RB1CC1 Antibody - #DF12022
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12022, RRID:AB_2844827.
折りたたみ／展開
FAK family kinase-interacting protein of 200 kDa; FIP200; RB1-inducible coiled-coil protein 1; RB1CC1; RBCC1_HUMAN;
免疫原
Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.
- Q8TDY2 RBCC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKLYVFLVNTGTTLTFDTELTVQTVADLKHAIQSKYKIAIQHQVLVVNGGECMAADRRVCTYSAGTDTNPIFLFNKEMILCDRPPAIPKTTFSTENDMEIKVEESLMMPAVFHTVASRTQLALEMYEVAKKLCSFCEGLVHDEHLQHQGWAAIMANLEDCSNSYQKLLFKFESIYSNYLQSIEDIKLKLTHLGTAVSVMAKIPLLECLTRHSYRECLGRLDSLPEHEDSEKAEMKRSTELVLSPDMPRTTNESLLTSFPKSVEHVSPDTADAESGKEIRESCQSTVHQQDETTIDTKDGDLPFFNVSLLDWINVQDRPNDVESLVRKCFDSMSRLDPRIIRPFIAECRQTIAKLDNQNMKAIKGLEDRLYALDQMIASCGRLVNEQKELAQGFLANQKRAENLKDASVLPDLCLSHANQLMIMLQNHRKLLDIKQKCTTAKQELANNLHVRLKWCCFVMLHADQDGEKLQALLRLVIELLERVKIVEALSTVPQMYCLAVVEVVRRKMFIKHYREWAGALVKDGKRLYEAEKSKRESFGKLFRKSFLRNRLFRGLDSWPPSFCTQKPRKFDCELPDISLKDLQFLQSFCPSEVQPFLRVPLLCDFEPLHQHVLALHNLVKAAQSLDEMSQTITDLLSEQKASVSQTSPQSASSPRMESTAGITTTTSPRTPPPLTVQDPLCPAVCPLEELSPDSIDAHTFDFETIPHPNIEQTIHQVSLDLDSLAESPESDFMSAVNEFVIEENLSSPNPISDPQSPEMMVESLYSSVINAIDSRRMQDTNVCGKEDFGDHTSLNVQLERCRVVAQDSHFSIQTIKEDLCHFRTFVQKEQCDFSNSLKCTAVEIRNIIEKVKCSLEITLKEKHQKELLSLKNEYEGKLDGLIKETEENENKIKKLKGELVCLEEVLQNKDNEFALVKHEKEAVICLQNEKDQKLLEMENIMHSQNCEIKELKQSREIVLEDLKKLHVENDEKLQLLRAELQSLEQSHLKELEDTLQVRHIQEFEKVMTDHRVSLEELKKENQQIINQIQESHAEIIQEKEKQLQELKLKVSDLSDTRCKLEVELALKEAETDEIKILLEESRAQQKETLKSLLEQETENLRTEISKLNQKIQDNNENYQVGLAELRTLMTIEKDQCISELISRHEEESNILKAELNKVTSLHNQAFEIEKNLKEQIIELQSKLDSELSALERQKDEKITQQEEKYEAIIQNLEKDRQKLVSSQEQDREQLIQKLNCEKDEAIQTALKEFKLEREVVEKELLEKVKHLENQIAKSPAIDSTRGDSSSLVAELQEKLQEEKAKFLEQLEEQEKRKNEEMQNVRTSLIAEQQTNFNTVLTREKMRKENIINDLSDKLKSTMQQQERDKDLIESLSEDRARLLEEKKKLEEEVSKLRSSSFVPSPYVATAPELYGACAPELPGESDRSAVETADEGRVDSAMETSMMSVQENIHMLSEEKQRIMLLERTLQLKEEENKRLNQRLMSQSMSSVSSRHSEKIAIRDFQVGDLVLIILDERHDNYVLFTVSPTLYFLHSESLPALDLKPGEGASGASRRPWVLGKVMEKEYCQAKKAQNRFKVPLGTKFYRVKAVSWNKKV
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TDY2 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| K35 | Ubiquitination | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T209 | Phosphorylation | Uniprot | |
| R210 | Methylation | Uniprot | |
| S212 | Phosphorylation | Uniprot | |
| R214 | Methylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| S237 | Phosphorylation | Uniprot | |
| T238 | Phosphorylation | Uniprot | |
| S243 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K387 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| S490 | Phosphorylation | Uniprot | |
| T491 | Phosphorylation | Uniprot | |
| Y496 | Phosphorylation | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| S537 | Phosphorylation | Uniprot | |
| S624 | Phosphorylation | Uniprot | |
| S629 | Phosphorylation | Uniprot | |
| T633 | Phosphorylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S644 | Phosphorylation | Uniprot | |
| T646 | Phosphorylation | Uniprot | |
