HEY1 Antibody - #DF12076

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製品: HEY1 Antibody
カタログ: DF12076
タンパク質の説明: Rabbit polyclonal antibody to HEY1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 32-34 kDa; 33kD(Calculated).
ユニプロット: Q9Y5J3
RRID: AB_2844881

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(83%), Horse(100%), Sheep(83%), Rabbit(83%), Dog(83%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
HEY1 Antibody detects endogenous levels of total HEY1.
RRID:
AB_2844881
引用形式: Affinity Biosciences Cat# DF12076, RRID:AB_2844881.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Basic helix loop helix protein OAF1; bHLHb31; Cardiovascular helix loop-helix factor 2; Cardiovascular helix-loop-helix factor 2; CHF-2; CHF2; Class B basic helix-loop-helix protein 31; Hairy and enhancer of split related protein 1; Hairy and enhancer of split-related protein 1; Hairy enhancer-of-split related with YRPW motif protein 1; Hairy related transcription factor 1; Hairy-related transcription factor 1; Hairy/enhancer of split related with YRPW motif 1; Hairy/enhancer-of-split related with YRPW motif protein 1; HERP2; HES related repressor protein 1; HES-related repressor protein 1; HES-related repressor protein 2; HESR-1; HESR1; HEY 1; hey1; HEY1_HUMAN; hHRT1; HRT-1; HRT1; OAF1;

免疫原

免疫原:
Uniprot:

Q9Y5J3(HEY1_HUMAN) >>Visit HPA database.

遺伝子(ID):
HEY1(23462)
発現特異性:
Q9Y5J3 HEY1_HUMAN:

Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.

タンパク質配列:
MKRAHPEYSSSDSELDETIEVEKESADENGNLSSALGSMSPTTSSQILARKRRRGIIEKRRRDRINNSLSELRRLVPSAFEKQGSAKLEKAEILQMTVDHLKMLHTAGGKGYFDAHALAMDYRSLGFRECLAEVARYLSIIEGLDASDPLRVRLVSHLNNYASQREAASGAHAGLGHIPWGTVFGHHPHIAHPLLLPQNGHGNAGTTASPTEPHHQGRLGSAHPEAPALRAPPSGSLGPVLPVVTSASKLSPPLLSSVASLSAFPFSFGSFHLLSPNALSPSAPTQAANLGKPYRPWGTEIGAF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
83
Sheep
83
Dog
83
Chicken
83
Rabbit
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5J3 基板として

Site PTM Type Enzyme
S10 Phosphorylation
S11 Phosphorylation
S13 Phosphorylation
S25 Phosphorylation
S45 Phosphorylation
K59 Acetylation
S68 Phosphorylation
S78 Phosphorylation
T97 Phosphorylation
Y161 Phosphorylation
S163 Phosphorylation
S246 Phosphorylation

研究背景

機能:

Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3. Involved in the regulation of liver cancer cells self-renewal.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs.

サブユニット構造:

Self-associates. Interacts with HES1 and HEYL. Interacts with HDAC1, NCOR1 and SIN3A. Interacts with GATA4 and GATA6. Interacts with CCDC89/BOIP.

タンパク質ファミリー:

Belongs to the HEY family.

研究領域

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Breast cancer.   (View pathway)

参考文献

1). Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats. Stem Cells International, 2019 (PubMed: 31249601) [IF=4.3]

Application: WB    Species: rat    Sample: MSCs

Figure 3|: Expression of NPC markers and Notch pathway in NPC exosome-treated MSCs. (a) Gene expression of NPC markers (collagen II,Aggrecan, and Sox9) was significantly upregulated in exosome-treated MSCs in 7, 14, and 21 days. (b) Expression of related protein was normalized to GAPDH. (c) Expression of Notch pathway-related genes in exosome-treated MSCs was detected by qRT-PCR. (d)Western blot analysis of Notch pathway-related protein in exosome-treated MSCs. All data were showed as mean ± SD. N = 3. ∗P < 0 05, ∗∗P < 0 01, and ∗∗∗P < 0 001.
2). γ‐secretase inhibitors suppress IL‐20‐mediated osteoclastogenesis via Notch signaling and are affected by Notch2 in vitro. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2022 (PubMed: 35384009) [IF=3.7]

Application: WB    Species: Human    Sample:

FIGURE 2 20 ng/mL IL-20 treated the pre-osteoclasts for 48 h meanwhile control groups were treated with 0 ng/mL IL-20. Detected mRNA and analysed by GO Analysis and Pathway Analysis. (A) Bubble chart analysis based on KEGG databases. (B) Gene Set Enrichment Analysis of osteoclast differentiation with control groups and IL-20 treat groups. (C) Gene heatmap showed four classical gene were enriched to Notch signal pathway (Red – up regulated and green – down regulated). (D-E) The expression of Notch1 and Notch2 mRNA with different concentration of IL-20 stimulation. Analysed by one-way ANOVA with Tukey's post hoc analysis with three independent experiments. *P < .05 vs the control group; ns, not significant. (F) mRNA expression of Notch1, Notch2, Hes1 and Hey1 at 20 ng/mL IL-20 stimulation using by qRT-PCR and analysed by Student's t test. Bars represent mean ± SEM from three independent experiments. *P < .05 vs the control group; ns, not significant. (G-H) Western blotting data and analysis of Notch signal pathway. Student's t test was used to detect difference. Bars represent mean ± SEM from three independent experiments. *P < .05 vs the control group; ns, not significant

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