Phospho-BMX (Tyr566) Antibody - #AF3341
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3341, RRID:AB_2834756.
折りたたみ／展開
Bmx; BMX non receptor tyrosine kinase; BMX_HUMAN; BONE MARROW KINASE, X-LINKED; Bone marrow tyrosine kinase gene in chromosome X protein; Cytoplasmic tyrosine protein kinase BMX; Cytoplasmic tyrosine-protein kinase BMX; Epithelial and endothelial tyrosine kinase; ETK; NKT38; NTK 38; NTK38; Protein tyrosine kinase BMX; PSCTK 2; PSCTK 3; PSCTK2; PSCTK3;
免疫原
Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines.
- P51813 BMX_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDTKSILEELLLKRSQQKKKMSPNNYKERLFVLTKTNLSYYEYDKMKRGSRKGSIEIKKIRCVEKVNLEEQTPVERQYPFQIVYKDGLLYVYASNEESRSQWLKALQKEIRGNPHLLVKYHSGFFVDGKFLCCQQSCKAAPGCTLWEAYANLHTAVNEEKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKKNYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPDWWQVRKLKSSSSSEDVASSNQKERNVNHTTSKISWEFPESSSSEEEENLDDYDWFAGNISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVKHYHVHTNAENKLYLAENYCFDSIPKLIHYHQHNSAGMITRLRHPVSTKANKVPDSVSLGNGIWELKREEITLLKELGSGQFGVVQLGKWKGQYDVAVKMIKEGSMSEDEFFQEAQTMMKLSHPKLVKFYGVCSKEYPIYIVTEYISNGCLLNYLRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDRDLCVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVWAFGILMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIYQIMYSCWHELPEKRPTFQQLLSSIEPLREKDKH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P51813 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y40 | Phosphorylation | Q05397 (PTK2) | Uniprot |
| K172 | Acetylation | Uniprot | |
| K181 | Acetylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| Y216 | Phosphorylation | Q08881 (ITK) , Q06187 (BTK) , P51813 (BMX) , P42680 (TEC) | Uniprot |
| Y224 | Phosphorylation | Q08881 (ITK) , P42680 (TEC) , P51813 (BMX) , Q06187 (BTK) | Uniprot |
| S226 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| Y330 | Phosphorylation | Uniprot | |
| K337 | Acetylation | Uniprot | |
| K343 | Acetylation | Uniprot | |
| K347 | Acetylation | Uniprot | |
| Y365 | Phosphorylation | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| K395 | Acetylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| S425 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| Y566 | Phosphorylation | P12931 (SRC) , P51813 (BMX) | Uniprot |
| S568 | Phosphorylation | Uniprot |
PTMs - P51813 酵素として
| Substrate | Site | Source |
|---|---|---|
| P08581 (MET) | Y1235 | Uniprot |
| P11362 (FGFR1) | Y653 | Uniprot |
| P11362 (FGFR1) | Y654 | Uniprot |
| P51813 (BMX) | Y216 | Uniprot |
| P51813 (BMX) | Y224 | Uniprot |
| P51813 (BMX) | Y566 | Uniprot |
| P56945 (BCAR1) | Y12 | Uniprot |
| Q05397 (PTK2) | Y194 | Uniprot |
| Q05397 (PTK2) | Y441 | Uniprot |
| Q05397 (PTK2) | Y576 | Uniprot |
| Q05397 (PTK2) | Y577 | Uniprot |
| Q05397 (PTK2) | Y742 | Uniprot |
| Q05397 (PTK2) | Y861 | Uniprot |
| Q05397 (PTK2) | Y898 | Uniprot |
| Q05397 (PTK2) | Y925 | Uniprot |
| Q05397 (PTK2) | Y1007 | Uniprot |
| Q07912 (TNK2) | Y859 | Uniprot |
| Q07912 (TNK2) | Y860 | Uniprot |
| Q16611 (BAK1) | Y108 | Uniprot |
| Q96T51 (RUFY1) | Y389 | Uniprot |
| Q96T51 (RUFY1) | Y400 | Uniprot |
研究背景
Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.
Phosphorylated in response to protein I/II and to LPS. Phosphorylation at Tyr-566 by SRC and by autocatalysis leads to activation and is required for STAT3 phosphorylation by BMX.
Cytoplasm.
Note: Localizes to the edges of spreading cells when complexed with BCAR1.
Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines.
Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B.
SH2 domain mediates interaction with RUFY1.
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
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