PFKFB3 Antibody - #DF12016

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製品: PFKFB3 Antibody
カタログ: DF12016
タンパク質の説明: Rabbit polyclonal antibody to PFKFB3
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
分子量: 58 kDa; 60kD(Calculated).
ユニプロット: Q16875
RRID: AB_2844821

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
PFKFB3 Antibody detects endogenous levels of total PFKFB3.
RRID:
AB_2844821
引用形式: Affinity Biosciences Cat# DF12016, RRID:AB_2844821.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase; 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3; 6-bisphosphatase; 6-P2ase 3; 6-P2ASE brain/placenta-type isozyme; 6PF 2 K/Fru 2,6 P2ASE brain/placenta type isozyme; 6PF 2-K/Fru 2,6 P2ase 3; 6PF-2-K/Fru-2; F263_HUMAN; fructose 6 phosphate,2 kinase/fructose 2, 6 bisphosphatase; Fructose-2; Inducible 6 phosphofructo 2 kinase/fructose 2,6 bisphosphatase; iPFK 2; iPFK-2; IPFK2; PFK/FBPase 3; PFK2; PFKFB3; Renal carcinoma antigen NY REN 56; Renal carcinoma antigen NY-REN-56; uPFK 2;

免疫原

免疫原:
Uniprot:

Q16875(F263_HUMAN) >>Visit HPA database.

遺伝子(ID):
PFKFB3(5209)
発現特異性:
Q16875 F263_HUMAN:

Ubiquitous.

タンパク質配列:
MPLELTQSRVQKIWVPVDHRPSLPRSCGPKLTNSPTVIVMVGLPARGKTYISKKLTRYLNWIGVPTKVFNVGEYRREAVKQYSSYNFFRPDNEEAMKVRKQCALAALRDVKSYLAKEGGQIAVFDATNTTRERRHMILHFAKENDFKAFFIESVCDDPTVVASNIMEVKISSPDYKDCNSAEAMDDFMKRISCYEASYQPLDPDKCDRDLSLIKVIDVGRRFLVNRVQDHIQSRIVYYLMNIHVQPRTIYLCRHGENEHNLQGRIGGDSGLSSRGKKFASALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQWKALNEIDAGVCEELTYEEIRDTYPEEYALREQDKYYYRYPTGESYQDLVQRLEPVIMELERQENVLVICHQAVLRCLLAYFLDKSAEEMPYLKCPLHTVLKLTPVAYGCRVESIYLNVESVCTHRERSEDAKKGPNPLMRRNSVTPLASPEPTKKPRINSFEEHVASTSAALPSCLPPEVPTQLPGQNMKGSRSSADSSRKH

PTMs - Q16875 基板として

Site PTM Type Enzyme
K12 Ubiquitination
S22 Phosphorylation
K97 Ubiquitination
K100 Ubiquitination
K111 Ubiquitination
K116 Ubiquitination
S172 Phosphorylation
K176 Ubiquitination
K189 Ubiquitination
Y194 Phosphorylation
K205 Ubiquitination
K214 Ubiquitination
S269 Phosphorylation O14920 (IKBKB)
S273 Phosphorylation
K277 Ubiquitination
S283 Phosphorylation
K284 Ubiquitination
K292 Ubiquitination
K302 Ubiquitination
K319 Ubiquitination
K352 Ubiquitination
K411 Ubiquitination
S461 Phosphorylation Q15418 (RPS6KA1) , P17612 (PRKACA) , Q13131 (PRKAA1) , P17252 (PRKCA) , P49137 (MAPKAPK2) , P31749 (AKT1)
T463 Phosphorylation
S467 Phosphorylation
T471 Phosphorylation
S478 Phosphorylation

研究背景

機能:

Synthesis and degradation of fructose 2,6-bisphosphate.

PTMs:

Phosphorylation by AMPK stimulates activity.

組織特異性:

Ubiquitous.

タンパク質ファミリー:

In the C-terminal section; belongs to the phosphoglycerate mutase family.

研究領域

· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

参考文献

1). Yes associated protein 1 promotes resistance to 5-fluorouracil in gastric cancer by regulating GLUT3-dependent glycometabolism reprogramming of tumor-associated macrophages. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2021 (PubMed: 33727040) [IF=3.9]

Application: WB    Species: Human    Sample: Gastric cancer (GC) cells

Fig. 3. IL13 secreted by YAP1-overexpressed GC cells stimulates resistance to 5-FU via inducing M2 subtype macrophage glycolysis reprogramming. A-C. RT-PCR was used to detect the mRNA expression of glycolysis enzymes, fatty acid and amino acid metabolism enzymes in THP1 after co-cultured with MKN-YAP1 or MKN45-Vetor. D. Protein level change of glycolysis enzymes in THP1 after co-cultured with MKN-YAP1, MKN45-Vetor, SGC7901-siYAP1 or SGC7901-NC. E. RT-PCR revealed the mRNA expression of glycolysis enzymes and M2 TAMs markers in THP1 after co-cultured with SGC7901-siYAP1 or SGC7901-NC. F-G. Relative lactate release from cells was determined by colorimetric analysis. Relative glucose uptake cells by Flow cytometry detection. All data presented are the mean ± SD (*p < 0.05, **p < 0.01) of triplicate determination from three independent experiments.
2). PFKFB3 facilitates cell proliferation and migration in anaplastic thyroid carcinoma via the WNT/β-catenin signaling pathway. Endocrine, 2024 (PubMed: 38378893) [IF=3.7]

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