NDE1 Antibody - #DF12040
| 製品: | NDE1 Antibody |
| カタログ: | DF12040 |
| タンパク質の説明: | Rabbit polyclonal antibody to NDE1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 40 kDa; 38kD(Calculated). |
| ユニプロット: | Q9NXR1 |
| RRID: | AB_2844845 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12040, RRID:AB_2844845.
折りたたみ／展開
FLJ20101; HOM TES 87; LIS1 interacting protein NUDE1 rat homolog; LIS1 interacting protein NUDE1; LIS4; NDE 1; NDE1; NDE1_HUMAN; Nuclear distribution gene E homolog 1; Nuclear distribution protein nudE homolog 1; NUDE 1; NudE; NudE nuclear distribution gene E homolog 1 (A. nidulans); NudE nuclear distribution gene E homolog 1; NUDE1;
免疫原
Expressed in the neuroepithelium throughout the developing brain, including the cerebral cortex and cerebellum.
- Q9NXR1 NDE1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELREFQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSEGYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDFEQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEKPRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDDSTGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGGPASGRSSKNRDGGERRPSSTSVPLGDKGLDTSCRWLSKSTTRSSSSC
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NXR1 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T7 | Phosphorylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| T131 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S154 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| T191 | Phosphorylation | Q00535 (CDK5) | Uniprot |
| S211 | Phosphorylation | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| T215 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| S223 | Phosphorylation | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| T228 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S231 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | P28482 (MAPK1) | Uniprot |
| T240 | Phosphorylation | Uniprot | |
| T243 | Phosphorylation | Uniprot | |
| T246 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K270 | Ubiquitination | Uniprot | |
| Y279 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| T286 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S307 | Phosphorylation | Uniprot | |
| T308 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot |
研究背景
Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition (By similarity).
Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Spindle. Cleavage furrow.
Note: Localizes to the interphase and S phase centrosome. During mitosis, partially associated with the mitotic spindle. Concentrates at the plus ends of microtubules coincident with kinetochores in metaphase and anaphase in a CENPF-dependent manner. Also localizes to the cleavage furrow during cytokinesis. manner. Also localizes to the cleavage furrow during cytokinesis.
Expressed in the neuroepithelium throughout the developing brain, including the cerebral cortex and cerebellum.
Self-associates. Interacts with CNTRL, LIS1, dynein, SLMAP and TCP1 (By similarity). Interacts with CENPF, dynactin, tubulin gamma, PAFAH1B1, PCM1 and PCNT. Interacts with ZNF365.
Belongs to the nudE family.
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