CYLD Antibody - #AF4796
製品: | CYLD Antibody |
カタログ: | AF4796 |
タンパク質の説明: | Rabbit polyclonal antibody to CYLD |
アプリケーション: | WB |
反応性: | Human |
予測: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 107kDa; 107kD(Calculated). |
ユニプロット: | Q9NQC7 |
RRID: | AB_2844612 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4796, RRID:AB_2844612.
折りたたみ/展開
BRSS; CDMT; Cyld; CYLD gene; CYLD_HUMAN; CYLD1; CYLDI; cylindromatosis (turban tumor syndrome); cylindromatosis 1; Deubiquitinating enzyme CYLD; EAC; HSPC057; KIAA0849; MFT; MFT1; Probable ubiquitin carboxyl terminal hydrolase CYLD; SBS; TEM; turban tumor syndrome; Ubiquitin carboxyl-terminal hydrolase CYLD; ubiquitin specific peptidase like 2; ubiquitin thioesterase CYLD; Ubiquitin thiolesterase CYLD; Ubiquitin-specific processing protease CYLD; Ubiquitin-specific-processing protease CYLD; USPL2;
免疫原
Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney.
- Q9NQC7 CYLD_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSGLWSQEKVTSPYWEERIFYLLLQECSVTDKQTQKLLKVPKGSIGQYIQDRSVGHSRIPSAKGKKNQIGLKILEQPHAVLFVDEKDVVEINEKFTELLLAITNCEERFSLFKNRNRLSKGLQIDVGCPVKVQLRSGEEKFPGVVRFRGPLLAERTVSGIFFGVELLEEGRGQGFTDGVYQGKQLFQCDEDCGVFVALDKLELIEDDDTALESDYAGPGDTMQVELPPLEINSRVSLKVGETIESGTVIFCDVLPGKESLGYFVGVDMDNPIGNWDGRFDGVQLCSFACVESTILLHINDIIPALSESVTQERRPPKLAFMSRGVGDKGSSSHNKPKATGSTSDPGNRNRSELFYTLNGSSVDSQPQSKSKNTWYIDEVAEDPAKSLTEISTDFDRSSPPLQPPPVNSLTTENRFHSLPFSLTKMPNTNGSIGHSPLSLSAQSVMEELNTAPVQESPPLAMPPGNSHGLEVGSLAEVKENPPFYGVIRWIGQPPGLNEVLAGLELEDECAGCTDGTFRGTRYFTCALKKALFVKLKSCRPDSRFASLQPVSNQIERCNSLAFGGYLSEVVEENTPPKMEKEGLEIMIGKKKGIQGHYNSCYLDSTLFCLFAFSSVLDTVLLRPKEKNDVEYYSETQELLRTEIVNPLRIYGYVCATKIMKLRKILEKVEAASGFTSEEKDPEEFLNILFHHILRVEPLLKIRSAGQKVQDCYFYQIFMEKNEKVGVPTIQQLLEWSFINSNLKFAEAPSCLIIQMPRFGKDFKLFKKIFPSLELNITDLLEDTPRQCRICGGLAMYECRECYDDPDISAGKIKQFCKTCNTQVHLHPKRLNHKYNPVSLPKDLPDWDWRHGCIPCQNMELFAVLCIETSHYVAFVKYGKDDSAWLFFDSMADRDGGQNGFNIPQVTPCPEVGEYLKMSLEDLHSLDSRRIQGCARRLLCDAYMCMYQSPTMSLYK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NQC7 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S3 | Phosphorylation | Uniprot | |
K10 | Ubiquitination | Uniprot | |
T12 | Phosphorylation | Uniprot | |
S13 | Phosphorylation | Uniprot | |
Y15 | Phosphorylation | Uniprot | |
Y22 | Phosphorylation | Uniprot | |
S29 | Phosphorylation | Uniprot | |
T31 | Phosphorylation | Uniprot | |
T35 | Phosphorylation | Uniprot | |
K40 | Ubiquitination | Uniprot | |
K43 | Ubiquitination | Uniprot | |
S45 | Phosphorylation | Uniprot | |
K73 | Ubiquitination | Uniprot | |
K114 | Ubiquitination | Uniprot | |
K121 | Ubiquitination | Uniprot | |
K132 | Ubiquitination | Uniprot | |
K141 | Ubiquitination | Uniprot | |
S287 | Phosphorylation | Uniprot | |
K318 | Ubiquitination | Uniprot | |
T343 | Phosphorylation | Uniprot | |
S344 | Phosphorylation | Uniprot | |
Y356 | Phosphorylation | Uniprot | |
S362 | Phosphorylation | Q13554 (CAMK2B) | Uniprot |
S369 | Phosphorylation | Uniprot | |
K372 | Ubiquitination | Uniprot | |
Y376 | Phosphorylation | Uniprot | |
S387 | Phosphorylation | Uniprot | |
T389 | Phosphorylation | Uniprot | |
T393 | Phosphorylation | Uniprot | |
S399 | Phosphorylation | Uniprot | |
S409 | Phosphorylation | Uniprot | |
S418 | Phosphorylation | O14920 (IKBKB) , O15111 (CHUK) , Q14164 (IKBKE) , Q13554 (CAMK2B) | Uniprot |
S422 | Phosphorylation | O14920 (IKBKB) , O15111 (CHUK) | Uniprot |
T424 | Phosphorylation | Uniprot | |
S432 | Phosphorylation | O14920 (IKBKB) , O15111 (CHUK) | Uniprot |
S436 | Phosphorylation | O14920 (IKBKB) , O15111 (CHUK) | Uniprot |
