protein; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases; TRASK;
免疫原
Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
- Q9H5V8 CDCP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAPSFQQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDLTVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGPAVGIYNDNINTEMPRQPKKFQKGRKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMGVCPPSPPTICSRAPTAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H5V8 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S9 | Phosphorylation | Uniprot | |
K71 | Ubiquitination | Uniprot | |
S142 | Phosphorylation | Uniprot | |
S164 | Phosphorylation | Uniprot | |
T170 | Phosphorylation | Uniprot | |
S184 | Phosphorylation | Uniprot | |
S210 | Phosphorylation | Uniprot | |
Y283 | Phosphorylation | Uniprot | |
Y284 | Phosphorylation | Uniprot | |
K296 | Ubiquitination | Uniprot | |
K343 | Ubiquitination | Uniprot | |
S458 | Phosphorylation | Uniprot | |
K542 | Ubiquitination | Uniprot | |
K554 | Ubiquitination | Uniprot | |
S575 | Phosphorylation | Uniprot | |
S579 | Phosphorylation | Uniprot | |
T660 | Phosphorylation | Uniprot | |
K700 | Ubiquitination | Uniprot | |
Y707 | Phosphorylation | Uniprot | |
S731 | Phosphorylation | Uniprot | |
Y734 | Phosphorylation | Q05397 (PTK2) , P12931 (SRC) | Uniprot |
Y743 | Phosphorylation | P12931 (SRC) | Uniprot |
Y762 | Phosphorylation | P12931 (SRC) | Uniprot |
S775 | Phosphorylation | Uniprot | |
K787 | Ubiquitination | Uniprot | |
S797 | Phosphorylation | Uniprot | |
S801 | Phosphorylation | Uniprot | |
S803 | Phosphorylation | Uniprot | |
Y806 | Phosphorylation | Uniprot | |
T807 | Phosphorylation | Uniprot | |
S809 | Phosphorylation | Uniprot |
研究背景
May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.
Cell membrane>Single-pass membrane protein.
Note: Shedding may also lead to a soluble peptide.
Secreted.
Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
Interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1. Also interacts with SRC and PRKCG/protein kinase C gamma.
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