AS160 Antibody - #AF4682

(1)  
製品: AS160 Antibody
カタログ: AF4682
タンパク質の説明: Rabbit polyclonal antibody to AS160
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 147kDa; 147kD(Calculated).
ユニプロット: O60343
RRID: AB_2844687

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WB Handbook
IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
クローナリティ:
Polyclonal
特異性:
AS160 Antibody detects endogenous levels of total AS160.
RRID:
AB_2844687
引用形式: Affinity Biosciences Cat# AF4682, RRID:AB_2844687.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Acrg embryonic lethality (mouse) minimal region ortholog; Acrg embryonic lethality minimal region ortholog; Acrg embryonic lethality mouse minimal region ortholog; Akt substrate of 160 kDa; AS 160; AS160; BUB2; CDC16; KIAA0603; NIDDM5; TBC (Tre 2 BUB2 CDC16) domain containing protein; TBC Tre 2 BUB2 CDC16 domain containing protein; TBC1 D4; TBC1 domain family member 4; Tbc1d4; TBCD4_HUMAN; Tre-2;

免疫原

免疫原:
Uniprot:

O60343(TBCD4_HUMAN) >>Visit HPA database.

遺伝子(ID):
TBC1D4(9882)
発現特異性:
O60343 TBCD4_HUMAN:

Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.

タンパク質配列:
MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEIRQDTASESSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60343 基板として

Site PTM Type Enzyme
M1 Acetylation
S44 Phosphorylation
S66 Phosphorylation
K68 Ubiquitination
S102 Phosphorylation
K228 Ubiquitination
K238 Ubiquitination
S262 Phosphorylation
T274 Phosphorylation
S285 Phosphorylation
S304 Phosphorylation
S314 Phosphorylation
S317 Phosphorylation
S318 Phosphorylation O00141 (SGK1) , P31749 (AKT1)
T320 Phosphorylation
S330 Phosphorylation
S341 Phosphorylation Q15418 (RPS6KA1)
S344 Phosphorylation
S349 Phosphorylation
S351 Phosphorylation
T356 Phosphorylation
S370 Phosphorylation
K383 Ubiquitination
K477 Acetylation
S485 Phosphorylation O14757 (CHEK1)
S486 Phosphorylation
T488 Phosphorylation
S551 Phosphorylation
K555 Ubiquitination
K560 Ubiquitination
S566 Phosphorylation
T568 Phosphorylation O00141 (SGK1)
S569 Phosphorylation
S570 Phosphorylation
S576 Phosphorylation
S588 Phosphorylation O14757 (CHEK1) , P31749 (AKT1) , O00141 (SGK1)
S591 Phosphorylation
S595 Phosphorylation
S597 Phosphorylation
S600 Phosphorylation
S609 Phosphorylation
T613 Phosphorylation
S617 Phosphorylation
S621 Phosphorylation
S633 Phosphorylation
T642 Phosphorylation O00141 (SGK1) , P31749 (AKT1)
S644 Phosphorylation
S648 Phosphorylation
S649 Phosphorylation
S666 Phosphorylation
S672 Phosphorylation
S673 Phosphorylation
S678 Phosphorylation
T701 Phosphorylation
S704 Phosphorylation
S707 Phosphorylation
T749 Phosphorylation
S750 Phosphorylation
S751 Phosphorylation O00141 (SGK1) , Q15418 (RPS6KA1)
T752 Phosphorylation
S754 Phosphorylation
S757 Phosphorylation
S759 Phosphorylation
T763 Phosphorylation
S764 Phosphorylation
T766 Phosphorylation
S782 Phosphorylation
S787 Phosphorylation
S789 Phosphorylation
S806 Phosphorylation
S809 Phosphorylation
Y1191 Phosphorylation
S1207 Phosphorylation
S1246 Phosphorylation
K1255 Ubiquitination
K1267 Ubiquitination

研究背景

機能:

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

PTMs:

Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.

細胞の位置付け:

Cytoplasm.
Note: Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.

研究領域

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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