ECHS1 Antibody - #DF12025
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12025, RRID:AB_2844830.
折りたたみ／展開
C80529; EC 4.2.1.17; ECHS 1; Enoyl CoA hydratase 1; Enoyl CoA hydratase mitochondrial; Enoyl CoA hydratase short chain 1 mitochondrial; Enoyl Coenzyme A hydratase short chain 1; Enoyl Coenzyme A hydratase short chain 1 mitochondrial; Mitochondrial short chain enoyl coenzyme A hydratase 1; OTTHUMP00000020811; SCEH; Short chain enoyl CoA hydratase;
免疫原
- P30084 ECHM_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKTFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P30084 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y35 | Phosphorylation | Uniprot | |
| K40 | Acetylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| C62 | S-Nitrosylation | Uniprot | |
| K92 | Acetylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| R197 | Methylation | Uniprot | |
| S199 | Phosphorylation | Uniprot | |
| K204 | Acetylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| C213 | S-Nitrosylation | Uniprot | |
| C225 | S-Nitrosylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| K261 | Ubiquitination | Uniprot |
研究背景
Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.
Mitochondrion matrix.
Liver, fibroblast, muscle. Barely detectable in spleen and kidney.
Homohexamer; dimer of trimers.
Belongs to the enoyl-CoA hydratase/isomerase family.
研究領域
· Metabolism > Lipid metabolism > Fatty acid elongation.
· Metabolism > Lipid metabolism > Fatty acid degradation.
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Amino acid metabolism > Lysine degradation.
· Metabolism > Amino acid metabolism > Tryptophan metabolism.
· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Carbohydrate metabolism > Butanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Fatty acid metabolism.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.