MYH4 Antibody - #DF12165
(5)
製品説明
ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(92%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
MYH4 Antibody detects endogenous levels of total MYH4.
RRID:
AB_2844970
引用形式: Affinity Biosciences Cat# DF12165, RRID:AB_2844970.
引用形式: Affinity Biosciences Cat# DF12165, RRID:AB_2844970.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:
折りたたみ／展開
fetal; MYH2B; Myh4; MYH4_HUMAN; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; Myosin heavy chain 4; Myosin heavy chain 4 skeletal muscle; Myosin heavy chain; Myosin heavy chain IIb; Myosin heavy chain skeletal muscle fetal; Myosin-4; skeletal muscle;
免疫原
免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
- Q9Y623 MYH4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSDSEMAIFGEAAPFLRKSEKERIEAQNKPFDAKTSVFVVDPKESYVKAIVQSREGGKVTAKTEAGATVTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEPASGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIEMLLITTNPYDFAFVSQGEITVPSIDDQEELMATDSAVDILGFTADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKSLCYPRVKVGNEFVTKGQTVQQVYNAVGALAKAIYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKPEAHFSLVHYAGTVDYNIAGWLDKNKDPLNETVVGLYQKSAMKTLAFLFSGAQTAEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIEIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAICRGFLMRVEFRKMMERRESIFCIQYNIRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANMKEEFEKTKEELAKTEAKRKELEEKMVTLMQEKNDLQLQVQAEADALADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQMEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLCMDLERAKRKLEGDLKLAQESTMDTENDKQQLNEKLKKKEFEMSNLQGKIEDEQALAIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEESTLQHEATAAALRKKHADSVAELGEQIDSLQRVKQKLEKEKSELKMEINDLASNMETVSKAKANFEKMCRTLEDQLSEIKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEETKAKSTLAHALQSARHDCDLLREQYEEEQEAKAELQRGMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACIALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDHLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKVKKQLDHEKSELQTSLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEELDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKKKMEGDLNEMEIQLNHANRQAAEALRNLRNTQGILKDTQLHLDDAIRGQDDLKEQLAMVERRANLMQAEVEELRASLERTERGRKMAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELESEVESEQKHNVEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKFRKLQHELEEAKERADIAESQVNKLRVKSREVHTKVISEE
種類予測
種類予測:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
92
Rabbit
92
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y623 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T64 | Phosphorylation | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| Y163 | Phosphorylation | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| T198 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| Y269 | Phosphorylation | Uniprot | |
| K273 | Ubiquitination | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| Y362 | Phosphorylation | Uniprot | |
| T381 | Phosphorylation | Uniprot | |
| Y389 | Phosphorylation | Uniprot | |
| Y424 | Phosphorylation | Uniprot | |
| K505 | Acetylation | Uniprot | |
| Y556 | Phosphorylation | Uniprot | |
| T607 | Phosphorylation | Uniprot | |
| Y612 | Phosphorylation | Uniprot | |
| T664 | Phosphorylation | Uniprot | |
| Y719 | Phosphorylation | Uniprot | |
| S732 | Phosphorylation | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| K744 | Acetylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| T790 | Phosphorylation | Uniprot | |
| Y820 | Phosphorylation | Uniprot | |
| K857 | Methylation | Uniprot | |
| K862 | Methylation | Uniprot | |
| K922 | Acetylation | Uniprot | |
| S952 | Phosphorylation | Uniprot | |
| K955 | Acetylation | Uniprot | |
| K956 | Acetylation | Uniprot | |
| T964 | Phosphorylation | Uniprot | |
| T983 | Phosphorylation | Uniprot | |
| T992 | Phosphorylation | Uniprot | |
| T997 | Phosphorylation | Uniprot | |
| K1030 | Methylation | Uniprot | |
| S1041 | Phosphorylation | Uniprot | |
| K1046 | Acetylation | Uniprot | |
| K1087 | Methylation | Uniprot | |
| K1097 | Methylation | Uniprot | |
| K1111 | Acetylation | Uniprot | |
| K1113 | Acetylation | Uniprot | |
| R1130 | Methylation | Uniprot | |
| S1132 | Phosphorylation | Uniprot | |
| S1141 | Phosphorylation | Uniprot | |
| S1144 | Phosphorylation | Uniprot | |
| T1161 | Phosphorylation | Uniprot | |
| S1162 | Phosphorylation | Uniprot | |
| S1185 | Phosphorylation | Uniprot | |
| T1186 | Phosphorylation | Uniprot | |
| T1192 | Phosphorylation | Uniprot | |
| K1223 | Ubiquitination | Uniprot | |
| K1251 | Ubiquitination | Uniprot | |
| S1261 | Phosphorylation | Uniprot | |
| K1264 | Ubiquitination | Uniprot | |
| T1265 | Phosphorylation | Uniprot | |
| S1278 | Phosphorylation | Uniprot | |
| T1286 | Phosphorylation | Uniprot | |
| S1288 | Phosphorylation | Uniprot | |
| S1292 | Phosphorylation | Uniprot | |
| S1303 | Phosphorylation | Uniprot | |
| S1306 | Phosphorylation | Uniprot | |
| T1313 | Phosphorylation | Uniprot | |
| Y1351 | Phosphorylation | Uniprot | |
| S1366 | Phosphorylation | Uniprot | |
| S1370 | Phosphorylation | Uniprot | |
| T1377 | Phosphorylation | Uniprot | |
| Y1379 | Phosphorylation | Uniprot | |
| T1381 | Phosphorylation | Uniprot | |
| T1387 | Phosphorylation | Uniprot | |
| K1394 | Acetylation | Uniprot | |
| K1395 | Acetylation | Uniprot | |
| K1396 | Acetylation | Uniprot | |
| S1413 | Phosphorylation | Uniprot | |
| T1421 | Phosphorylation | Uniprot | |
| Y1464 | Phosphorylation | Uniprot | |
| T1467 | Phosphorylation | Uniprot | |
| S1478 | Phosphorylation | Uniprot | |
| S1480 | Phosphorylation | Uniprot | |
| S1482 | Phosphorylation | Uniprot | |
| T1483 | Phosphorylation | Uniprot | |
| S1514 | Phosphorylation | Uniprot | |
| T1517 | Phosphorylation | Uniprot | |
| S1574 | Phosphorylation | Uniprot | |
| S1600 | Phosphorylation | Uniprot | |
| S1603 | Phosphorylation | Uniprot | |
| T1604 | Phosphorylation | Uniprot | |
| S1714 | Phosphorylation | Uniprot | |
| T1722 | Phosphorylation | Uniprot | |
| T1725 | Phosphorylation | Uniprot | |
| S1726 | Phosphorylation | Uniprot | |
| T1730 | Phosphorylation | Uniprot | |
| K1731 | Acetylation | Uniprot | |
| S1739 | Phosphorylation | Uniprot | |
| T1764 | Phosphorylation | Uniprot | |
| K1774 | Acetylation | Uniprot | |
| K1788 | Acetylation | Uniprot | |
| K1795 | Acetylation | Uniprot | |
| K1813 | Ubiquitination | Uniprot | |
| K1814 | Ubiquitination | Uniprot | |
| T1855 | Phosphorylation | Uniprot | |
| Y1856 | Phosphorylation | Uniprot | |
| K1863 | Acetylation | Uniprot | |
| S1919 | Phosphorylation | Uniprot |
研究背景
機能:
Muscle contraction.
細胞の位置付け:
Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.
サブユニット構造:
Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
タンパク質ファミリー:
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.
研究領域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
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