CAPZA1 Antibody - #DF12145
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12145, RRID:AB_2844950.
折りたたみ／展開
Cap Z; Cappa 1; Cappa1; Capping protein (actin filament) muscle Z line alpha 1; Capping protein alpha 1; Capping protein muscle Z line alpha 1; CapZ alpha 1; CapZ alpha-1; CAPZ; CAPZA 1; Capza1; CAZ 1; CAZ1; CAZA1_HUMAN; F actin capping protein alpha 1 subunit; F-actin-capping protein subunit alpha-1;
免疫原
- P52907 CAZA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADFDDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMDQFTPVKIEGYEDQVLITEHGDLGNSRFLDPRNKISFKFDHLRKEASDPQPEEADGGLKSWRESCDSALRAYVKDHYSNGFCTVYAKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFTITPPTAQVVGVLKIQVHYYEDGNVQLVSHKDVQDSLTVSNEAQTAKEFIKIIENAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P52907 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| R7 | Methylation | Uniprot | |
| S9 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| K13 | Ubiquitination | Uniprot | |
| K19 | Acetylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| T63 | Phosphorylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| K97 | Acetylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| K210 | Ubiquitination | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| T224 | Phosphorylation | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| Y239 | Phosphorylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| K256 | Methylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K268 | Sumoylation | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| K273 | Acetylation | Uniprot | |
| K273 | Sumoylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| Y277 | Phosphorylation | Uniprot | |
| K278 | Acetylation | Uniprot | |
| K278 | Sumoylation | Uniprot | |
| K278 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot |
研究背景
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions.
Cytoplasm>Cytoskeleton.
Heterodimer of an alpha and a beta subunit. Interacts with S100A (By similarity). Component of the WASH complex, composed of F-actin-capping protein subunit alpha (CAPZA1, CAPZA2 or CAPZA3), F-actin-capping protein subunit beta (CAPZB), WASH (WASHC1, WASH2P, WASH3P, WASH4P, WASH5P or WASH6P), WASHC2 (WASHC2A or WASHC2C), WASHC3, WASHC4 and WASHC5. Interacts with S100B. Interacts with SH3BP1; recruits CAPZA1 to forming cell junctions. Interacts with CD2AP. Directly interacts with CRACD; this interaction decreases binding to actin.
Belongs to the F-actin-capping protein alpha subunit family.
研究領域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
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