SNX17 Antibody - #DF12172
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12172, RRID:AB_2844977.
折りたたみ／展開
Similar to a putative C elegans gene encoded in cosmid F17H10; Snx17; SNX17_HUMAN; Sorting nexin 17; Sorting nexin-17;
免疫原
- Q15036 SNX17_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MHFSIPETESRSGDSGGSAYVAYNIHVNGVLHCRVRYSQLLGLHEQLRKEYGANVLPAFPPKKLFSLTPAEVEQRREQLEKYMQAVRQDPLLGSSETFNSFLRRAQQETQQVPTEEVSLEVLLSNGQKVLVNVLTSDQTEDVLEAVAAKLDLPDDLIGYFSLFLVREKEDGAFSFVRKLQEFELPYVSVTSLRSQEYKIVLRKSYWDSAYDDDVMENRVGLNLLYAQTVSDIERGWILVTKEQHRQLKSLQEKVSKKEFLRLAQTLRHYGYLRFDACVADFPEKDCPVVVSAGNSELSLQLRLPGQQLREGSFRVTRMRCWRVTSSVPLPSGSTSSPGRGRGEVRLELAFEYLMSKDRLQWVTITSPQAIMMSICLQSMVDELMVKKSGGSIRKMLRRRVGGTLRRSDSQQAVKSPPLLESPDATRESMVKLSSKLSAVSLRGIGSPSTDASASDVHGNFAFEGIGDEDL
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15036 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y37 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| Y51 | Phosphorylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K198 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| T324 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S331 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| T334 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| R339 | Methylation | Uniprot | |
| R341 | Methylation | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| T403 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| S434 | Phosphorylation | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| S437 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S440 | Phosphorylation | Uniprot | |
| T449 | Phosphorylation | Uniprot |
研究背景
Critical regulator of endosomal recycling of numerous surface proteins, including integrins, signaling receptor and channels. Binds to NPxY sequences in the cytoplasmic tails of target cargos. Associates with retriever and CCC complexes to prevent lysosomal degradation and promote cell surface recycling of numerous cargos such as integrins ITGB1, ITGB5 and their associated alpha subunits. Also required for maintenance of normal cell surface levels of APP and LRP1. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)).
Cytoplasm. Early endosome. Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side.
Monomer. Interacts with APP (via cytoplasmic YXNPXY motif) (By similarity). Interacts with KIF1B (By similarity). Interacts with the C-termini of P-selectin, PTC, LDLR, VLDLR, LRP1 and LRP8. Interacts with KRIT1 (via N-terminus). Interacts with HRAS. Interacts with ITGB1 and ITGB5 (via NPxY motif). Interacts with CCDC22, CCDC93, VPS26C and VPS35L; the interaction with VPS26C is direct and associates SNX17 with the retriever and CCC complexes.
The PX domain mediates specific binding to phosphatidylinositol 3-phosphate (PtdIns(P3)). Required for association with endosomes.
The PTB-like F3 module within the FERM-like domain mediates cargo recognition via their NPxY sequences, while the F1 module (Ras-associating) is responsible for interaction with membrane-bound HRAS.
Belongs to the sorting nexin family.
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