TIP47 Antibody - #DF12139

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製品: TIP47 Antibody
カタログ: DF12139
タンパク質の説明: Rabbit polyclonal antibody to TIP47
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 47 kDa; 47kD(Calculated).
ユニプロット: O60664
RRID: AB_2844944

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
TIP47 Antibody detects endogenous levels of total TIP47.
RRID:
AB_2844944
引用形式: Affinity Biosciences Cat# DF12139, RRID:AB_2844944.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

47 kDa mannose 6-phosphate receptor-binding protein; 47 kDa MPR binding protein; 47 kDa MPR-binding protein; Cargo selection protein TIP47; M6PRBP 1; M6PRBP1; Mannose 6 phosphate receptor binding protein 1; Mannose-6-phosphate receptor-binding protein 1; MGC11117; MGC2012; MPR binding protein 47 kDa; Perilipin 3; Perilipin-3; Perilipin3; Placental protein 17; PLIN3; PLIN3_HUMAN; pp17; Tail interacting protein 47 kD; TIP47;

免疫原

免疫原:
Uniprot:

O60664(PLIN3_HUMAN) >>Visit HPA database.

遺伝子(ID):
PLIN3(10226)
タンパク質配列:
MSADGAEADGSTQVTVEEPVQQPSVVDRVASMPLISSTCDMVSAAYASTKESYPHIKTVCDAAEKGVRTLTAAAVSGAQPILSKLEPQIASASEYAHRGLDKLEENLPILQQPTEKVLADTKELVSSKVSGAQEMVSSAKDTVATQLSEAVDATRGAVQSGVDKTKSVVTGGVQSVMGSRLGQMVLSGVDTVLGKSEEWADNHLPLTDAELARIATSLDGFDVASVQQQRQEQSYFVRLGSLSERLRQHAYEHSLGKLRATKQRAQEALLQLSQVLSLMETVKQGVDQKLVEGQEKLHQMWLSWNQKQLQGPEKEPPKPEQVESRALTMFRDIAQQLQATCTSLGSSIQGLPTNVKDQVQQARRQVEDLQATFSSIHSFQDLSSSILAQSRERVASAREALDHMVEYVAQNTPVTWLVGPFAPGITEKAPEEKK

PTMs - O60664 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S31 Phosphorylation
S36 Phosphorylation
Y46 Phosphorylation
K65 Acetylation
K65 Ubiquitination
S83 Phosphorylation
K84 Acetylation
K84 Ubiquitination
S91 Phosphorylation
S93 Phosphorylation
Y95 Phosphorylation
K102 Ubiquitination
K116 Ubiquitination
K122 Acetylation
K122 Sumoylation
K122 Ubiquitination
K128 Ubiquitination
S130 Phosphorylation
K140 Ubiquitination
S148 Phosphorylation
T154 Phosphorylation
S160 Phosphorylation
K164 Acetylation
K164 Ubiquitination
T165 Phosphorylation
S167 Phosphorylation
T170 Phosphorylation
S175 Phosphorylation
S179 Phosphorylation
S187 Phosphorylation
K195 Ubiquitination
S196 Phosphorylation
T207 Phosphorylation
T216 Phosphorylation
S217 Phosphorylation
S241 Phosphorylation
S243 Phosphorylation
R245 Methylation
K283 Ubiquitination
K289 Ubiquitination
S303 Phosphorylation
K307 Ubiquitination
K314 Ubiquitination
T342 Phosphorylation
S347 Phosphorylation
K356 Ubiquitination
T372 Phosphorylation
S374 Phosphorylation
S378 Phosphorylation
S390 Phosphorylation
T412 Phosphorylation
T415 Phosphorylation
K428 Ubiquitination

研究背景

機能:

Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.

細胞の位置付け:

Cytoplasm. Endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lipid droplet.
Note: Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homooligomer. Interacts with M6PR (via the cytoplasmic domain). Interacts with IGF2R (via the cytoplasmic domain). Isoform 2 may exist as a homodimer (known as PP17C).

タンパク質ファミリー:

Belongs to the perilipin family.

参考文献

1). Interface-Targeting Strategy Enables Two-Photon Fluorescent Lipid Droplet Probes for High-Fidelity Imaging of Turbid Tissues and Detecting Fatty Liver. ACS Applied Materials & Interfaces, 2018 (PubMed: 29521495) [IF=9.5]

Application: IF/ICC    Species:    Sample: HeLa cells

Figure S24|Confocal fluorescence images of HeLa cells co@stained with N@Cy and anti@TIP47 antiserum. (1): DIC; (2): N-Cy; (4): Anti@TIP47 antiserum; (4): Merged image of (2) and (3); (5): Merged image of (1) @ (3). (6): The normalized fluorescence profiles of N-Cy and ER red along the white line in (4).N-Cy: 4 UM, 2 min; λex = 488 nm, λem = 510@540 nm. Anti@TIP47 antiserum (fluorescence from Alexa Fluor 594@conjugated goat anti@rabbit IgG): λex = 561 nm; λem = 600@700 nm. Bar = 20 Um.

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