IST1 Antibody - #DF12077
| 製品: | IST1 Antibody |
| カタログ: | DF12077 |
| タンパク質の説明: | Rabbit polyclonal antibody to IST1 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse |
| 予測: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 40 kDa; 40kD(Calculated). |
| ユニプロット: | P53990 |
| RRID: | AB_2844882 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12077, RRID:AB_2844882.
折りたたみ／展開
hIST1; Increased sodium tolerance 1; Increased sodium tolerance 1 homolog; IST1 homolog; IST1_HUMAN; KIAA0174; MAPK activating protein PM28; MGC117220; MGC128433; Putative MAPK-activating protein PM28;
免疫原
- P53990 IST1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESVDDINADKNISSAQIVGPGPKPEASAKLPSRPADNYDNFVLPELPSVPDTLPTASAGASTSASEDIDFDDLSRRFEELKKKT
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P53990 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| K7 | Ubiquitination | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| Y43 | Phosphorylation | Uniprot | |
| K48 | Ubiquitination | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| R147 | Methylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K205 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| K249 | Sumoylation | Uniprot | |
| T276 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S294 | Phosphorylation | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| T335 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| K361 | Ubiquitination | Uniprot |
研究背景
ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.
Cytoplasmic vesicle. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Midbody. Nucleus envelope.
Note: Localizes to centrosome and midbody of dividing cells (PubMed:19129480, PubMed:19129479, PubMed:20719964). Colocalized with SPART to the ends of Flemming bodies during cytokinesis (PubMed:20719964). Localizes to the nuclear envelope during late anaphase (PubMed:26040712).
Interacts with CHMP1A, CHMP1B, VPS4A and VTA1. Interacts with SPAST, STAMBP, and USP8. May interact with VPS37B. May associate with the ESCRT-I complex. Interacts with MITD1, in competition with VSP4. Interacts with SPART (via MIT domain); leading to the recruitment of SPART to midbodies. Interacts with SPAST.
Belongs to the IST1 family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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