Transgelin 2 Antibody - #DF12053

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製品: Transgelin 2 Antibody
カタログ: DF12053
タンパク質の説明: Rabbit polyclonal antibody to Transgelin 2
アプリケーション: WB IHC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 22 kDa; 22kD(Calculated).
ユニプロット: P37802
RRID: AB_2844858

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
Transgelin 2 Antibody detects endogenous levels of total Transgelin 2.
RRID:
AB_2844858
引用形式: Affinity Biosciences Cat# DF12053, RRID:AB_2844858.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CDABP0035; epididymis tissue protein Li 7e; HA1756; KIAA0120; SM22-alpha homolog; TAGL2_HUMAN; Tagln2; Transgelin-2;

免疫原

免疫原:

A synthesized peptide derived from human Transgelin 2, corresponding to a region within the internal amino acids.

Uniprot:

P37802(TAGL2_HUMAN) >>Visit HPA database.

遺伝子(ID):
TAGLN2(8407)
発現特異性:
P37802 TAGL2_HUMAN:

Expressed in epididymis (at protein level).

タンパク質配列:
MANRGPAYGLSREVQQKIEKQYDADLEQILIQWITTQCRKDVGRPQPGRENFQNWLKDGTVLCELINALYPEGQAPVKKIQASTMAFKQMEQISQFLQAAERYGINTTDIFQTVDLWEGKNMACVQRTLMNLGGLAVARDDGLFSGDPNWFPKKSKENPRNFSDNQLQEGKNVIGLQMGTNRGASQAGMTGYGMPRQIL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
82
Xenopus
75
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

組織特異性:

Expressed in epididymis (at protein level).

タンパク質ファミリー:

Belongs to the calponin family.

参考文献

1). TAGLN2 induces resistance signature ISGs by activating AKT-YBX1 signal with dual pathways and mediates the IFN-related DNA damage resistance in gastric cancer. Cell death & disease, 2024 (PubMed: 39168971) [IF=8.1]

Application: IHC    Species: Human    Sample: gastric cancer (GC) tissue

Fig. 1. Aberrantly upregulated expression of TAGLN2 results in the induction of ISGs. A Aberrantly upregulated TAGLN2 protein expression was assessed by IHC analysis in gastric cancer (GC) tissue microarray (TMA) chips containing 75 tumor tissues and their paired normal counterparts. B Cellular TAGLN2 expression in tumor tissues vs. paired normal counterparts was evaluated in the membrane, cytoplasm and nucleus. Distant metastases (M), T stage and overall cancer stage (Stage I~IV) were significantly correlated with TAGLN2 expression in the cytoplasm. C Representative IHC images show that abnormal TAGLN2 expression level increases as GC progresses (Stage I~IV). The results were obtained by multiplying the PP by the IS (immunoreactive score = PP × IS). *P 
2). Comparative proteomics identify HSP90A, STIP1 and TAGLN‑2 in serum extracellular vesicles as potential circulating biomarkers for human adenomyosis. Experimental and Therapeutic Medicine, 2022 (PubMed: 35495589) [IF=2.4]

Application: WB    Species: human    Sample: T‑AMEVs and B‑AMEVs

Figure 4. | HSP90A, STIP1 and TAGLN‑2 are expressed in B‑AMEVs and T‑AMEVs. (D and E) HSP90A, STIP1 and TAGLN‑2 were present in T‑AMEVs and B‑AMEVs from patients with adenomyosis,but not in those of patients in the control group.
3). Overexpression of TAGLN2 regulated by non-coding RNAs refering to poor prognosis and tumor immune cell infiltration in hepatocellular carcinoma. Research Square, 2023

Application: WB    Species: Human    Sample: HCC tissues

Figure 10. Validation results of TAGLN2 expression in HCC. (a) qRT-PCR analysis of TAGLN2 mRNA expression in 20 pairs of HCC tissues and adjacent non-tumor tissues. (b) Western blot detection of TAGLN2 protein expression and relative quantification in the mean values of three HCC tumor tissues and three neighboring tissues showed elevated TAGLN2 protein expression in HCC tumor tissues. (c) IHC analysis of TAGLN2 stained significantly darker in tumor tissues and had higher protein expression levels than adjacent tissues. (*P < 0.05, **P< 0.01, ***P < 0.001).

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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