MFF Antibody - #DF12006

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製品: MFF Antibody
カタログ: DF12006
タンパク質の説明: Rabbit polyclonal antibody to MFF
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
分子量: 26-29, 35-38 kDa; 38kD(Calculated).
ユニプロット: Q9GZY8
RRID: AB_2844811

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(92%)
クローナリティ:
Polyclonal
特異性:
MFF Antibody detects endogenous levels of total MFF.
RRID:
AB_2844811
引用形式: Affinity Biosciences Cat# DF12006, RRID:AB_2844811.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C2orf33; Chromosome 2 open reading frame 33; DKFZp666J168; GL004; Mff; MFF_HUMAN; MGC110913; Mitochondrial fission factor; OTTHUMP00000164235;

免疫原

免疫原:
Uniprot:

Q9GZY8(MFF_HUMAN) >>Visit HPA database.

遺伝子(ID):
MFF(56947)
発現特異性:
Q9GZY8 MFF_HUMAN:

Highly expressed in heart, kidney, liver, brain, muscle, and stomach.

タンパク質配列:
MSKGTSSDTSLGRVSRAAFPSPTAAEMAEISRIQYEMEYTEGISQRMRVPEKLKVAPPNADLEQGFQEGVPNASVIMQVPERIVVAGNNEDVSFSRPADLDLIQSTPFKPLALKTPPRVLTLSERPLDFLDLERPPTTPQNEEIRAVGRLKRERSMSENAVRQNGQLVRNDSLWHRSDSAPRNKISRFQAPISAPEYTVTPSPQQARVCPPHMLPEDGANLSSARGILSLIQSSTRRAYQQILDVLDENRRPVLRGGSAAATSNPHHDNVRYGISNIDTTIEGTSDDLTVVDAASLRRQIIKLNRRLQLLEEENKERAKREMVMYSITVAFWLLNSWLWFRR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
92
Xenopus
50
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9GZY8 基板として

Site PTM Type Enzyme
S21 Phosphorylation
Y35 Phosphorylation
Y39 Phosphorylation
T40 Phosphorylation
K54 Ubiquitination
S74 Phosphorylation
S93 Phosphorylation
T106 Phosphorylation
K109 Ubiquitination
K114 Ubiquitination
T115 Phosphorylation
T121 Phosphorylation
S123 Phosphorylation
T137 Phosphorylation
T138 Phosphorylation
S155 Phosphorylation Q13131 (PRKAA1)
S157 Phosphorylation
S172 Phosphorylation Q13131 (PRKAA1)
S177 Phosphorylation
S179 Phosphorylation
T200 Phosphorylation
S202 Phosphorylation
S222 Phosphorylation
S223 Phosphorylation
S229 Phosphorylation
S233 Phosphorylation
S258 Phosphorylation
S263 Phosphorylation
K302 Ubiquitination

研究背景

機能:

Plays a role in mitochondrial and peroxisomal fission. Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles.

細胞の位置付け:

Mitochondrion outer membrane>Single-pass type IV membrane protein. Peroxisome. Cytoplasmic vesicle>Secretory vesicle>Synaptic vesicle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in heart, kidney, liver, brain, muscle, and stomach.

サブユニット構造:

Homodimer. Interacts with DNM1L (By similarity).

タンパク質ファミリー:

Belongs to the Tango11 family.

参考文献

1). Valproic acid regulates MIEF1 through MST2-HIPPO to suppress breast cancer growth. Life sciences, 2022 (PubMed: 36126724) [IF=6.1]
2). Paeoniflorin Ameliorates Skeletal Muscle Atrophy in Chronic Kidney Disease via AMPK/SIRT1/PGC-1α-Mediated Oxidative Stress and Mitochondrial Dysfunction. Frontiers in Pharmacology, 2022 (PubMed: 35370668) [IF=5.6]

Application: WB    Species: rat    Sample: C2C12 cells

FIGURE 8 | PGC-1α might be important in mediating the effects of PF on TNF-α-treated C2C12 cells.(I) Representative western blots using antibodies against p-DRP1 (Ser616), DRP1, FIS1, MFF, MTFP1 and GAPDH.
3). Elucidation of SIRT-1/PGC-1α-associated mitochondrial dysfunction and autophagy in nonalcoholic fatty liver disease. Lipids in Health and Disease, 2021 (PubMed: 33902605) [IF=4.5]

Application: WB    Species: Human    Sample: HepG2 cells

Fig. 5 Effect of intervene in SIRT-1 and combine with OA on p62, Beclin-1, LC3B, SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression in HepG2 cells. HepG2 cells were treated with 1.5 mM OA for 48 h, following T6 (2 μM) or CAY (20 μM) 2 h. a The p62, Beclin-1 and LC3B protein expression was detected by Western blotting. b The same method was used to detect the SIRT-1, PGC-1a, MFN1, MFF and COX-IV protein expression. The bands were normalized using GAPDH. The images were quantified with ImageJ. Data are presented as the mean ± SD; *P < 0.05, **P < 0.01 compared with CON group; #P < 0.05, ##P < 0.01 compared with OA group

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