Phospho-SOX9 (Ser181) Antibody - #AF3330
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3330, RRID:AB_2834745.
折りたたみ／展開
campomelic dysplasia autosomal sex reversal; CMD 1; CMD1; CMPD 1; CMPD1; SOX 9; Sox9; SOX9_HUMAN; SRA 1; SRA1; SRXX2; SRXY10; SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal; SRY (sex determining region Y) box 9; SRY (sex determining region Y)-box 9; SRY (sex-determining region Y)-box 9 protein; SRY related HMG box gene 9; Transcription factor SOX 9; Transcription factor SOX-9; transcription factor SOX9;
免疫原
- P48436 SOX9_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQAPPPPPQQPPQAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P48436 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T11 | Phosphorylation | Uniprot | |
| S30 | Phosphorylation | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| K61 | Acetylation | Uniprot | |
| S64 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| K68 | Ubiquitination | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| S181 | Phosphorylation | P17612 (PRKACA) , Q13237 (PRKG2) , Q13464 (ROCK1) | Uniprot |
| T196 | Phosphorylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| T236 | Phosphorylation | Uniprot | |
| T239 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K398 | Acetylation | Uniprot | |
| S414 | Phosphorylation | Uniprot | |
| Y442 | Phosphorylation | Uniprot |
研究背景
Transcriptional regulator that plays a role in chondrocytes differentiation and skeletal development. Binds to the COL2A1 promoter and activates COL2A1 expression, as part of a complex with ZNF219 (By similarity).
Ubiquitinated. Ubiquitination leads to proteasomal degradation and is negatively regulated by DDRGK1.
Nucleus.
Interacts (via C-terminus) with ZNF219. Interacts with DDRGK1.
研究領域
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
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