MRIP Antibody - #AF4608
| 製品: | MRIP Antibody |
| カタログ: | AF4608 |
| タンパク質の説明: | Rabbit polyclonal antibody to MRIP |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 115kDa; 117kD(Calculated). |
| ユニプロット: | Q6WCQ1 |
| RRID: | AB_2844561 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4608, RRID:AB_2844561.
折りたたみ／展開
9530046C02; AA536749; AI647711; C76423; KIAA0864; M-RIP; MGC67316; mKIAA0864; Mprip; MPRIP_HUMAN; Myosin phosphatase Rho-interacting protein; p116 Rho-interacting protein; p116Rip; Rho-interacting protein 3; Rhoip3; RIP3; RP23-180B18.4;
免疫原
- Q6WCQ1 MPRIP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSAAKENPCRKFQANIFNKSKCQNCFKPRESHLLNDEDLTQAKPIYGGWLLLAPDGTDFDNPVHRSRKWQRRFFILYEHGLLRYALDEMPTTLPQGTINMNQCTDVVDGEGRTGQKFSLCILTPEKEHFIRAETKEIVSGWLEMLMVYPRTNKQNQKKKRKVEPPTPQEPGPAKVAVTSSSSSSSSSSSIPSAEKVPTTKSTLWQEEMRTKDQPDGSSLSPAQSPSQSQPPAASSLREPGLESKEEESAMSSDRMDCGRKVRVESGYFSLEKTKQDLKAEEQQLPPPLSPPSPSTPNHRRSQVIEKFEALDIEKAEHMETNAVGPSPSSDTRQGRSEKRAFPRKRDFTNEAPPAPLPDASASPLSPHRRAKSLDRRSTEPSVTPDLLNFKKGWLTKQYEDGQWKKHWFVLADQSLRYYRDSVAEEAADLDGEIDLSACYDVTEYPVQRNYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVHPTTAPDVTSSLPEEKNKSSCSFETCPRPTEKQEAELGEPDPEQKRSRARERRREGRSKTFDWAEFRPIQQALAQERVGGVGPADTHEPLRPEAEPGELERERARRREERRKRFGMLDATDGPGTEDAALRMEVDRSPGLPMSDLKTHNVHVEIEQRWHQVETTPLREEKQVPIAPVHLSSEDGGDRLSTHELTSLLEKELEQSQKEASDLLEQNRLLQDQLRVALGREQSAREGYVLQATCERGFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATISAIEAMKNAHREEMERELEKSQRSQISSVNSDVEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQALEAERQALRQCQRENQELNAHNQELNNRLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLRVKESEIQYLKQEISSLKDELQTALRDKKYASDKYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPDSATVSGYDIMKSKSNPDFLKKDRSCVTRQLRNIRSKSVIEQVSWDT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q6WCQ1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K5 | Ubiquitination | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S192 | Phosphorylation | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| Y267 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| T295 | Phosphorylation | Uniprot | |
| S301 | Phosphorylation | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| T348 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| T378 | Phosphorylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| K390 | Ubiquitination | Uniprot | |
| K396 | Ubiquitination | Uniprot | |
| Y450 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| S501 | Phosphorylation | Uniprot | |
| S504 | Phosphorylation | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| T542 | Phosphorylation | Uniprot | |
| T602 | Phosphorylation | Uniprot | |
| S619 | Phosphorylation | Uniprot | |
| S625 | Phosphorylation | Uniprot | |
| T646 | Phosphorylation | Uniprot | |
| S662 | Phosphorylation | Uniprot | |
| S663 | Phosphorylation | Uniprot | |
| K681 | Ubiquitination | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| Y718 | Phosphorylation | Uniprot | |
| S800 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| S891 | Phosphorylation | Uniprot | |
| Y915 | Phosphorylation | Uniprot | |
| K917 | Ubiquitination | Uniprot | |
| S921 | Phosphorylation | Uniprot | |
| Y945 | Phosphorylation | Uniprot | |
| T946 | Phosphorylation | Uniprot | |
| T970 | Phosphorylation | Uniprot | |
| K976 | Ubiquitination | Uniprot | |
| S977 | Phosphorylation | Uniprot | |
| S980 | Phosphorylation | Uniprot | |
| S984 | Phosphorylation | Uniprot | |
| Y986 | Phosphorylation | Uniprot | |
| K990 | Methylation | Uniprot | |
| S991 | Phosphorylation | Uniprot | |
| K992 | Methylation | Uniprot | |
| S993 | Phosphorylation | Uniprot | |
| S1014 | Phosphorylation | Uniprot | |
| S1016 | Phosphorylation | Uniprot |
研究背景
Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.
Cytoplasm>Cytoskeleton.
Note: Colocalizes with F-actin.
Binds F-actin through its N-terminus. Interacts with MYZAP (By similarity). Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.