PINK1 Antibody - #AF4791
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| 製品: | PINK1 Antibody |
| カタログ: | AF4791 |
| タンパク質の説明: | Rabbit polyclonal antibody to PINK1 |
| アプリケーション: | WB |
| 反応性: | Human, Mouse |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 50-70kDa; 63kD(Calculated). |
| ユニプロット: | Q9BXM7 |
| RRID: | AB_2844780 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF4791, RRID:AB_2844780.
折りたたみ／展開
BRPK; FLJ27236; mitochondrial; PARK 6; PARK6; Phosphatase and Tensin Homolog; PINK 1; PINK1; PINK1_HUMAN; Protein kinase BRPK; PTEN induced putative kinase 1; PTEN induced putative kinase protein 1; PTEN-induced putative kinase protein 1; Serine/threonine kinase PINK1 mitochondrial; Serine/threonine protein kinase PINK1 mitochondrial; Serine/threonine-protein kinase PINK1;
免疫原
Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
- Q9BXM7 PINK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVRQALGRGLQLGRALLLRFTGKPGRAYGLGRPGPAAGCVRGERPGWAAGPGAEPRRVGLGLPNRLRFFRQSVAGLAARLQRQFVVRAWGCAGPCGRAVFLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGPDPLDTRRLQGFRLEEYLIGQSIGKGCSAAVYEATMPTLPQNLEVTKSTGLLPGRGPGTSAPGEGQERAPGAPAFPLAIKMMWNISAGSSSEAILNTMSQELVPASRVALAGEYGAVTYRKSKRGPKQLAPHPNIIRVLRAFTSSVPLLPGALVDYPDVLPSRLHPEGLGHGRTLFLVMKNYPCTLRQYLCVNTPSPRLAAMMLLQLLEGVDHLVQQGIAHRDLKSDNILVELDPDGCPWLVIADFGCCLADESIGLQLPFSSWYVDRGGNGCLMAPEVSTARPGPRAVIDYSKADAWAVGAIAYEIFGLVNPFYGQGKAHLESRSYQEAQLPALPESVPPDVRQLVRALLQREASKRPSARVAANVLHLSLWGEHILALKNLKLDKMVGWLLQQSAATLLANRLTEKCCVETKMKMLFLANLECETLCQAALLLCSWRAAL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9BXM7 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K137 | Ubiquitination | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| S228 | Phosphorylation | Q9BXM7 (PINK1) | Uniprot |
| S229 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| T257 | Phosphorylation | Q9BXM7 (PINK1) | Uniprot |
| K262 | Acetylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | Q7KZI7 (MARK2) | Uniprot |
| K319 | Ubiquitination | Uniprot | |
| S402 | Phosphorylation | Q9BXM7 (PINK1) | Uniprot |
| K433 | Ubiquitination | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S495 | Phosphorylation | Uniprot | |
| K547 | Ubiquitination | Uniprot |
PTMs - Q9BXM7 酵素として
研究背景
Protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PRKN. Targets PRKN to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PRKN in 2 steps: (1) by mediating phosphorylation at 'Ser-65' of PRKN and (2) mediating phosphorylation of ubiquitin, converting PRKN to its fully-active form. Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity).
Autophosphorylation at Ser-228 and Ser-402 is essential for Parkin/PRKN recruitment to depolarized mitochondria.
Two shorter forms of 55 kDa and 48 kDa seem to be produced by proteolytic cleavage and localize mainly in cytosol. Processed into a 52 kDa mature form by PARL or AFG3L2 following the cleavage by mitochondrial-processing peptidase (MPP) during mitochondrial import.
Mitochondrion outer membrane>Single-pass membrane protein. Mitochondrion inner membrane>Single-pass membrane protein. Cytoplasm>Cytosol.
Note: Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol.
Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development.
Interacts with PRKN. Interacts with FBXO7. Forms a complex with PRKN and PARK7. Interacts with NENF.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
研究領域
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
参考文献
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