Phospho-Rabphilin 3A (Ser424) Antibody - #AF4505
| 製品: | Phospho-Rabphilin 3A (Ser424) Antibody |
| カタログ: | AF4505 |
| タンパク質の説明: | Rabbit polyclonal antibody to Phospho-Rabphilin 3A (Ser424) |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 75~90kDa; 77kD(Calculated). |
| ユニプロット: | Q9Y2J0 |
| RRID: | AB_2844643 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4505, RRID:AB_2844643.
折りたたみ／展開
Exophilin 1; Exophilin-1; Exophilin1; KIAA0985; Rabphilin 3A homolog; Rabphilin-3A; Rabphilin3A; RP3A; RP3A_HUMAN; Rph 3a; Rph3a;
免疫原
- Q9Y2J0 RP3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTDTVFSNSSNRWMYPSDRPLQSNDKEQLQAGWSVHPGGQPDRQRKQEELTDEEKEIINRVIARAEKMEEMEQERIGRLVDRLENMRKNVAGDGVNRCILCGEQLGMLGSACVVCEDCKKNVCTKCGVETNNRLHSVWLCKICIEQREVWKRSGAWFFKGFPKQVLPQPMPIKKTKPQQPVSEPAAPEQPAPEPKHPARAPARGDSEDRRGPGQKTGPDPASAPGRGNYGPPVRRASEARMSSSSRDSESWDHSGGAGDSSRSPAGLRRANSVQASRPAPGSVQSPAPPQPGQPGTPGGSRPGPGPAGRFPDQKPEVAPSDPGTTAPPREERTGGVGGYPAVGAREDRMSHPSGPYSQASAAAPQPAAARQPPPPEEEEEEANSYDSDEATTLGALEFSLLYDQDNSSLQCTIIKAKGLKPMDSNGLADPYVKLHLLPGASKSNKLRTKTLRNTRNPIWNETLVYHGITDEDMQRKTLRISVCDEDKFGHNEFIGETRFSLKKLKPNQRKNFNICLERVIPMKRAGTTGSARGMALYEEEQVERVGDIEERGKILVSLMYSTQQGGLIVGIIRCVHLAAMDANGYSDPFVKLWLKPDMGKKAKHKTQIKKKTLNPEFNEEFFYDIKHSDLAKKSLDISVWDYDIGKSNDYIGGCQLGISAKGERLKHWYECLKNKDKKIERWHQLQNENHVSSD
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y2J0 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T51 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| T333 | Phosphorylation | Uniprot | |
| Y356 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| T477 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot |
研究背景
Plays an essential role in docking and fusion steps of regulated exocytosis (By similarity). At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release (By similarity). In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Plays also a role in the exocytosis of arginine vasopressin hormone (By similarity).
Ubiquitinated. Deubiquitinated by CAND1 to prevent its degradation.
Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle membrane. Cell projection>Dendritic spine. Cell junction>Synapse>Postsynaptic cell membrane. Membrane>Peripheral membrane protein.
Interacts with RAB3B, RAB3C, RAB3D, RAB8A, RAB27A and RAB27B (By similarity). Interacts with RAB3A; this interaction recruits RPH3A to synaptic vesicules. Interacts (via C2B domain) with SNAP25 (By similarity). Interacts with deubiquitinating enzyme CAND1; this interaction results in the deubiquitination of RPH3A (By similarity). Interacts with GRIN2A and DLG4; this ternary complex regulates NMDA receptor composition at postsynaptic membranes (By similarity). Interacts with SNCA.
Binds calcium via the C2 domains. The calcium-bound C2 domains mediate interactions with phospholipid bilayers (By similarity).
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