RAD52 Antibody - #AF4731
| 製品: | RAD52 Antibody |
| カタログ: | AF4731 |
| タンパク質の説明: | Rabbit polyclonal antibody to RAD52 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 46kDa; 46kD(Calculated). |
| ユニプロット: | P43351 |
| RRID: | AB_2844730 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4731, RRID:AB_2844730.
折りたたみ／展開
DNA repair protein RAD52; DNA repair protein RAD52; DNA repair protein RAD52 homolog; RAD 52; Rad52; RAD52 homolog (S. cerevisiae); RAD52 homolog (S. cerevisiae); RAD52 homolog; RAD52_HUMAN; Recombination protein RAD52; Recombination protein RAD52; Rhabdomyosarcoma antigen MU RMS 40.23;
免疫原
- P43351 RAD52_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGTEEAILGGRDSHPAAGGGSVLCFGQCQYTAEEYQAIQKALRQRLGPEYISSRMAGGGQKVCYIEGHRVINLANEMFGYNGWAHSITQQNVDFVDLNNGKFYVGVCAFVRVQLKDGSYHEDVGYGVSEGLKSKALSLEKARKEAVTDGLKRALRSFGNALGNCILDKDYLRSLNKLPRQLPLEVDLTKAKRQDLEPSVEEARYNSCRPNMALGHPQLQQVTSPSRPSHAVIPADQDCSSRSLSSSAVESEATHQRKLRQKQLQQQFRERMEKQQVRVSTPSAEKSEAAPPAPPVTHSTPVTVSEPLLEKDFLAGVTQELIKTLEDNSEKWAVTPDAGDGVVKPSSRADPAQTSDTLALNNQMVTQNRTPHSVCHQKPQAKSGSWDLQTYSADQRTTGNWESHRKSQDMKKRKYDPS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P43351 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| Y104 | Phosphorylation | P00519 (ABL1) , A0A173G4P4 (Abl fusion) , A9UF07 (BCR/ABL fusion) | Uniprot |
| S129 | Phosphorylation | Uniprot | |
| K133 | Acetylation | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| Y171 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| K177 | Acetylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| K190 | Acetylation | Uniprot | |
| K192 | Acetylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| Y205 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| T223 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| K262 | Acetylation | Uniprot | |
| K274 | Acetylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| K286 | Acetylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| K323 | Acetylation | Uniprot | |
| T335 | Phosphorylation | Uniprot | |
| K344 | Acetylation | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| K406 | Acetylation | Uniprot | |
| K411 | Sumoylation | Uniprot | |
| K412 | Acetylation | Uniprot | |
| K412 | Sumoylation | Uniprot | |
| K414 | Acetylation | Uniprot | |
| K414 | Sumoylation | Uniprot |
研究背景
Involved in double-stranded break repair. Plays a central role in genetic recombination and DNA repair by promoting the annealing of complementary single-stranded DNA and by stimulation of the RAD51 recombinase.
Phosphorylated upon DNA damage by ABL1, and probably by ATM or ATR.
Nucleus.
The full-length protein forms heptameric rings. Interacts with ABL1. Interacts with RPA2; the interaction is direct and associates RAD52 with the RPA complex.
Belongs to the RAD52 family.
研究領域
· Genetic Information Processing > Replication and repair > Homologous recombination.
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