NOD2 Antibody | Affinity Biosciences

製品:	NOD2 Antibody
カタログ:	DF12125
タンパク質の説明:	Rabbit polyclonal antibody to NOD2
アプリケーション:	WB IHC IF/ICC
反応性:	Human, Mouse, Rat
予測:	Pig, Bovine, Horse, Sheep, Rabbit
分子量:	100-110 kDa; 115kD(Calculated).
ユニプロット:	Q9HC29
RRID:	AB_2844930

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サイズ	価格	在庫状況
100ul	$280	在庫あり
200ul	$350	在庫あり

プロトコル

製品説明

ソース:

Rabbit

アプリケーション:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:

Human,Mouse,Rat

予測:

Pig(92%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%)

クローナリティ:

Polyclonal

特異性:

NOD2 Antibody detects endogenous levels of total NOD2.

RRID:

AB_2844930
引用形式: Affinity Biosciences Cat# DF12125, RRID:AB_2844930.

コンジュゲート:

Unconjugated.

精製:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

別名:

折りたたみ／展開

ACUG; Arthrocutaneouveal granulomatosis; BLAU; CARD15; Caspase recruitment domain family, member 15; Caspase recruitment domain protein 15; Caspase recruitment domain-containing protein 15; CD; CLR16.3; IBD1; Inflammatory bowel disease protein 1; LRR containing protein; NLR family, CARD domain containing 2; NLRC2; NOD like receptor C2; NOD2; NOD2 protein; NOD2_HUMAN; NOD2B; nucleotide binding oligomerization domain 2; Nucleotide binding oligomerization domain containing 2; Nucleotide binding oligomerization domain, leucine rich repeat and CARD domain containing 2; Nucleotide-binding oligomerization domain-containing protein 2; PSORAS1;

免疫原

免疫原:

Uniprot:

Q9HC29(NOD2_HUMAN) >>Visit HPA database.

遺伝子(ID):

NOD2(64127)

発現特異性:

Q9HC29 NOD2_HUMAN:

Expressed in intestinal mucosa, mainly in Paneth cells and, at lower extent, in the glandular epithelium.

タンパク質配列:

Q9HC29 NOD2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGEEGGSASHDEEERASVLLGHSPGCEMCSQEAFQAQRSQLVELLVSGSLEGFESVLDWLLSWEVLSWEDYEGFHLLGQPLSHLARRLLDTVWNKGTWACQKLIAAAQEAQADSQSPKLHGCWDPHSLHPARDLQSHRPAIVRRLHSHVENMLDLAWERGFVSQYECDEIRLPIFTPSQRARRLLDLATVKANGLAAFLLQHVQELPVPLALPLEAATCKKYMAKLRTTVSAQSRFLSTYDGAETLCLEDIYTENVLEVWADVGMAGPPQKSPATLGLEELFSTPGHLNDDADTVLVVGEAGSGKSTLLQRLHLLWAAGQDFQEFLFVFPFSCRQLQCMAKPLSVRTLLFEHCCWPDVGQEDIFQLLLDHPDRVLLTFDGFDEFKFRFTDRERHCSPTDPTSVQTLLFNLLQGNLLKNARKVVTSRPAAVSAFLRKYIRTEFNLKGFSEQGIELYLRKRHHEPGVADRLIRLLQETSALHGLCHLPVFSWMVSKCHQELLLQEGGSPKTTTDMYLLILQHFLLHATPPDSASQGLGPSLLRGRLPTLLHLGRLALWGLGMCCYVFSAQQLQAAQVSPDDISLGFLVRAKGVVPGSTAPLEFLHITFQCFFAAFYLALSADVPPALLRHLFNCGRPGNSPMARLLPTMCIQASEGKDSSVAALLQKAEPHNLQITAAFLAGLLSREHWGLLAECQTSEKALLRRQACARWCLARSLRKHFHSIPPAAPGEAKSVHAMPGFIWLIRSLYEMQEERLARKAARGLNVGHLKLTFCSVGPTECAALAFVLQHLRRPVALQLDYNSVGDIGVEQLLPCLGVCKALYLRDNNISDRGICKLIECALHCEQLQKLALFNNKLTDGCAHSMAKLLACRQNFLALRLGNNYITAAGAQVLAEGLRGNTSLQFLGFWGNRVGDEGAQALAEALGDHQSLRWLSLVGNNIGSVGAQALALMLAKNVMLEELCLEENHLQDEGVCSLAEGLKKNSSLKILKLSNNCITYLGAEALLQALERNDTILEVWLRGNTFSLEEVDKLGCRDTRLLL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Pig

