VPS33B Antibody - #DF12181
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12181, RRID:AB_2844986.
折りたたみ／展開
FLJ14848; hVPS 33B; hVPS33B; Vacuolar protein sorting 33 homolog B (yeast); Vacuolar protein sorting 33 homolog B; Vacuolar protein sorting 33B (yeast); Vacuolar protein sorting 33B; Vacuolar protein sorting associated protein 33B; VPS 33B;
免疫原
- Q9H267 VP33B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAFPHRPDAPELPDFSMLKRLARDQLIYLLEQLPGKKDLFIEADLMSPLDRIANVSILKQHEVDKLYKVENKPALSSNEQLCFLVRPRIKNMRYIASLVNADKLAGRTRKYKVIFSPQKFYACEMVLEEEGIYGDVSCDEWAFSLLPLDVDLLSMELPEFFRDYFLEGDQRWINTVAQALHLLSTLYGPFPNCYGIGRCAKMAYELWRNLEEEEDGETKGRRPEIGHIFLLDRDVDFVTALCSQVVYEGLVDDTFRIKCGSVDFGPEVTSSDKSLKVLLNAEDKVFNEIRNEHFSNVFGFLSQKARNLQAQYDRRRGMDIKQMKNFVSQELKGLKQEHRLLSLHIGACESIMKKKTKQDFQELIKTEHALLEGFNIRESTSYIEEHIDRQVSPIESLRLMCLLSITENGLIPKDYRSLKTQYLQSYGPEHLLTFSNLRRAGLLTEQAPGDTLTAVESKVSKLVTDKAAGKITDAFSSLAKRSNFRAISKKLNLIPRVDGEYDLKVPRDMAYVFGGAYVPLSCRIIEQVLERRSWQGLDEVVRLLNCSDFAFTDMTKEDKASSESLRLILVVFLGGCTFSEISALRFLGREKGYRFIFLTTAVTNSARLMEAMSEVKA
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9H267 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| K36 | Ubiquitination | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| K321 | Ubiquitination | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| T444 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K470 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| Y501 | Phosphorylation | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| T552 | Phosphorylation | Uniprot | |
| K616 | Ubiquitination | Uniprot |
研究背景
May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating VIPAS39. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical recycling pathway and in the maintenance of the apical-basolateral polarity. Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (By similarity).
Phosphorylated on tyrosine residues. Dephosphorylation by M.tuberculosis PtpA is necessary to induce the reduction of host phagolysosome fusion in M.tuberculosis-infected macrophages.
Late endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lysosome membrane>Peripheral membrane protein>Cytoplasmic side. Early endosome. Cytoplasmic vesicle>Clathrin-coated vesicle. Recycling endosome.
Note: Colocalizes in clusters with VIPAS39 at cytoplasmic organelles (PubMed:19109425). Colocalizes with RAB11A and VIPAS39 on recycling endosomes (PubMed:22753090). Colocalizes with AP-3, clathrin, Rab5 and Rab7b (PubMed:21411634). Colocalizes with M.tuberculosis PtpA in the cytosol of tuberculosis-infected macrophages and associates with phagosomes (PubMed:18474358).
Ubiquitous; highly expressed in testis and low expression in the lung.
Interacts with RAB11A and VIPAS39. Associates with adaptor protein complex 3 (AP-3), clathrin:AP-3 and clathrin:HGS complexes. Interacts with M.tuberculosis PtpA.
Belongs to the STXBP/unc-18/SEC1 family.
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