| S647 | Phosphorylation | Uniprot | |
| S650 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| S653 | Phosphorylation | Uniprot | |
| T664 | Phosphorylation | Uniprot | |
| T670 | Phosphorylation | Uniprot | |
| S793 | Phosphorylation | Uniprot | |
| K828 | Ubiquitination | Uniprot | |
| K865 | Ubiquitination | Uniprot | |
| K871 | Ubiquitination | Uniprot | |
| K877 | Ubiquitination | Uniprot | |
| K883 | Acetylation | Uniprot | |
| K891 | Acetylation | Uniprot | |
| K896 | Ubiquitination | Uniprot | |
| K917 | Ubiquitination | Uniprot | |
| S943 | Phosphorylation | Uniprot | |
| K963 | Sumoylation | Uniprot | |
| K963 | Ubiquitination | Uniprot | |
| S982 | Phosphorylation | Uniprot | |
| S986 | Phosphorylation | Uniprot | |
| K989 | Ubiquitination | Uniprot | |
| T994 | Phosphorylation | Uniprot | |
| S1051 | Phosphorylation | Uniprot | |
| K1090 | Ubiquitination | Uniprot | |
| K1110 | Ubiquitination | Uniprot | |
| S1160 | Phosphorylation | Uniprot | |
| K1204 | Ubiquitination | Uniprot | |
| Y1205 | Phosphorylation | Uniprot | |
| S1222 | Phosphorylation | Uniprot | |
| K1233 | Ubiquitination | Uniprot | |
| K1247 | Ubiquitination | Uniprot | |
| K1263 | Acetylation | Uniprot | |
| S1274 | Phosphorylation | Uniprot | |
| S1323 | Phosphorylation | Uniprot | |
| S1370 | Phosphorylation | Uniprot | |
| T1440 | Phosphorylation | Uniprot | |
| S1441 | Phosphorylation | Uniprot | |
| S1482 | Phosphorylation | Uniprot | |
| S1484 | Phosphorylation | Uniprot | |
| K1581 | Ubiquitination | Uniprot |
研究背景
Involved in autophagy. Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1. Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways. Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity). Functions as a DNA-binding transcription factor. Is a potent regulator of the RB1 pathway through induction of RB1 expression. Plays a crucial role in muscular differentiation. Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity).
Nucleus. Cytoplasm. Cytoplasm>Cytosol. Preautophagosomal structure. Lysosome.
Note: Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome.
Expression levels correlated closely with those of RB1 in cancer cell lines as well as in various normal human tissues. Abundantly expressed in human musculoskeletal and cultured osteosarcoma cells.
Part of a complex consisting of ATG13/KIAA0652, ULK1 and RB1CC1. This complex associates with ATG101. Interacts with PTK2/FAK1 and PTK2B/PYK2. Interacts with GABARAP and GABARAPL1. Interacts with ATG16L1; the interaction is required for ULK1 complex-dependent autophagy. Interacts with RNF111, SKI and SMAD7 (By similarity). Interacts with COP1 in the cytoplasm of proliferating cells in response to UV stimulation. Interacts with TP53. Interacts with C9orf72. Interacts with WDR45B.
Belongs to the ATG17 family.
研究領域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
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