Flotillin 1 Antibody - #DF12155
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12155, RRID:AB_2844960.
折りたたみ／展開
FLOT 1; FLOT1; FLOT1_HUMAN; Flotillin-1; Flotillin1; Integral membrane component of caveolae; Reggie 2;
免疫原
- O75955 FLOT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFFTCGPNEAMVVSGFCRSPPVMVAGGRVFVLPCIQQIQRISLNTLTLNVKSEKVYTRHGVPISVTGIAQVKIQGQNKEMLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O75955 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S19 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y56 | Phosphorylation | P12931 (SRC) | Uniprot |
| K78 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| Y149 | Phosphorylation | P12931 (SRC) | Uniprot |
| K152 | Ubiquitination | Uniprot | |
| Y160 | Phosphorylation | P06241 (FYN) | Uniprot |
| S163 | Phosphorylation | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| Y203 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| K211 | Ubiquitination | Uniprot | |
| Y216 | Phosphorylation | Uniprot | |
| K220 | Methylation | Uniprot | |
| K220 | Ubiquitination | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| Y238 | Phosphorylation | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| Y292 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| Y348 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| T387 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| K422 | Ubiquitination | Uniprot |
研究背景
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
Cell membrane>Peripheral membrane protein. Endosome. Membrane>Caveola>Peripheral membrane protein. Melanosome. Membrane raft.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity).
Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2 (By similarity). Interacts with ECPAS.
Belongs to the band 7/mec-2 family. Flotillin subfamily.
研究領域
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.