LAT Antibody - #AF4680
製品: | LAT Antibody |
カタログ: | AF4680 |
タンパク質の説明: | Rabbit polyclonal antibody to LAT |
アプリケーション: | WB |
反応性: | Human |
予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
分子量: | 28kDa; 28kD(Calculated). |
ユニプロット: | O43561 |
RRID: | AB_2844686 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4680, RRID:AB_2844686.
折りたたみ/展開
36 kDa phospho tyrosine adapter protein; 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; LAT 1; lat; LAT_HUMAN; LAT1; Linker for activation of T cells; Linker for activation of T cells family member 1; linker for activation of T cells, transmembrane adaptor; Linker for activation of T-cells family member 1; p36 38; p36-38; pp36;
免疫原
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
- O43561 LAT_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEEAILVPCVLGLLLLPILAMLMALCVHCHRLPGSYDSTSSDSLYPRGIQFKRPHTVAPWPPAYPPVTSYPPLSQPDLLPIPRSPQPLGGSHRTPSSRRDSDGANSVASYENEGASGIRGAQAGWGVWGPSWTRLTPVSLPPEPACEDADEDEDDYHNPGYLVVLPDSTPATSTAAPSAPALSTPGIRDSAFSMESIDDYVNVPESGESAEASLDGSREYVNVSQELHPGAAKTEPAALSSQEAEEVEEEGAPDYENLQELN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43561 基板として
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S35 | Phosphorylation | Uniprot | |
Y36 | Phosphorylation | Uniprot | |
S38 | Phosphorylation | Uniprot | |
T39 | Phosphorylation | Uniprot | |
S40 | Phosphorylation | Uniprot | |
S41 | Phosphorylation | Uniprot | |
S43 | Phosphorylation | Uniprot | |
Y45 | Phosphorylation | Uniprot | |
K52 | Ubiquitination | Uniprot | |
Y64 | Phosphorylation | Uniprot | |
T68 | Phosphorylation | Uniprot | |
Y70 | Phosphorylation | Uniprot | |
S74 | Phosphorylation | Uniprot | |
S84 | Phosphorylation | Uniprot | |
S91 | Phosphorylation | Uniprot | |
T94 | Phosphorylation | Uniprot | |
S96 | Phosphorylation | Uniprot | |
S97 | Phosphorylation | Uniprot | |
S101 | Phosphorylation | Uniprot | |
S106 | Phosphorylation | Uniprot | |
S109 | Phosphorylation | Uniprot | |
Y110 | Phosphorylation | Uniprot | |
S131 | Phosphorylation | Uniprot | |
Y156 | Phosphorylation | P43403 (ZAP70) | Uniprot |
Y161 | Phosphorylation | P43403 (ZAP70) | Uniprot |
S183 | Phosphorylation | Uniprot | |
T184 | Phosphorylation | P27361 (MAPK3) , P45983 (MAPK8) | Uniprot |
Y200 | Phosphorylation | P06239 (LCK) , P43403 (ZAP70) , Q08881 (ITK) , P06241 (FYN) | Uniprot |
S206 | Phosphorylation | Uniprot | |
S213 | Phosphorylation | Uniprot | |
S217 | Phosphorylation | Uniprot | |
Y220 | Phosphorylation | P06241 (FYN) , P43403 (ZAP70) , P06239 (LCK) , Q08881 (ITK) | Uniprot |
S224 | Phosphorylation | Uniprot | |
S240 | Phosphorylation | Uniprot | |
S241 | Phosphorylation | Uniprot | |
Y255 | Phosphorylation | P43403 (ZAP70) | Uniprot |
研究背景
Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes.
Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
Cell membrane>Single-pass type III membrane protein.
Note: Present in lipid rafts.
Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK (By similarity). Interacts with FCGR1A. Interacts with GRB2, PLCG1 and THEMIS upon TCR activation in thymocytes (By similarity).
研究領域
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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