Horse

Rabbit

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HC29 基板として

Q9HC29

Site	PTM Type	Source
T189	Phosphorylation	Uniprot
S238	Phosphorylation	Uniprot
T239	Phosphorylation	Uniprot
S402	Phosphorylation	Uniprot
T424	Phosphorylation	Uniprot
S425	Phosphorylation	Uniprot
S477	Phosphorylation	Uniprot
S493	Phosphorylation	Uniprot
K655	Ubiquitination	Uniprot
K717	Acetylation	Uniprot
K731	Acetylation	Uniprot
S745	Phosphorylation	Uniprot
Y747	Phosphorylation	Uniprot
S983	Phosphorylation	Uniprot
S984	Phosphorylation	Uniprot
T1022	Phosphorylation	Uniprot
S1024	Phosphorylation	Uniprot

研究背景

Q9HC29_NOD2_HUMAN

機能:

Involved in gastrointestinal immunity. Upon stimulation by muramyl dipeptide (MDP), a fragment of bacterial peptidoglycan, binds the proximal adapter receptor-interacting RIPK2, which recruits ubiquitin ligases as XIAP, BIRC2, BIRC3, INAVA and the LUBAC complex, triggering activation of MAP kinases and activation of NF-kappa-B signaling. This in turn leads to the transcriptional activation of hundreds of genes involved in immune response. Required for MDP-induced NLRP1-dependent CASP1 activation and IL1B release in macrophages. Component of an autophagy-mediated antibacterial pathway together with ATG16L1. Plays also a role in sensing single-stranded RNA (ssRNA) from viruses. Interacts with mitochondrial antiviral signaling/MAVS, leading to activation of interferon regulatory factor-3/IRF3 and expression of type I interferon.

PTMs:

Polyubiquitinated following MDP stimulation, leading to proteasome-mediated degradation.

細胞の位置付け:

Cytoplasm. Membrane. Mitochondrion. Basolateral cell membrane.

組織特異性:

Expressed in intestinal mucosa, mainly in Paneth cells and, at lower extent, in the glandular epithelium.

サブユニット構造:

Component of a signaling complex consisting of ARHGEF2, NOD2 and RIPK2. Interacts (via CARD domain) with RIPK2 (via CARD domain). Interacts with ATG16L1. Interacts (via NACHT domain) with CARD9. Interacts with ANKRD17 (via N-terminus). Interacts with HSPA1A; the interaction enhances NOD2 stability. Interacts (via both CARD domains) with HSP90; the interaction enhances NOD2 stability. Interacts (via CARD domain) with SOCS3; the interaction promotes NOD2 degradation. Interacts (via CARD domain) with ERBBI2P; the interaction inhibits activation of NOD2. Interacts (via CARD domain) with CASP1; this interaction leads to IL1B processing. Also interacts with CASP4. Interacts with NLRP1; this interaction is enhanced in the presence of muramyl dipeptide (MDP) and leads to increased IL1B release. Interacts with MAPKBP1; the interaction is enhanced in the presence of muramyl dipeptide (MDP). Interacts with INAVA; the interaction takes place upon PRR stimulation. Interacts with ANKHD1, C10ORF67, CHMP5, DOCK7, ENTR1, KRT15, LDOC1, PPP1R12C, PPP2R3B, TRIM41 and VIM. Interacts with NLRP12; this interaction promotes degradation of NOD2 through the ubiquitin-proteasome pathway.

タンパク質ファミリー:

The ATG16L1-binding motif mediates interaction with ATG16L1.

Intramolecular interactions between the N-terminal moiety and the leucine-rich repeats (LRR) may be important for autoinhibition in the absence of activating signal. In the absence of LRRs, the protein becomes a constitutive activator of CASP1 cleavage and proIL1B processing.

研究領域

· Environmental Information Processing > Signal transduction > TNF signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

参考文献

1). Elevated muramyl dipeptide by sialic acid-facilitated postantibiotic pathobiont expansion contributes to gut dysbiosis-induced mastitis in mice. Journal of Advanced Research, 2024 [IF=11.3]

Application: WB Species: Mouse Sample:

Fig. 5. Enterococcus aggravates gut dysbiosis-induced mastitis through activating NOD2 by MDP production. (A-D) Representative western blot images of NOD2 and NK-κB signaling in the mammary glands from the indicated mice and relative intensity analysis (n = 3). (E and F) Serum MDP concentrations in sialic acid- and E. cecorum-treated mice. (G and H) Mice at E14 were treated with MDP every other day intraperitoneally 10 times until PND14. (G) Representative H&E-stained images of mammary glands and histological analysis. (H) Mammary TNF-α and IL-1β levels and MPO activity were detected in the indicated mice (n = 5). Data are expressed as mean ± SD. *p < 0.05, **p < 0.01 and ***p < 0.001 by one-way ANOVA followed by Tukey’s test (E-G) and two-tailed unpaired Student’s t test (B and D-H). ns, no significance. Scale bars, 50 μm.

