Piezo1 Antibody - #DF12083

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製品: Piezo1 Antibody
カタログ: DF12083
タンパク質の説明: Rabbit polyclonal antibody to Piezo1
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Dog, Chicken, Xenopus
分子量: 287 kDa; 287kD(Calculated).
ユニプロット: Q92508
RRID: AB_2844888

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(92%), Xenopus(83%)
クローナリティ:
Polyclonal
特異性:
Piezo1 Antibody detects endogenous levels of total Piezo1.
RRID:
AB_2844888
引用形式: Affinity Biosciences Cat# DF12083, RRID:AB_2844888.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

DHS; Fam38a; Family with sequence similarity 38 member A; KIAA0233; Membrane protein induced by beta-amyloid treatment; Mib; PIEZ1_HUMAN; Piezo-type mechanosensitive ion channel component 1; PIEZO1; Protein FAM38A; Protein FAM38B; Protein PIEZO1;

免疫原

免疫原:
Uniprot:

Q92508(PIEZ1_HUMAN) >>Visit HPA database.

遺伝子(ID):
PIEZO1(9780)
発現特異性:
Q92508 PIEZ1_HUMAN:

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level).

タンパク質配列:
MEPHVLGAVLYWLLLPCALLAACLLRFSGLSLVYLLFLLLLPWFPGPTRCGLQGHTGRLLRALLGLSLLFLVAHLALQICLHIVPRLDQLLGPSCSRWETLSRHIGVTRLDLKDIPNAIRLVAPDLGILVVSSVCLGICGRLARNTRQSPHPRELDDDERDVDASPTAGLQEAATLAPTRRSRLAARFRVTAHWLLVAAGRVLAVTLLALAGIAHPSALSSVYLLLFLALCTWWACHFPISTRGFSRLCVAVGCFGAGHLICLYCYQMPLAQALLPPAGIWARVLGLKDFVGPTNCSSPHALVLNTGLDWPVYASPGVLLLLCYATASLRKLRAYRPSGQRKEAAKGYEARELELAELDQWPQERESDQHVVPTAPDTEADNCIVHELTGQSSVLRRPVRPKRAEPREASPLHSLGHLIMDQSYVCALIAMMVWSITYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGMTLCCLRYVWAMDLRPELPTTLGPVSLRQLGLEHTRYPCLDLGAMLLYTLTFWLLLRQFVKEKLLKWAESPAALTEVTVADTEPTRTQTLLQSLGELVKGVYAKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYSLWRKLLKAFWWLVVAYTMLVLIAVYTFQFQDFPAYWRNLTGFTDEQLGDLGLEQFSVSELFSSILVPGFFLLACILQLHYFHRPFMQLTDMEHVSLPGTRLPRWAHRQDAVSGTPLLREEQQEHQQQQQEEEEEEEDSRDEGLGVATPHQATQVPEGAAKWGLVAERLLELAAGFSDVLSRVQVFLRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCVIIVCKMLYQLKVVNPQEYSSNCTEPFPNSTNLLPTEISQSLLYRGPVDPANWFGVRKGFPNLGYIQNHLQVLLLLVFEAIVYRRQEHYRRQHQLAPLPAQAVFASGTRQQLDQDLLGCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFLVTLHGCWLVAILTRRHRQAIARLWPNYCLFLALFLLYQYLLCLGMPPALCIDYPWRWSRAVPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQRMAGVNTDRLEPLRGEPNPVPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVTGATRISIFGLGYLLACFYLLLFGTALLQRDTRARLVLWDCLILYNVTVIISKNMLSLLACVFVEQMQTGFCWVIQLFSLVCTVKGYYDPKEMMDRDQDCLLPVEEAGIIWDSVCFFFLLLQRRVFLSHYYLHVRADLQATALLASRGFALYNAANLKSIDFHRRIEEKSLAQLKRQMERIRAKQEKHRQGRVDRSRPQDTLGPKDPGLEPGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEAVPEDPRPSAQSAFQLAYQAWVTNAQAVLRRRQQEQEQARQEQAGQLPTGGGPSQEVEPAEGPEEAAAGRSHVVQRVLSTAQFLWMLGQALVDELTRWLQEFTRHHGTMSDVLRAERYLLTQELLQGGEVHRGVLDQLYTSQAEATLPGPTEAPNAPSTVSSGLGAEEPLSSMTDDMGSPLSTGYHTRSGSEEAVTDPGEREAGASLYQGLMRTASELLLDRRLRIPELEEAELFAEGQGRALRLLRAVYQCVAAHSELLCYFIIILNHMVTASAGSLVLPVLVFLWAMLSIPRPSKRFWMTAIVFTEIAVVVKYLFQFGFFPWNSHVVLRRYENKPYFPPRILGLEKTDGYIKYDLVQLMALFFHRSQLLCYGLWDHEEDSPSKEHDKSGEEEQGAEEGPGVPAATTEDHIQVEARVGPTDGTPEPQVELRPRDTRRISLRFRRRKKEGPARKGAAAIEAEDREEEEGEEEKEAPTGREKRPSRSGGRVRAAGRRLQGFCLSLAQGTYRPLRRFFHDILHTKYRAATDVYALMFLADVVDFIIIIFGFWAFGKHSAATDITSSLSDDQVPEAFLVMLLIQFSTMVVDRALYLRKTVLGKLAFQVALVLAIHLWMFFILPAVTERMFNQNVVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSSWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLVRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIIPFTAQAYEELSRQFDPQPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKRELYNGTADITLRFTWNFQRDLAKGGTVEYANEKHMLALAPNSTARRQLASLLEGTSDQSVVIPNLFPKYIRAPNGPEANPVKQLQPNEEADYLGVRIQLRREQGAGATGFLEWWVIELQECRTDCNLLPMVIFSDKVSPPSLGFLAGYGIMGLYVSIVLVIGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTREKE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
92
Xenopus
83
Sheep
67
Zebrafish
64
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92508 基板として

