NUDT21 Antibody - #DF12167
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12167, RRID:AB_2844972.
折りたたみ／展開
CFIM 25; CFIM25; Cleavage and polyadenylation specific factor 5 25 kD subunit; Cleavage and polyadenylation specific factor 5 25 kDa; Cleavage and polyadenylation specific factor 5; Cleavage and polyadenylation specificity factor 25 kDa subunit; Cleavage and polyadenylation specificity factor subunit 5; Cleavage factor Im complex 25 kDa subunit; CPSF 25; CPSF 25 kDa subunit; CPSF 5; CPSF25; CPSF5; CPSF5_HUMAN; DKFZp686H1588; Nucleoside diphosphate linked moiety X motif 21; Nucleoside diphosphate-linked moiety X motif 21; Nudix (nucleoside diphosphate linked moiety X) type motif 21; Nudix hydrolase 21; Nudix motif 21; NUDT 21; Nudt21; Pre mRNA cleavage factor Im (25kD); Pre mRNA cleavage factor Im 25 kDa subunit; Pre mRNA cleavage factor Im 25kD subunit; Pre-mRNA cleavage factor Im 25 kDa subunit;
免疫原
Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
- O43809 CPSF5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVVPPNRSQTGWPRGVTQFGNKYIQQTKPLTLERTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHVLLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGVLQDWVIDDCIGNWWRPNFEPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIISSLPQLLSRFNFIYN
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O43809 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R8 | Methylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| R15 | Methylation | Uniprot | |
| K23 | Acetylation | Uniprot | |
| K23 | Methylation | Uniprot | |
| K23 | Ubiquitination | Uniprot | |
| Y24 | Phosphorylation | Uniprot | |
| K29 | Acetylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| T36 | Phosphorylation | Uniprot | |
| Y40 | Phosphorylation | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K56 | Acetylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K73 | Acetylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| T101 | Phosphorylation | Uniprot | |
| T102 | Phosphorylation | Uniprot | |
| K122 | Acetylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K189 | Acetylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K192 | Ubiquitination | Uniprot |
研究背景
Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA. Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function (By similarity). Binds to chromatin (By similarity). Binds to, but does not hydrolyze mono- and di-adenosine nucleotides.
Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.
Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460). In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles (PubMed:15169763).
Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Homodimer (via N- and C-terminus); binds RNA as homodimer. Component of the cleavage factor Im (CFIm) complex which is a heterotetramer composed of two subunits of NUDT21/CPSF5 and two subunits of CPSF6 or CPSF7 or a heterodimer of CPSF6 and CPSF7. The cleavage factor Im (CFIm) complex associates with the CPSF and CSTF complexes to promote the assembly of the core mRNA 3'-processing machinery. Interacts with CPSF6 (via the RRM domain); this interaction is direct and enhances binding to RNA. Interacts with CPSF7 Ref.30). Interacts with FIP1L1; this interaction occurs in a RNA sequence-specific manner. Interacts with PABPN1. Interacts (via N-terminus) with PAPOLA (via C-terminus); this interaction is direct and diminished by acetylation. Interacts with SNRNP70. Interacts with VIRMA.
Belongs to the Nudix hydrolase family. CPSF5 subfamily.
研究領域
· Genetic Information Processing > Translation > mRNA surveillance pathway.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.