Phospho-LIMK2 (Ser283) Antibody - #AF3346
(1)
(18)
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# AF3346, RRID:AB_2834761.
折りたたみ／展開
LIM containing protein kinase 2b; LIM domain kinase 2; LIM domain kinase 2b; LIM kinase 2b; LIM motif containing protein kinase 2b; LIMK 2b; LIMK-2; Limk2; LIMK2 b; LIMK2_HUMAN; LIMK2b;
免疫原
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
- P53671 LIMK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P53671 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K57 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| T151 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| T179 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| T278 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | O14965 (AURKA) , Q05655 (PRKCD) | Uniprot |
| S287 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K355 | Acetylation | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| T494 | Phosphorylation | O14965 (AURKA) | Uniprot |
| T505 | Phosphorylation | Q5VT25 (CDC42BPA) , O14965 (AURKA) , Q13464 (ROCK1) , O75116 (ROCK2) | Uniprot |
研究背景
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Phosphorylated on serine and/or threonine residues by ROCK1.
Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Spindle.
Cytoplasm. Nucleus.
Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus.
Cytoplasm. Nucleus.
Note: Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
Binds ROCK1 and MARF1 (Ref.9,. Interacts with NISCH (By similarity).
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
研究領域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
参考文献
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