OGDH Antibody - #DF12127
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12127, RRID:AB_2844932.
折りたたみ／展開
2 oxoglutarate dehydrogenase; 2 oxoglutarate dehydrogenase complex component E1; 2 oxoglutarate dehydrogenase mitochondrial; 2-oxoglutarate dehydrogenase; 2-oxoglutarate dehydrogenase complex component E1; AKGDH; Alpha ketoglutarate dehydrogenase; Alpha-ketoglutarate dehydrogenase; E1k; mitochondrial; ODO1_HUMAN; OGDC; OGDC E1; OGDC-E1; OGDH; Oxoglutarate (alpha ketoglutarate) dehydrogenase (lipoamide); Oxoglutarate decarboxylase; Oxoglutarate dehydrogenase (lipoamide); Oxoglutarate dehydrogenase (succinyl transferring);
免疫原
- Q02218 ODO1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFHLRTCAAKLRPLTASQTVKTFSQNRPAAARTFQQIRCYSAPVAAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLPLSRGSLAAVAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLDESDLDKVFHLPTTTFIGGQESALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDKSSENGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDVKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVMSILLHGDAAFAGQGIVYETFHLSDLPSYTTHGTVHVVVNNQIGFTTDPRMARSSPYPTDVARVVNAPIFHVNSDDPEAVMYVCKVAAEWRSTFHKDVVVDLVCYRRNGHNEMDEPMFTQPLMYKQIRKQKPVLQKYAELLVSQGVVNQPEYEEEISKYDKICEEAFARSKDEKILHIKHWLDSPWPGFFTLDGQPRSMSCPSTGLTEDILTHIGNVASSVPVENFTIHGGLSRILKTRGEMVKNRTVDWALAEYMAFGSLLKEGIHIRLSGQDVERGTFSHRHHVLHDQNVDKRTCIPMNHLWPNQAPYTVCNSSLSEYGVLGFELGFAMASPNALVLWEAQFGDFHNTAQCIIDQFICPGQAKWVRQNGIVLLLPHGMEGMGPEHSSARPERFLQMCNDDPDVLPDLKEANFDINQLYDCNWVVVNCSTPGNFFHVLRRQILLPFRKPLIIFTPKSLLRHPEARSSFDEMLPGTHFQRVIPEDGPAAQNPENVKRLLFCTGKVYYDLTRERKARDMVGQVAITRIEQLSPFPFDLLLKEVQKYPNAELAWCQEEHKNQGYYDYVKPRLRTTISRAKPVWYAGRDPAAAPATGNKKTHLTELQRLLDTAFDLDVFKNFS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q02218 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S24 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| K271 | Acetylation | Uniprot | |
| K276 | Acetylation | Uniprot | |
| Y304 | Phosphorylation | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| T367 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K401 | Acetylation | Uniprot | |
| K402 | Acetylation | Uniprot | |
| T462 | Phosphorylation | Uniprot | |
| K499 | Acetylation | Uniprot | |
| Y527 | Phosphorylation | Uniprot | |
| K532 | Ubiquitination | Uniprot | |
| K534 | Ubiquitination | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| Y555 | Phosphorylation | Uniprot | |
| K561 | Acetylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| K564 | Ubiquitination | Uniprot | |
| K577 | Ubiquitination | Uniprot | |
| K697 | Acetylation | Uniprot | |
| T858 | Phosphorylation | Uniprot | |
| K899 | Acetylation | Uniprot | |
| K899 | Ubiquitination | Uniprot | |
| Y909 | Phosphorylation | Uniprot | |
| T928 | Phosphorylation | Uniprot | |
| S934 | Phosphorylation | Uniprot | |
| K943 | Acetylation | Uniprot | |
| K947 | Ubiquitination | Uniprot | |
| Y966 | Phosphorylation | Uniprot | |
| Y968 | Phosphorylation | Uniprot | |
| K970 | Acetylation | Uniprot | |
| K970 | Ubiquitination | Uniprot | |
| K999 | Acetylation | Uniprot | |
| K999 | Ubiquitination | Uniprot | |
| K1000 | Ubiquitination | Uniprot | |
| T1012 | Phosphorylation | Uniprot |
研究背景
2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex, which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.
Mitochondrion matrix. Nucleus.
Note: Mainly localizes in the mitochondrion. A small fraction localizes to the nucleus, where the 2-oxoglutarate dehydrogenase complex is required for histone succinylation.
The 2-oxoglutarate dehydrogenase complex is composed of OGDH (2-oxoglutarate dehydrogenase; E1), DLST (dihydrolipoamide succinyltransferase; E2) and DLD (dihydrolipoamide dehydrogenase; E3). It contains multiple copies of the three enzymatic components (E1, E2 and E3). In the nucleus, the 2-oxoglutarate dehydrogenase complex associates with KAT2A.
Belongs to the alpha-ketoglutarate dehydrogenase family.
研究領域
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Amino acid metabolism > Lysine degradation.
· Metabolism > Amino acid metabolism > Tryptophan metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.