Site PTM Type Enzyme
K113 Ubiquitination
S165 Phosphorylation
T167 Phosphorylation
T175 Phosphorylation
T179 Phosphorylation
K346 Ubiquitination
K540 Ubiquitination
S732 Phosphorylation
T734 Phosphorylation
S758 Phosphorylation
Y821 Phosphorylation
T822 Phosphorylation
T1016 Phosphorylation
T1027 Phosphorylation
Y1165 Phosphorylation
Y1195 Phosphorylation
K1267 Acetylation
K1334 Ubiquitination
K1345 Ubiquitination
K1351 Ubiquitination
K1360 Ubiquitination
K1381 Ubiquitination
S1391 Phosphorylation
S1395 Phosphorylation
S1396 Phosphorylation
T1479 Phosphorylation
S1509 Phosphorylation
T1510 Phosphorylation
S1619 Phosphorylation
S1621 Phosphorylation
T1626 Phosphorylation
S1636 Phosphorylation
Y1638 Phosphorylation
T1644 Phosphorylation
S1646 Phosphorylation
S1820 Phosphorylation
T1854 Phosphorylation
K1903 Ubiquitination
K1953 Ubiquitination
N2294 N-Glycosylation
K2313 Ubiquitination
K2372 Ubiquitination
K2479 Ubiquitination

研究背景

機能:

Pore-forming subunit of a mechanosensitive non-specific cation channel. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensor that promotes endothelial cell organization and alignment in the direction of blood flow through calpain activation. Plays a key role in blood vessel formation and vascular structure in both development and adult physiology (By similarity).

細胞の位置付け:

Endoplasmic reticulum membrane>Multi-pass membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Cell membrane>Multi-pass membrane protein. Cell projection>Lamellipodium membrane.
Note: In erythrocytes, located in the plasma membrane (PubMed:22529292, PubMed:23479567). Accumulates at the leading apical lamellipodia of endothelial cells in response to shear stress (PubMed:25119035).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in numerous tissues. In normal brain, expressed exclusively in neurons, not in astrocytes. In Alzheimer disease brains, expressed in about half of the activated astrocytes located around classical senile plaques. In Parkinson disease substantia nigra, not detected in melanin-containing neurons nor in activated astrocytes. Expressed in erythrocytes (at protein level).

サブユニット構造:

Homotrimer. Interacts with PKD2. Interacts with STOML3.

タンパク質ファミリー:

Belongs to the PIEZO (TC 1.A.75) family.

