SEC61G Antibody - #DF12136

(2)   (10)  
製品: SEC61G Antibody
カタログ: DF12136
タンパク質の説明: Rabbit polyclonal antibody to SEC61G
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 7.7 kDa; 8kD(Calculated).
ユニプロット: P60059
RRID: AB_2844941

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関連ダウンロード
MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
SEC61G Antibody detects endogenous levels of total SEC61G.
RRID:
AB_2844941
引用形式: Affinity Biosciences Cat# DF12136, RRID:AB_2844941.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Protein transport protein Sec61 subunit gamma; SC61G_HUMAN; Sec61 gamma subunit; SEC61G; SSS1;

免疫原

免疫原:
Uniprot:

P60059(SC61G_HUMAN) >>Visit HPA database.

遺伝子(ID):
SEC61G(23480)
タンパク質配列:
MDQVMQFVEPSRQFVKDSIRLVKRCTKPDRKEFQKIAMATAIGFAIMGFIGFFVKLIHIPINNIIVGG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P60059 基板として

Site PTM Type Enzyme
M1 Acetylation
K16 Ubiquitination
S18 Phosphorylation

研究背景

機能:

Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across endoplasmic reticulum (ER).

細胞の位置付け:

Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

The ER translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63.

タンパク質ファミリー:

Belongs to the SecE/SEC61-gamma family.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein export.

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

参考文献

1). Identification of dihydrotanshinone I as an ERp57 inhibitor with anti-breast cancer properties via the UPR pathway. BIOCHEMICAL PHARMACOLOGY, 2021 (PubMed: 34062127) [IF=5.8]

Application: WB    Species: Human    Sample: breast cancer cell

Fig. 5. DHT activated ER stress and UPR signaling pathways. MDA-MB-231 cells were incubated with 3 μ M DHT for the indicated time. (A) Western blot analysis was performed with antibodies against Sec61γ, BiP, p-PERK/PERK, p-eIF2 α /eIF2 α , and ATF4. The quantitative densitometric analysis of Sec61γ (B), BiP (C), p-PERK/ PERK (D), p-eIF2 α /eIF2 α (E), and ATF4 (F) was carried out. A representative Western blot is shown (n = 3). *P < 0.05 vs 0 h treatment time point.

Application: WB    Species: human    Sample: MDA-MB-231 cells

Fig. 5.| DHT activated ER stress and UPR signaling pathways. MDA-MB-231 cells were incubated with 3 μM DHT for the indicated time. (A) Western blot analysis was performed with antibodies against Sec61γ, BiP, p-PERK/PERK, p-eIF2α/eIF2α, and ATF4. The quantitative densitometric analysis of Sec61γ (B), BiP (C), p-PERK/PERK (D), p-eIF2α/eIF2α (E), and ATF4 (F) was carried out.
2). SEC61G overexpression and DNA amplification correlates with prognosis and immune cell infiltration in head and neck squamous cell carcinoma. Cancer Medicine, 2021 (PubMed: 34590792) [IF=4.0]

Application: IHC    Species: Human    Sample: oropharyngeal cancer

FIGURE 6 The expression and prognostic values of SEC61G in oropharyngeal cancer: (A, B) representative IHC staining intensities of SEC61G in normal tissues: SEC61G was scored as (A) negative and (B) weak; (C–F) representative IHC staining patterns of SEC61G in OPC tissues: (C) negative, (D) weak, (E) moderate and (F) strong; (G, H) the proportions of SEC61G in normal and tumor tissues; (I) Overall survival rate of SEC61G in oropharyngeal cancer; (J) Progression‐free survival of SEC61G in oropharyngeal cancer

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