Collagen Type VI Antibody - #DF12191

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製品: Collagen Type VI Antibody
カタログ: DF12191
タンパク質の説明: Rabbit polyclonal antibody to Collagen Type VI
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Dog, Chicken
分子量: 140 kDa; 109kD(Calculated).
ユニプロット: P12109
RRID: AB_2844996

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 100ul $280 在庫あり
 200ul $350 在庫あり

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Sheep(100%), Dog(100%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
Collagen Type VI Antibody detects endogenous levels of total Collagen Type VI.
RRID:
AB_2844996
引用形式: Affinity Biosciences Cat# DF12191, RRID:AB_2844996.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Alpha 1 (VI) chain (61 AA); CO6A1_HUMAN; COL6A1; Collagen alpha-1(VI) chain; Collagen VI, alpha 1 polypeptide; Collagen, intimal short chain collagen; Collagen, type VI, alpha 1; OPLL; OTTHUMP00000115501;

免疫原

免疫原:

A synthesized peptide derived from human Collagen Type VI, corresponding to a region within the internal amino acids.

Uniprot:

P12109(CO6A1_HUMAN) >>Visit HPA database.

遺伝子(ID):
COL6A1(1291)
タンパク質配列:
MRAARALLPLLLQACWTAAQDEPETPRAVAFQDCPVDLFFVLDTSESVALRLKPYGALVDKVKSFTKRFIDNLRDRYYRCDRNLVWNAGALHYSDEVEIIQGLTRMPGGRDALKSSVDAVKYFGKGTYTDCAIKKGLEQLLVGGSHLKENKYLIVVTDGHPLEGYKEPCGGLEDAVNEAKHLGVKVFSVAITPDHLEPRLSIIATDHTYRRNFTAADWGQSRDAEEAISQTIDTIVDMIKNNVEQVCCSFECQPARGPPGLRGDPGFEGERGKPGLPGEKGEAGDPGRPGDLGPVGYQGMKGEKGSRGEKGSRGPKGYKGEKGKRGIDGVDGVKGEMGYPGLPGCKGSPGFDGIQGPPGPKGDPGAFGLKGEKGEPGADGEAGRPGSSGPSGDEGQPGEPGPPGEKGEAGDEGNPGPDGAPGERGGPGERGPRGTPGTRGPRGDPGEAGPQGDQGREGPVGVPGDPGEAGPIGPKGYRGDEGPPGSEGARGAPGPAGPPGDPGLMGERGEDGPAGNGTEGFPGFPGYPGNRGAPGINGTKGYPGLKGDEGEAGDPGDDNNDIAPRGVKGAKGYRGPEGPQGPPGHQGPPGPDECEILDIIMKMCSCCECKCGPIDLLFVLDSSESIGLQNFEIAKDFVVKVIDRLSRDELVKFEPGQSYAGVVQYSHSQMQEHVSLRSPSIRNVQELKEAIKSLQWMAGGTFTGEALQYTRDQLLPPSPNNRIALVITDGRSDTQRDTTPLNVLCSPGIQVVSVGIKDVFDFIPGSDQLNVISCQGLAPSQGRPGLSLVKENYAELLEDAFLKNVTAQICIDKKCPDYTCPITFSSPADITILLDGSASVGSHNFDTTKRFAKRLAERFLTAGRTDPAHDVRVAVVQYSGTGQQRPERASLQFLQNYTALASAVDAMDFINDATDVNDALGYVTRFYREASSGAAKKRLLLFSDGNSQGATPAAIEKAVQEAQRAGIEIFVVVVGRQVNEPHIRVLVTGKTAEYDVAYGESHLFRVPSYQALLRGVFHQTVSRKVALG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Chicken
83
Horse
73
Xenopus
73
Zebrafish
73
Pig
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Collagen VI acts as a cell-binding protein.

PTMs:

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the type VI collagen family.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Organismal Systems > Digestive system > Protein digestion and absorption.

参考文献

1). circ0066187 promotes pulmonary fibrogenesis through targeting STAT3-mediated metabolism signal pathway. Cellular and molecular life sciences : CMLS, 2025 (PubMed: 39969586) [IF=6.2]

Application: WB    Species: Mouse    Sample:

Fig. 9 Integrative analysis of proteomics and metabolomics. A Venn diagram showed three pathways co-enriched in proteomics and metabolomics, including Protein digestion and absorption, PI3K-Akt signaling pathway, and FoxO signaling pathway. B Interaction network diagram of differential proteins and metabolites among the three pathways. C Western blot verified that DPP4, CCND1, CDK2, and FGF2 were up-regulated and COL6A1 and SOD2 were down-regulated in the TGF-β1-treated MRC-5 cells and BLM-induced mice. D l-Glutamine, l-proline, and AMP increased, and l-arginine, l-phenylalanine, l-lysine, and l-tryptophan decreased in the TGF-β1-treated MRC-5 cells and BLM-induced mice. E Western blot demonstrated that circ0066187 knockdown decreased DPP4, CCND1, CDK2, and FGF2 expression and increased COL6A1 and SOD2 expression compared with those in TGF-β1-treated group. Circ0066187 overexpression enhanced DPP4, CCND1, CDK2, and FGF2 expression, and decreased COL6A1 and SOD2 expression. F miR-29b-2-5p mimic enhanced COL6A1 and SOD2 expression and inhibited DPP4, CCND1, CDK2, and FGF2 expression. miR-29b-2-5p inhibitor enhanced DPP4, CCND1, CDK2, and FGF2 expression and inhibited COL6A1 and SOD2 expression. G Rescue experiment of Western blot demonstrated that the miR-29b-2-5p inhibitor reversed the downward trend of DPP4, CCND1, CDK2, and FGF2 and the upward trend of COL6A1 and SOD2 caused by si-circ0066187. miR-29b-2-5p mimic reversed the upward trend of DPP4, CCND1, CDK2, and FGF2 and the downward trend of COL6A1 and SOD2 caused by circ0066187 overexpression. H Western blot showed that si-STAT3 decreased DPP4, CCND1, CDK2, and FGF2 expression, and increased COL6A1 and SOD2 expression. Overexpressed STAT3 enhanced DPP4, CCND1, CDK2, and FGF2 expression and inhibited COL6A1 and SOD2 expression. I Rescue experiment showed that STAT3 overexpression reversed the downward trend of DPP4, CCND1, CDK2, and FGF2 and the upward trend of COL6A1 and SOD2 caused by si-circ0066187. si-STAT3 reversed the upward trend of DPP4, CCND1, CDK2, and FGF2 and the downward trend of COL6A1 and SOD2 caused by circ0066187 overexpression. NC represents negative control, BP represents blank plasmid, and RP represents the recombinant plasmid of overexpressed circ0066187. OE-STAT3 represents overexpressed STAT3. Each bar represents the mean ± SD; n = 6; *p 
2). FBN1 knockout promotes cervical artery dissection by inducing N-glycosylation alternation of extracellular matrix proteins in rat VSMCs. Cellular signalling, 2023 (PubMed: 37532137) [IF=4.4]

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