Phospho-NudC (Ser326) Antibody - #AF4504
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4504, RRID:AB_2844642.
折りたたみ／展開
HNUDC; MNUDC; MNUDC protein; NPD011; Nuclear distribution C homolog; Nuclear distribution gene C (A.nidulans) homolog; Nuclear distribution gene C homolog; Nuclear distribution gene C homolog (A. nidulans); Nuclear distribution protein C homolog; Nuclear migration protein nudC; nudC; NudC nuclear distribution protein; NUDC_HUMAN; OTTHUMP00000004405; SIG 92; SIG92;
免疫原
Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart.
- Q9Y266 NUDC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGGEQEEERFDGMLLAMAQQHEGGVQELVNTFFSFLRRKTDFFIGGEEGMAEKLITQTFSHHNQLAQKTRREKRARQEAERREKAERAARLAKEAKSETSGPQIKELTDEEAERLQLEIDQKKDAENHEAQLKNGSLDSPGKQDTEEDEEEDEKDKGKLKPNLGNGADLPNYRWTQTLSELDLAVPFCVNFRLKGKDMVVDIQRRHLRVGLKGQPAIIDGELYNEVKVEESSWLIEDGKVVTVHLEKINKMEWWSRLVSSDPEINTKKINPENSKLSDLDSETRSMVEKMMYDQRQKSMGLPTSDEQKKQEILKKFMDQHPEMDFSKAKFN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y266 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K39 | Acetylation | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| T40 | Phosphorylation | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| T58 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| K68 | Acetylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K93 | Acetylation | Uniprot | |
| K96 | Sumoylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| T108 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S139 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T145 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K212 | Ubiquitination | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| K239 | Acetylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| T266 | Phosphorylation | Uniprot | |
| K267 | Acetylation | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| S274 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K275 | Ubiquitination | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K327 | Methylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K329 | Methylation | Uniprot |
研究背景
Plays a role in neurogenesis and neuronal migration (By similarity). Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.
Reversibly phosphorylated on serine residues during the M phase of the cell cycle. Phosphorylation on Ser-274 and Ser-326 is necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Phosphorylated by PLK and other kinases.
Cytoplasm>Cytoskeleton. Nucleus.
Note: In a filamentous pattern adjacent to the nucleus of migrating cerebellar granule cells. Colocalizes with tubulin and dynein and with the microtubule organizing center. Distributed throughout the cytoplasm of non-migrating cells. A small proportion is nuclear, in a punctate pattern.
Ubiquitous. Highly expressed in fetal liver, kidney, lung and brain. Highly expressed in adult pancreas, kidney, skeletal muscle, liver, lung, placenta, prostate, brain and heart.
Interacts with PAFAH1B1 (By similarity). Interacts with PLK1. Part of a complex containing PLK1, NUDC, dynein and dynactin. Interacts with DCDC1.
Belongs to the nudC family.
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