ASPP2 Antibody - #AF4619
| 製品: | ASPP2 Antibody |
| カタログ: | AF4619 |
| タンパク質の説明: | Rabbit polyclonal antibody to ASPP2 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 126kDa; 126kD(Calculated). |
| ユニプロット: | Q13625 |
| RRID: | AB_2844566 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4619, RRID:AB_2844566.
折りたたみ／展開
53BP2; Apoptosis stimulating of p53 protein 2; Apoptosis stimulating protein of p53 2; Apoptosis-stimulating of p53 protein 2; ASPP2; ASPP2_HUMAN; BBP; Bcl2 binding protein; Bcl2-binding protein; NY REN 51 antigen; p53 binding protein 2; p53-binding protein 2; p53BP2; PPP1R13A; Renal carcinoma antigen NY-REN-51; Tp53bp2; Tumor protein p53 binding protein 2; Tumor suppressor p53 binding protein 2; Tumor suppressor p53-binding protein 2;
免疫原
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
- Q13625 ASPP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMPMFLTVYLSNNEQHFTEVPVTPETICRDVVDLCKEPGESDCHLAEVWCGSERPVADNERMFDVLQRFGSQRNEVRFFLRHERPPGRDIVSGPRSQDPSLKRNGVKVPGEYRRKENGVNSPRMDLTLAELQEMASRQQQQIEAQQQLLATKEQRLKFLKQQDQRQQQQVAEQEKLKRLKEIAENQEAKLKKVRALKGHVEQKRLSNGKLVEEIEQMNNLFQQKQRELVLAVSKVEELTRQLEMLKNGRIDSHHDNQSAVAELDRLYKELQLRNKLNQEQNAKLQQQRECLNKRNSEVAVMDKRVNELRDRLWKKKAALQQKENLPVSSDGNLPQQAASAPSRVAAVGPYIQSSTMPRMPSRPELLVKPALPDGSLVIQASEGPMKIQTLPNMRSGAASQTKGSKIHPVGPDWSPSNADLFPSQGSASVPQSTGNALDQVDDGEVPLREKEKKVRPFSMFDAVDQSNAPPSFGTLRKNQSSEDILRDAQVANKNVAKVPPPVPTKPKQINLPYFGQTNQPPSDIKPDGSSQQLSTVVPSMGTKPKPAGQQPRVLLSPSIPSVGQDQTLSPGSKQESPPAAAVRPFTPQPSKDTLLPPFRKPQTVAASSIYSMYTQQQAPGKNFQQAVQSALTKTHTRGPHFSSVYGKPVIAAAQNQQQHPENIYSNSQGKPGSPEPETEPVSSVQENHENERIPRPLSPTKLLPFLSNPYRNQSDADLEALRKKLSNAPRPLKKRSSITEPEGPNGPNIQKLLYQRTTIAAMETISVPSYPSKSASVTASSESPVEIQNPYLHVEPEKEVVSLVPESLSPEDVGNASTENSDMPAPSPGLDYEPEGVPDNSPNLQNNPEEPNPEAPHVLDVYLEEYPPYPPPPYPSGEPEGPGEDSVSMRPPEITGQVSLPPGKRTNLRKTGSERIAHGMRVKFNPLALLLDSSLEGEFDLVQRIIYEVDDPSLPNDEGITALHNAVCAGHTEIVKFLVQFGVNVNAADSDGWTPLHCAASCNNVQVCKFLVESGAAVFAMTYSDMQTAADKCEEMEEGYTQCSQFLYGVQEKMGIMNKGVIYALWDYEPQNDDELPMKEGDCMTIIHREDEDEIEWWWARLNDKEGYVPRNLLGLYPRIKPRQRSLA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13625 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S71 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| R265 | Methylation | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K275 | Ubiquitination | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| Y350 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| T355 | Phosphorylation | Uniprot | |
| S361 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| T474 | Phosphorylation | Uniprot | |
| S480 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| T586 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T603 | Phosphorylation | Uniprot | |
| S607 | Phosphorylation | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| Y613 | Phosphorylation | Uniprot | |
| Y645 | Phosphorylation | Uniprot | |
| Y664 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S667 | Phosphorylation | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| T700 | Phosphorylation | Uniprot | |
| Y710 | Phosphorylation | Uniprot | |
| S714 | Phosphorylation | Uniprot | |
| K724 | Ubiquitination | Uniprot | |
| S736 | Phosphorylation | Uniprot | |
| S737 | Phosphorylation | Uniprot | |
| T739 | Phosphorylation | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| Y791 | Phosphorylation | Uniprot | |
| S827 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| Y869 | Phosphorylation | Uniprot | |
| Y874 | Phosphorylation | Uniprot | |
| Y947 | Phosphorylation | Uniprot |
研究背景
Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions with proteins such as TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
Cytoplasm>Perinuclear region. Nucleus.
Note: Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
Interacts with P53/TP53; the interaction promotes pro-apototic activity. Interacts with BCL2. Interacts with protein phosphatase 1. Interacts with RELA NF-kappa-B subunit. This interaction probably prevents the activation of apoptosis, possibly by preventing its interaction with TP53. Interacts with APC2 and APPBP1. Interacts with DDX42 (via the C-terminus); the interaction is not inhibited by TP53BP2 ubiquitination and is independent of p53/TP53.
The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
Belongs to the ASPP family.
研究領域
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
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