参考文献

1). Gastric mechanosensitive channel Piezo1 regulates ghrelin production and food intake. Nature metabolism, 2024 (PubMed: 38467889) [IF=20.8]
2). Self-amplifying loop of NF-κB and periostin initiated by PIEZO1 accelerates mechano-induced senescence of nucleus pulposus cells and intervertebral disc degeneration. Molecular Therapy, 2022 (PubMed: 35619555) [IF=12.4]

Application: WB    Species: Human    Sample: NP tissues

Figure 5. Mechano-stress initiates the positive loop of NF-kB and periostin via PIEZO1 (A) Immunoblotting results of three control and three degenerated human NP tissues. C, control; D, degenerated. (B) Quantitation of Ca2+ influx changes in hNPCs after 5 mM Yoda1 stimulation (left panel), and representative fluorescence images at indicated time points (right panel). Yoda1 was administrated at 10 s. (C) Representative images of Fluo-8 AM in hNPCs cultured on different hydrogels. Scale bar = 100 mm. (D) Representative b-Gal staining images of hNPCs treated with vehicle or Yoda1 for 24 h (left panel), and quantitation of b-Gal+ senescent cells (right panel). n = 3 biologically independent samples. Scale bar, 100 mm. (E–G) qPCR results of senescence (E), SASP (F), and matrix metabolism (G) markers in hNPCs treated with vehicle or Yoda1 for 24 h. n = 3 biologically independent samples. (H) Representative immunostaining images of NF-kB p65 (red) in hNPCs under indicated treatments for 30 min. DAPI (blue) indicates nuclei. (I) Quantification of p65 localization in (H), presented as mean ± SD. In total, 237 cells from the vehicle group and 232 cells from the Yoda1 group were analyzed. *Represents the statistical analysis was conducted in terms of the proportion of N > C between the two groups and the p < 0.05. n = 3 biologically independent samples. Scale bar = 25 mm. (J) Immunoblotting results of hNPCs treated with vehicle or Yoda1 for 48 h. (K and L) Representative b-Gal staining images of hNPCs under indicated conditions for 24 h (K), and quantitation of b-Gal+ senescent cells (L). n = 3 biologically independent samples. Scale bar, 100 mm. (M) Relative mRNA expression of the matrix metabolism and senescence markers and POSTN in hNPCs under indicated conditions. n = 3 biologically independent samples. (N and O) Representative T2WI image (N) and MRI Pfirrmann grade (O) of rat tail IVDs with indicated administration. n = 9 discs.
3). Zuogui pill disrupt the malignant cycle in breast cancer bone metastasis through the Piezo1-Notch-1-GPX4 pathway and active molecules fishing. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38103318) [IF=7.9]
4). Gsmtx4 Alleviated Osteoarthritis through Piezo1/Calcineurin/NFAT1 Signaling Axis under Excessive Mechanical Strain. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023 (PubMed: 36835440) [IF=5.6]

Application: IF/ICC    Species: human and rat    Sample:

Figure 1. Piezo1 was up-regulated in the articular cartilage of OA patients and rats. (a,c) Representative images of HE, Safranin-O/Fast Green staining of intact and damaged areas in OA patients’ articular, Sham, and OA rat cartilage, and OARSI scores (n = 5). (b,d) Immunohistochemistry staining of intact and damaged areas in OA patients’ articular, Sham, and OA rat cartilage, and the percentage of Piezo1 positive cells (n = 5). (e) Immunofluorescence staining and quantified results of Piezo1 in chondrocytes exposed to mechanical strain for 0 h, 6 h, 12 h, 24 h, and 48 h (n = 3). (f,g) RT-qPCR and Western blot analysis of Piezo1 in chondrocytes exposed to mechanical strain for 0 h, 6 h, 12 h, 24 h, and 48 h. * p < 0.05; ** p < 0.01; ns, no significant differences. Mann–Whitney U test for (a,c), Student’s t-test for (b,d), one-way ANOVA with Bonferroni’s test for (e–g); scale bar: 100 μm.

Application: WB    Species: human and rat    Sample:

Figure 1. Piezo1 was up-regulated in the articular cartilage of OA patients and rats. (a,c) Representative images of HE, Safranin-O/Fast Green staining of intact and damaged areas in OA patients’ articular, Sham, and OA rat cartilage, and OARSI scores (n = 5). (b,d) Immunohistochemistry staining of intact and damaged areas in OA patients’ articular, Sham, and OA rat cartilage, and the percentage of Piezo1 positive cells (n = 5). (e) Immunofluorescence staining and quantified results of Piezo1 in chondrocytes exposed to mechanical strain for 0 h, 6 h, 12 h, 24 h, and 48 h (n = 3). (f,g) RT-qPCR and Western blot analysis of Piezo1 in chondrocytes exposed to mechanical strain for 0 h, 6 h, 12 h, 24 h, and 48 h. * p < 0.05; ** p < 0.01; ns, no significant differences. Mann–Whitney U test for (a,c), Student’s t-test for (b,d), one-way ANOVA with Bonferroni’s test for (e–g); scale bar: 100 μm.
5). Effect of whole body vibration therapy in the rat model of steroid-induced osteonecrosis of the femoral head. Frontiers in cell and developmental biology, 2023 (PubMed: 37876552) [IF=5.5]

