APAF1 Antibody - #AF4633
| 製品: | APAF1 Antibody |
| カタログ: | AF4633 |
| タンパク質の説明: | Rabbit polyclonal antibody to APAF1 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| 分子量: | 139kDa; 142kD(Calculated). |
| ユニプロット: | O14727 |
| RRID: | AB_2844656 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4633, RRID:AB_2844656.
折りたたみ／展開
APAF 1; Apaf-1; APAF_HUMAN; Apaf1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor 1; Apoptotic protease activating factor; Apoptotic protease-activating factor 1; CED 4; CED4; KIAA0413;
免疫原
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
- O14727 APAF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDAKARNCLLQHREALEKDIKTSYIMDHMISDGFLTISEEEKVRNEPTQQQRAAMLIKMILKKDNDSYVSFYNALLHEGYKDLAALLHDGIPVVSSSSGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDILQEFVNKSLLFCDRNGKSFRYYLHDLQVDFLTEKNCSQLQDLHKKIITQFQRYHQPHTLSPDQEDCMYWYNFLAYHMASAKMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDCAVSENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYQQAKLQAKQEVDNGMLYLEWINKKNITNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSTDDRFIATCSVDKKVKIWNSMTGELVHTYDEHSEQVNCCHFTNSSHHLLLATGSSDCFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDKLLASCSADGTLKLWDATSANERKSINVKQFFLNLEDPQEDMEVIVKCCSWSADGARIMVAAKNKIFLFDIHTSGLLGEIHTGHHSTIQYCDFSPQNHLAVVALSQYCVELWNTDSRSKVADCRGHLSWVHGVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSAVMLKQEVDVVFQENEVMVLAVDHIRRLQLINGRTGQIDYLTEAQVSCCCLSPHLQYIAFGDENGAIEILELVNNRIFQSRFQHKKTVWHIQFTADEKTLISSSDDAEIQVWNWQLDKCIFLRGHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITGNKEKDFVCHQGTVLSCDISHDATKFSSTSADKTAKIWSFDLLLPLHELRGHNGCVRCSAFSVDSTLLATGDDNGEIRIWNVSNGELLHLCAPLSEEGAATHGGWVTDLCFSPDGKMLISAGGYIKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILQTLE
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14727 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y24 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K144 | Ubiquitination | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| T204 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| S268 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| S272 | Phosphorylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| Y278 | Phosphorylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| S369 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| Y382 | Phosphorylation | Uniprot | |
| Y383 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| S506 | Phosphorylation | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| K601 | Ubiquitination | Uniprot | |
| K637 | Ubiquitination | Uniprot | |
| K643 | Acetylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| K654 | Acetylation | Uniprot | |
| K733 | Ubiquitination | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K768 | Ubiquitination | Uniprot | |
| K818 | Ubiquitination | Uniprot | |
| S901 | Phosphorylation | Uniprot | |
| T905 | Phosphorylation | Uniprot | |
| K916 | Ubiquitination | Uniprot | |
| K1048 | Ubiquitination | Uniprot | |
| K1077 | Ubiquitination | Uniprot | |
| K1079 | Ubiquitination | Uniprot | |
| K1099 | Ubiquitination | Uniprot | |
| T1103 | Phosphorylation | Uniprot | |
| K1107 | Ubiquitination | Uniprot | |
| T1108 | Phosphorylation | Uniprot | |
| K1222 | Ubiquitination | Uniprot |
研究背景
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Cytoplasm.
Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Monomer. Oligomerizes to a heptameric ring, known as the apoptosome, upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). Interacts with CIAO2A.
The CARD domain mediates interaction with APIP.
The monomeric form is autoinhibited in a closed conformation through a bound ADP at the nucleotide binding site. Exchange of ADP for ATP and binding of cytochrome c trigger a large conformational change where the first WD repeat region swings out, allowing the NB-ARC domain to rotate and expose the contact areas for oligomerization (By similarity).
研究領域
· Cellular Processes > Cell growth and death > p53 signaling pathway. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)
· Cellular Processes > Cell growth and death > Apoptosis - multiple species. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).
· Human Diseases > Neurodegenerative diseases > Huntington's disease.
· Human Diseases > Infectious diseases: Bacterial > Legionellosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
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