製品: BACH1/BRIP1 Antibody
カタログ: AF4789
タンパク質の説明: Rabbit polyclonal antibody to BACH1/BRIP1
アプリケーション: WB
反応性: Human, Mouse
予測: Pig, Bovine, Dog, Chicken
分子量: 145kDa; 141kD(Calculated).
ユニプロット: Q9BX63
RRID: AB_2844609

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Dog(92%), Chicken(91%)
クローナリティ:
Polyclonal
特異性:
BACH1/BRIP1 Antibody detects endogenous levels of total BACH1/BRIP1.
RRID:
AB_2844609
引用形式: Affinity Biosciences Cat# AF4789, RRID:AB_2844609.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ATP dependent RNA helicase BRIP1; ATP-dependent RNA helicase BRIP1; BACH 1; BRAC 1 Associated C Terminal Helicase 1; BRCA 1 Interacting Protein 1; BRCA1 binding helicase like protein BACH1; BRCA1 interacting protein C terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1/BRCA2 associated helicase 1; BRIP 1; BRIP1; FANCJ; FANCJ_HUMAN; Fanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ;

免疫原

免疫原:

A synthesized peptide derived from human BACH1/BRIP1, corresponding to a region within C-terminal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q9BX63 FANCJ_HUMAN:

Ubiquitously expressed, with highest levels in testis.

タンパク質配列:
MSSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSGKPADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTSRHFNYPSTPPSERNGTSSTCQDSPEKTTLAAKLSAKKQASIYRDENDDFQVEKKRIRPLETTQQIRKRHCFGTEVHNLDAKVDSGKTVKLNSPLEKINSFSPQKPPGHCSRCCCSTKQGNSQESSNTIKKDHTGKSKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRDHTCVHPEVVGNFNRNEKCMELLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELVSLGKKLKACPYYTARELIQDADIIFCPYNYLLDAQIRESMDLNLKEQVVILDEAHNIEDCARESASYSVTEVQLRFARDELDSMVNNNIRKKDHEPLRAVCCSLINWLEANAEYLVERDYESACKIWSGNEMLLTLHKMGITTATFPILQGHFSAVLQKEEKISPIYGKEEAREVPVISASTQIMLKGLFMVLDYLFRQNSRFADDYKIAIQQTYSWTNQIDISDKNGLLVLPKNKKRSRQKTAVHVLNFWCLNPAVAFSDINGKVQTIVLTSGTLSPMKSFSSELGVTFTIQLEANHIIKNSQVWVGTIGSGPKGRNLCATFQNTETFEFQDEVGALLLSVCQTVSQGILCFLPSYKLLEKLKERWLSTGLWHNLELVKTVIVEPQGGEKTNFDELLQVYYDAIKYKGEKDGALLVAVCRGKVSEGLDFSDDNARAVITIGIPFPNVKDLQVELKRQYNDHHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHRNDWGALILVDDRFRNNPSRYISGLSKWVRQQIQHHSTFESALESLAEFSKKHQKVLNVSIKDRTNIQDNESTLEVTSLKYSTSPYLLEAASHLSPENFVEDEAKICVQELQCPKIITKNSPLPSSIISRKEKNDPVFLEEAGKAEKIVISRSTSPTFNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPFTDKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRDFETEAEDESIYFTPELYDPEDTDEEKNDLAETDRGNRLANNSDCILAKDLFEIRTIKEVDSAREVKAEDCIDTKLNGILHIEESKIDDIDGNVKTTWINELELGKTHEIEIKNFKPSPSKNKGMFPGFK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
92
Chicken
91
Zebrafish
43
Horse
0
Sheep
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.

PTMs:

Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.

Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling.

細胞の位置付け:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitously expressed, with highest levels in testis.

タンパク質ファミリー:

4Fe-4S iron-sulfur-binding is required for helicase activity.

Belongs to the DEAD box helicase family. DEAH subfamily.

研究領域

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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