Application: IHC    Species: Rat    Sample:

FIGURE 4 In (A), the immunohistochemical results of Piezo1, RUNX2, BMP2, CD31, HIF-a, and VEGF are presented. Additionally, (B–G) display the corresponding quantitative immunohistochemical results. In comparison to the model group, both the blank and WBVT groups exhibited significantly higher expression levels of Piezo1, RUNX2, BMP2, CD31, HIF-a, and VEGF. These differences were statistically significant (p < 0.05).
6). Fibrotic matrix induces mesenchymal transformation of epithelial cells in oral submucous fibrosis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2023 (PubMed: 34528373) [IF=5.3]
7). Trimethylamine-N-oxide sensitizes chondrocytes to mechanical loading through the upregulation of Piezo1. FOOD AND CHEMICAL TOXICOLOGY, 2023 (PubMed: 36925039) [IF=4.3]
8). Mechanical and signaling responses of unloaded rat soleus muscle to chronically elevated β-myosin activity. Archives of biochemistry and biophysics, 2024 (PubMed: 38492659) [IF=3.9]
9). Mechanical stretching induces fibroblasts apoptosis through activating Piezo1 and then destroying actin cytoskeleton. International Journal of Medical Sciences, 2023 (PubMed: 37213676) [IF=3.6]

Application: IHC    Species: Human    Sample: anterior vaginal wall tissue

Figure 3 Detection of Piezo1 expression and apoptosis rate of the anterior vaginal wall tissue. A, Piezo1 immunohistochemistry staining of the anterior vaginal wall tissue, magnification: ×200; B, semiquantitative assay of Piezo1 expression using Image J; C, TUNEL staining of the anterior vaginal wall tissue, magnification: 200×; D, The ratio of TUNEL-positive cells to the total number of cells. *** indicates p < 0.001. (CON: the anterior vaginal wall tissue obtained from patients without SUI; SUI: the anterior vaginal wall tissue obtained from patients with SUI).

Application: WB    Species: Human    Sample: anterior vaginal wall tissue

Figure 4 The effects of mechanical stretching on apoptosis rate and actin cytoskeleton disassembly in hAVWFs. A, Piezo1 expression in hAVWFs was detected by Western blotting and normalized into GAPDH; B, Quantity One was used to quantify the energy band strength; C, Piezo1 mRNA levels in hAVWFs was quantified by real-time RT-PCR and normalized to GAPDH; D, hAVWFs were stained with phalloidin and imaged by fluorescence microscopy. Red fluorescence delineates the cell cytoplasm; blue fluorescence delineates nuclei (magnification: 200×); E, The relative cell surface areas were quantified by ImageJ software; F, Cell apoptosis was assessed by flow cytometry analysis; G, Quantified apoptosis rates in each group. *** indicates p < 0.001; white arrows show the direction of mechanical stretching. (CON: hAVWFs isolated from patients without SUI; SUI: hAVWFs isolated from patients with SUI; MS: hAVWFs isolated from patients without SUI that were exposed to mechanical stretching).
10). Low-Intensity Pulsed Ultrasound Attenuates Postoperative Neurocognitive Impairment and Salvages Hippocampal Synaptogenesis in Aged Mice. Brain Sciences, 2023 (PubMed: 37190622) [IF=3.3]

Application: WB    Species: Mouse    Sample:

Figure 5. LIPUS inhibits Piezo1 expression after anesthesia/surgery in aged mice. (A) The mRNA expression of Piezo1 was qualified by RT-qPCR among the four groups (n = 6 per group). (B) Western blotting detection of Piezo1 among the four groups (n = 4 per group). (C) Immunofluorescence detection of Piezo1 expression in CA1 (C,D), CA3 (C,E), and DG (C,F) regions among the four groups (n = 3 per group). Data were presented as Mean ± SEM. One-way ANOVA followed by Bonferroni’s multiple comparisons tests.
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