S439 | Phosphorylation | O14920 (IKBKB) , O15111 (CHUK) | Uniprot |
S441 | Phosphorylation | O15111 (CHUK) , O14920 (IKBKB) | Uniprot |
S444 | Phosphorylation | O14920 (IKBKB) , O15111 (CHUK) | Uniprot |
Y523 | Phosphorylation | Uniprot | |
K530 | Ubiquitination | Uniprot | |
S547 | Phosphorylation | Uniprot | |
S552 | Phosphorylation | Uniprot | |
S560 | Phosphorylation | Uniprot | |
K627 | Ubiquitination | Uniprot | |
K668 | Ubiquitination | Uniprot | |
K701 | Ubiquitination | Uniprot | |
K761 | Ubiquitination | Uniprot | |
K768 | Ubiquitination | Uniprot | |
S772 | Phosphorylation | Q13554 (CAMK2B) | Uniprot |
K818 | Ubiquitination | Uniprot | |
K829 | Ubiquitination | Uniprot | |
K834 | Ubiquitination | Uniprot | |
K842 | Ubiquitination | Uniprot |
研究背景
Deubiquitinase that specifically cleaves 'Lys-63'- and linear 'Met-1'-linked polyubiquitin chains and is involved in NF-kappa-B activation and TNF-alpha-induced necroptosis. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells (By similarity). Negatively regulates TNFRSF11A signaling and osteoclastogenesis (By similarity). Involved in the regulation of ciliogenesis, allowing ciliary basal bodies to migrate and dock to the plasma membrane; this process does not depend on NF-kappa-B activation (By similarity). Ability to remove linear ('Met-1'-linked) polyubiquitin chains regulates innate immunity and TNF-alpha-induced necroptosis: recruited to the LUBAC complex via interaction with SPATA2 and restricts linear polyubiquitin formation on target proteins. Regulates innate immunity by restricting linear polyubiquitin formation on RIPK2 in response to NOD2 stimulation. Involved in TNF-alpha-induced necroptosis by removing linear ('Met-1'-linked) polyubiquitin chains from RIPK1, thereby regulating the kinase activity of RIPK1 (By similarity). Removes 'Lys-63' linked polyubiquitin chain of MAP3K7, which inhibits phosphorylation and blocks downstream activation of the JNK-p38 kinase cascades.
Ubiquitinated. Polyubiquitinated in hepatocytes treated with palmitic acid. Ubiquitination is mediated by E3 ligase TRIM47 and leads to proteasomal degradation.
Phosphorylated on several serine residues by IKKA and/or IKKB in response to immune stimuli. Phosphorylation requires IKBKG. Phosphorylation abolishes TRAF2 deubiquitination, interferes with the activation of Jun kinases, and strongly reduces CD40-dependent gene activation by NF-kappa-B.
Cytoplasm. Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Detected at the microtubule cytoskeleton during interphase. Detected at the midbody during telophase. During metaphase, it remains localized to the centrosome but is also present along the spindle (PubMed:25134987).
Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney.
Interacts (via CAP-Gly domain) with IKBKG/NEMO (via proline-rich C-terminal region). Interacts with TRAF2 and TRIP. Interacts with PLK1, DVL1, DVL3, MAVS, TBK1, IKKE and DDX58. Interacts (via CAP-Gly domain) with microtubules. Interacts with HDAC6 and BCL3. Interacts with MAP3K7 (By similarity). Identified in a complex with TRAF6 and SQSTM1 (By similarity). Interacts with CEP350. Interacts with RNF31; the interaction is indirect and is mediated via SPATA2. Interacts with SPATA2 (via the PUB domain); the interaction is direct and recruits CYLD to the LUBAC complex, thereby regulating TNF-alpha-induced necroptosis.
Belongs to the peptidase C19 family.
研究領域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.