SQLE Antibody - #DF12063
| 製品: | SQLE Antibody |
| カタログ: | DF12063 |
| タンパク質の説明: | Rabbit polyclonal antibody to SQLE |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 64 kDa; 64kD(Calculated). |
| ユニプロット: | Q14534 |
| RRID: | AB_2844868 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12063, RRID:AB_2844868.
折りたたみ／展開
ERG1; ERG1_HUMAN; SE; SQLE; Squalene epoxidase; Squalene monooxygenase;
免疫原
- Q14534 ERG1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWTFLGIATFTYFYKKFGDFITLANREVLLCVLVFLSLGLVLSYRCRHRNGGLLGRQQSGSQFALFSDILSGLPFIGFFWAKSPPESENKEQLEARRRRKGTNISETSLIGTAACTSTSSQNDPEVIIVGAGVLGSALAAVLSRDGRKVTVIERDLKEPDRIVGEFLQPGGYHVLKDLGLGDTVEGLDAQVVNGYMIHDQESKSEVQIPYPLSENNQVQSGRAFHHGRFIMSLRKAAMAEPNAKFIEGVVLQLLEEDDVVMGVQYKDKETGDIKELHAPLTVVADGLFSKFRKSLVSNKVSVSSHFVGFLMKNAPQFKANHAELILANPSPVLIYQISSSETRVLVDIRGEMPRNLREYMVEKIYPQIPDHLKEPFLEATDNSHLRSMPASFLPPSSVKKRGVLLLGDAYNMRHPLTGGGMTVAFKDIKLWRKLLKGIPDLYDDAAIFEAKKSFYWARKTSHSFVVNILAQALYELFSATDDSLHQLRKACFLYFKLGGECVAGPVGLLSVLSPNPLVLIGHFFAVAIYAVYFCFKSEPWITKPRALLSSGAVLYKACSVIFPLIYSEMKYMVH
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14534 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K90 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K274 | Ubiquitination | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| K373 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K436 | Ubiquitination | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| S559 | Phosphorylation | Uniprot | |
| Y566 | Phosphorylation | Uniprot | |
| S567 | Phosphorylation | Uniprot |
研究背景
Catalyzes the stereospecific oxidation of squalene to (S)-2,3-epoxysqualene, and is considered to be a rate-limiting enzyme in steroid biosynthesis.
Ubiquitinated by MARCHF6 in response to high cholesterol levels in intracellular membranes, leading to proteasomal degradation.
Microsome membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Peripheral membrane protein.
Detected in liver (at protein level).
Interacts (via N-terminal domain) with MARCHF6. Interacts with SMIM22; this interaction modulates lipid droplet formation.
The C-terminal hydrophobic region contains two helices that mediate interaction with membranes. Contrary to predictions, this region does not contain transmembrane helices.
The N-terminal region mediates interaction with MARCHF6, leading to SQLE ubiquitination and proteasomal degradation when cholosterol levels are high (PubMed:24449766, PubMed:26434806, PubMed:28972164). The first part of the N-terminal region contains a hydrophobic region that inserts into the membrane; contrary to predictions, there are no transmembrane helices (PubMed:26434806). The second part contains a region that can form an amphipathic region that associates with membranes. This region is ejected from the membrane by high cholesterol levels and becomes disordered in an aqueous environment. This is critical for cholesterol-dependent proteasomal degradation. Additional parts of the N-terminal region are predicted to be disordered and contribute to flagging the protein for proteasomal degradation already under basal conditions (PubMed:28972164).
Belongs to the squalene monooxygenase family.
研究領域
· Metabolism > Lipid metabolism > Steroid biosynthesis.
· Metabolism > Global and overview maps > Metabolic pathways.
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