Phospho-RapGEF1 (Thr1071) Antibody - #AF4469
| 製品: | Phospho-RapGEF1 (Thr1071) Antibody |
| カタログ: | AF4469 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-RapGEF1 (Thr1071) |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 121kDa; 121kD(Calculated). |
| ユニプロット: | Q13905 |
| RRID: | AB_2844637 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4469, RRID:AB_2844637.
折りたたみ／展開
C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2 (specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTHUMP00000064558; Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; Rap guanine nucleotide exchange factor 1; RAPGEF 1; RAPGEF1; RPGF1_HUMAN;
免疫原
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
- Q13905 RPGF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q13905 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| Y329 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| Y390 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| Y504 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) , P08631 (HCK) | Uniprot |
| Y561 | Phosphorylation | Uniprot | |
| Y570 | Phosphorylation | Uniprot | |
| T572 | Phosphorylation | Uniprot | |
| Y579 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| K683 | Ubiquitination | Uniprot | |
| Y715 | Phosphorylation | Uniprot | |
| Y723 | Phosphorylation | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| T819 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| K869 | Ubiquitination | Uniprot | |
| T950 | Phosphorylation | Uniprot | |
| S951 | Phosphorylation | Uniprot | |
| S965 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| T1067 | Phosphorylation | Uniprot | |
| T1071 | Phosphorylation | Uniprot |
研究背景
Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor.
Early endosome.
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
Interacts with CRK via its SH3-binding sites.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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