2). Cytosolic HMGB1 Mediates LPS-Induced Autophagy in Microglia by Interacting with NOD2 and Suppresses Its Proinflammatory Function. Cells, 2022 (PubMed: 35954253) [IF=6.0]

3). Interferon-γ activated T-cell IRGM–autophagy axis in oral lichen planus. International Immunopharmacology, 2021 (PubMed: 33639564) [IF=5.6]

Application: WB Species: Human Sample: peripheral blood T cells

Fig. 3. Upregulated expression of IRGM and autophagy in peripheral blood T cells of OLP patients. (A-B) Quantitative real-time RT-PCR results showed that IRGM mRNA levels increased in the peripheral blood T cells of OLP (n = 22, P = 0.001), non-erosive OLP (NEOLP, n = 12, P = 0.013) and erosive OLP (EOLP, n = 10, P = 0.033) when compared with that in healthy controls (n = 15), respectively. However, the relative levels of IRGM mRNA showed no significant difference between different clinical types of OLP (P > 0.05). (C-D) Similarly, the relative protein level of IRGM increased in peripheral blood T cells of EOLP (P < 0.001) and NEOLP (P < 0.001) when compared with that in healthy controls. (E-G) Meanwhile, the relative protein level of LC3B upregulated, whereas SQSTM1 and NOD2 protein expression decreased in peripheral blood T cells of all OLP patients (P < 0.05). There were significant differences of expression of LC3B (E, P < 0.001) and SQSTM1 protein (F, P < 0.001) between EOLP and NEOLP groups. The assay was performed in triplicate no less than 3 times. Data were shown as mean ± SEM. * P < 0.05, ** P < 0.01, ***P < 0.001, NS: P > 0.05, nonsignificantly.

4). Novel chimeric TLR2/NOD2 agonist CL429 exhibited significant radioprotective effects in mice. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 33609010) [IF=5.3]

Application: WB Species: mice Sample: intestinal tissues

FIGURE 5 The mechanism for the radioprotective effect of CL429. (A) C57BL/6 mice and TLR2 KO mice were treated with CL429 before TBI, and then, the survival was monitored (WT n = 11, TLR2 KO n = 7). (B) The representative pathological images of haematopoietic system and gastrointestinal tract at 3.5 d after radiation. (C) The protein expression of TLR2 and NOD2 after siRNA knockdown. Cell viability was determined using CCK-8 at 24 h after radiation. *P < .05 vs IR groups. (D) The effects of CL429 on TLR2, NOD2, MyD88 and p-IKK at 0 h, 2 h, 4 h, 8 h, 12 h and 24 h were measured by Western blot assay. (E) IL-6, IL-11, IL-12 and TNF-α were detected by ELISA 24 h after radiation. *P < .05, **P < .01 vs IR + PBS groups

5). An Inflammatory Response-Related Gene Signature Can Impact the Immune Status and Predict the Prognosis of Hepatocellular Carcinoma. Frontiers in Oncology, 2021 (PubMed: 33828988) [IF=4.7]

Application: IHC Species: Human Sample: HCC Tissues and Adjacent Non-Tumorous Tissues

Figure 10 Experiment confirmed the difference of the prognostic gene expression between HCC and adjacent non-tumor tissues. (A) The mRNA expression analysis by qRT-RCR. (B) The protein expression analysis by IHC.

6). Analyses of Transcriptomics upon IL-1β-Stimulated Mouse Chondrocytes and the Protective Effect of Catalpol through the NOD2/NF-κB/MAPK Signaling Pathway. Molecules (Basel, Switzerland), 2023 (PubMed: 36838594) [IF=4.6]

Application: WB Species: Mouse Sample:

Figure 7 The effects of catalpol on the levels of NOD2, IKBα, and P65. (A) NOD2 mRNA level (n = 6); (B) NDD2 protein level; (C) IKBα protein level; (D) P65 protein level (B–D n = 3); above, densitometric analysis; below, (E) representative images of immunoblots. The data are the mean ± S.D. of the mean. * p < 0.05 vs. normal group (N); ** p < 0.01 vs. normal group (N); # p < 0.05 vs. IL-1β group; ## p < 0.01 vs. IL-1β group.

7). Astaxanthin Alleviates Inflammatory Response in Neonatal Necrotizing Enterocolitis Rats by Regulating NOD2/TLR4 Pathway. Gastroenterology research and practice, 2023 (PubMed: 37021078) [IF=2.0]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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NOD2 Antibody - #DF12125

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HPLC Report

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