製品: NSUN2 Antibody
カタログ: DF12103
タンパク質の説明: Rabbit polyclonal antibody to NSUN2
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 90-100 kDa; 86kD(Calculated).
ユニプロット: Q08J23
RRID: AB_2844908

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(92%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
NSUN2 Antibody detects endogenous levels of total NSUN2.
RRID:
AB_2844908
引用形式: Affinity Biosciences Cat# DF12103, RRID:AB_2844908.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

5 methycytoisine methyltransferase; D13Wsu123e; FLJ20303; hTrm4; MISU; Myc induced SUN domain containing protein; NOL1/NOP2/Sun domain family 2; NOL1/NOP2/Sun domain family 2 protein; NOL1/NOP2/Sun domain family member 2; NSUN 2; NSUN2; NSUN2_HUMAN; SAKI; Substrate of AIM1/Aurora kinase B; TRM4; tRNA (cytosine 5 ) methyltransferase; tRNA (cytosine 5 ) methyltransferase NSUN2; tRNA (cytosine(34)-C(5))-methyltransferase; tRNA (cytosine-5-)-methyltransferase; tRNA methyltransferase 4 homolo; tRNA methyltransferase 4 homolog;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q08J23 NSUN2_HUMAN:

Expressed in adult and fetal brain and in lymphoblastoid cells.

タンパク質配列:
MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Chicken
83
Xenopus
75
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08J23 基板として

Site PTM Type Enzyme
K28 Acetylation
K28 Sumoylation
K28 Ubiquitination
K43 Sumoylation
K43 Ubiquitination
K46 Sumoylation
K46 Ubiquitination
Y51 Phosphorylation
Y52 Phosphorylation
T77 Phosphorylation
T81 Phosphorylation
Y83 Phosphorylation
K84 Ubiquitination
S85 Phosphorylation
K88 Sumoylation
K88 Ubiquitination
C93 S-Nitrosylation
K95 Ubiquitination
K97 Sumoylation
K100 Sumoylation
K100 Ubiquitination
K111 Sumoylation
K111 Ubiquitination
T129 Phosphorylation
K138 Ubiquitination
S139 Phosphorylation Q96GD4 (AURKB)
K144 Ubiquitination
S151 Phosphorylation
T153 Phosphorylation
S165 Phosphorylation
K179 Ubiquitination
S189 Phosphorylation
K229 Ubiquitination
S233 Phosphorylation
S244 Phosphorylation
S245 Phosphorylation
R256 Methylation
K257 Ubiquitination
K279 Sumoylation
K279 Ubiquitination
K285 Ubiquitination
K286 Sumoylation
K286 Ubiquitination
S339 Phosphorylation
S349 Phosphorylation
K356 Ubiquitination
K365 Ubiquitination
K369 Sumoylation
K369 Ubiquitination
K396 Ubiquitination
K437 Sumoylation
K437 Ubiquitination
K441 Ubiquitination
K445 Sumoylation
K445 Ubiquitination
T449 Phosphorylation
S452 Phosphorylation
T453 Phosphorylation
S456 Phosphorylation
T461 Phosphorylation
K464 Acetylation
K464 Sumoylation
K464 Ubiquitination
T466 Phosphorylation
S473 Phosphorylation
S475 Phosphorylation
T477 Phosphorylation
K497 Ubiquitination
K508 Acetylation
K511 Sumoylation
K511 Ubiquitination
K516 Sumoylation
K516 Ubiquitination
K535 Ubiquitination
K557 Sumoylation
Y561 Phosphorylation
S564 Phosphorylation
K565 Ubiquitination
R568 Methylation
K577 Sumoylation
K577 Ubiquitination
K579 Sumoylation
K579 Ubiquitination
T583 Phosphorylation
K586 Acetylation
K586 Ubiquitination
S593 Phosphorylation
Y609 Phosphorylation
S617 Phosphorylation
T621 Phosphorylation
S623 Phosphorylation
K628 Ubiquitination
K640 Sumoylation
K640 Ubiquitination
Y646 Phosphorylation
K650 Sumoylation
K650 Ubiquitination
K654 Sumoylation
K654 Ubiquitination
K660 Sumoylation
K660 Ubiquitination
K692 Sumoylation
K692 Ubiquitination
K712 Ubiquitination
S743 Phosphorylation
S751 Phosphorylation
T755 Phosphorylation
C758 S-Nitrosylation

研究背景

機能:

RNA cytosine C(5)-methyltransferase that methylates cytosine to 5-methylcytosine (m5C) in various RNAs, such as tRNAs, mRNAs and some long non-coding RNAs (lncRNAs). Involved in various processes, such as epidermal stem cell differentiation, testis differentiation and maternal to zygotic transition during early development: acts by increasing protein synthesis; cytosine C(5)-methylation promoting tRNA stability and preventing mRNA decay. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors. tRNA methylation is required generation of RNA fragments derived from tRNAs (tRFs). Also mediates C(5)-methylation of mitochondrial tRNAs. Catalyzes cytosine C(5)-methylation of mRNAs, leading to stabilize them and prevent mRNA decay: mRNA stabilization involves YBX1 that specifically recognizes and binds m5C-modified transcripts. Cytosine C(5)-methylation of mRNAs also regulates mRNA export: methylated transcripts are specifically recognized by THOC4/ALYREF, which mediates mRNA nucleo-cytoplasmic shuttling. Also mediates cytosine C(5)-methylation of non-coding RNAs, such as vault RNAs (vtRNAs), promoting their processing into regulatory small RNAs. Cytosine C(5)-methylation of vtRNA VTRNA1.1 promotes its processing into small-vault RNA4 (svRNA4) and regulates epidermal differentiation. May act downstream of Myc to regulate epidermal cell growth and proliferation (By similarity). Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity.

PTMs:

Phosphorylated at Ser-139 by AURKB during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1.

細胞の位置付け:

Nucleus>Nucleolus. Cytoplasm. Mitochondrion. Cytoplasm>Cytoskeleton>Spindle. Secreted>Extracellular exosome.
Note: Concentrated in the nucleolus during interphase and translocates to the spindle during mitosis as an RNA-protein complex that includes 18S ribosomal RNA (PubMed:19596847). In testis, localizes to the chromatoid body (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in adult and fetal brain and in lymphoblastoid cells.

サブユニット構造:

Interacts with NPM1 and NCL during interphase; interaction is disrupted following phosphorylation at Ser-139.

タンパク質ファミリー:

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.

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