製品: Phospho-DAPP1/BAM32 (Tyr139) Antibody
カタログ: AF4502
タンパク質の説明: Rabbit polyclonal antibody to Phospho-DAPP1/BAM32 (Tyr139)
アプリケーション: WB IHC
反応性: Human, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 29kDa; 32kD(Calculated).
ユニプロット: Q9UN19
RRID: AB_2844553

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
予測:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-DAPP1/BAM32 (Tyr139) Antibody detects endogenous levels of DAPP1/BAM32 only when phosphorylated at Tyr139.
RRID:
AB_2844553
引用形式: Affinity Biosciences Cat# AF4502, RRID:AB_2844553.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

B cell adapter molecule of 32 kDa; B lymphocyte adapter protein Bam32; B-cell adapter molecule of 32 kDa; BAM32; DAPP1; DAPP1_HUMAN; DKFZp667E0716; Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; Dual adaptor of phosphotyrosine and 3 phosphoinositides; hDAPP1;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9UN19 DAPP1_HUMAN:

Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.

タンパク質配列:
MGRAELLEGKMSTQDPSDLWSRSDGEAELLQDLGWYHGNLTRHAAEALLLSNGCDGSYLLRDSNETTGLYSLSVRAKDSVKHFHVEYTGYSFKFGFNEFSSLKDFVKHFANQPLIGSETGTLMVLKHPYPRKVEEPSIYESVRVHTAMQTGRTEDDLVPTAPSLGTKEGYLTKQGGLVKTWKTRWFTLHRNELKYFKDQMSPEPIRILDLTECSAVQFDYSQERVNCFCLVFPFRTFYLCAKTGVEADEWIKILRWKLSQIRKQLNQGEGTIRSRSFIFK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UN19 基板として

Site PTM Type Enzyme
K132 Ubiquitination
S137 Phosphorylation
Y139 Phosphorylation P06239 (LCK) , P07948 (LYN) , P12931 (SRC)
S141 Phosphorylation
K167 Ubiquitination
K173 Ubiquitination
K182 Acetylation
K263 Ubiquitination
S274 Phosphorylation
S276 Phosphorylation

研究背景

機能:

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.

PTMs:

Phosphorylated on tyrosine residues.

細胞の位置付け:

Cytoplasm. Membrane>Peripheral membrane protein.
Note: Membrane-associated after cell stimulation leading to its translocation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.

サブユニット構造:

Interacts with PtdIns(3,4,5)P3 and PLCG2. In vitro, interacts with PtdIns(3,4)P2.

研究領域

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

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