Occludin Antibody - #AF4605
(4)
(2)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4605, RRID:AB_2844646.
折りたたみ／展開
BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Ocln; OCLN_HUMAN; Tight junction protein occludin;
免疫原
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
- Q16625 OCLN_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q16625 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| S45 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| Y287 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S302 | Phosphorylation | Uniprot | |
| T305 | Phosphorylation | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| Y315 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| Y325 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| Y337 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| Y342 | Phosphorylation | Uniprot | |
| K343 | Ubiquitination | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| T345 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| Y368 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| T376 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| T393 | Phosphorylation | Uniprot | |
| Y398 | Phosphorylation | P12931 (SRC) | Uniprot |
| T400 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| Y402 | Phosphorylation | P12931 (SRC) | Uniprot |
| T403 | Phosphorylation | Q02156 (PRKCE) , P24723 (PRKCH) , Q05513 (PRKCZ) | Uniprot |
| T404 | Phosphorylation | Q02156 (PRKCE) , Q05513 (PRKCZ) , P24723 (PRKCH) , P68400 (CSNK2A1) , P67870 (CSNK2B) | Uniprot |
| S408 | Phosphorylation | P67870 (CSNK2B) , P68400 (CSNK2A1) | Uniprot |
| T424 | Phosphorylation | Q02156 (PRKCE) , Q05513 (PRKCZ) | Uniprot |
| T438 | Phosphorylation | Q02156 (PRKCE) , Q05513 (PRKCZ) | Uniprot |
| Y443 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| Y467 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| Y474 | Phosphorylation | Uniprot | |
| Y481 | Phosphorylation | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| S490 | Phosphorylation | P05771 (PRKCB) | Uniprot |
研究背景
May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.
Cell membrane>Multi-pass membrane protein. Cell junction>Tight junction.
Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.
Interacts with TJP1/ZO1. Interacts with VAPA. Interacts with CLDN1, CLDN6, CLDN9, CLDN11, CLDN12 and CLDN17.
The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.
Belongs to the ELL/occludin family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
参考文献
Application: WB Species: rat Sample: brain
Application: WB Species: mouse Sample: brain
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